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Trans-7,cis-9 CLA is synthesized endogenously by Δ9-desaturase in dairy cowsin dairy cows

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Lipids

Abstract

Cis-9,trans-11 and trans-7,cis-9 CLA are the most prevalent CLA isomers in milkfat. The majority of cis-9,trans-11 CLA is synthesized endogenously by Δ9-desaturase. We tested the hypothesis that trans-7,cis-9 CLA originates from endogenous synthesis by inhibiting Δ9-desaturase with a source of cyclopropene FA (sterculic oil: SO) or with a trans-10,cis-12 CLA supplement. Experiment 1 (four cows; Latin square) involved four treatments: control, SO, partially hydrogenated vegetable oil (PHVO), and PHVO+SO. Milk, plasma, and rumen fluid were collected. Experiment 2 treatments (four cows) were 0 or 14.0 g/d of 10,12 CLA supplement; milk and plasma were collected. Samples were analyzed by GC and Ag+-HPLC to determine FA. In Experiment 1, SO decreased milkfat content of trans-7,cis-9 CLA by 68 to 71% and cis-9,trans-11 CLA by 61 to 65%. In Experiment 2, the 10,12 CLA supplement decreased milkfat content of trans-7,cis-9 CLA and cis-9,trans-11 by 44 and 25%, respectively. Correcting for the extent of treatment-induced inhibition of Δ9-desaturase based on changes in myristic and myristoleic acids, endogenous synthesis of trans-7,cis-9 CLA represented 85 and 102% in Experiments 1 and 2, respectively. Similar corrected values were 77 and 58% for endogenous synthesis of cis-9,trans-11 CLA. Thus, milkfat cis-9,trans-11 CLA was primarily from endogenous synthesis with a minor portion from rumen escape. In contrast, trans-7,cis-9 CLA was not present in rumen fluid in significant amounts. Results indicate this isomer in milkfat is derived almost exclusively from endogenous synthesis via Δ9-desaturase.

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Abbreviations

PHVO:

partially hydrogenated vegetable oil

SO:

sterculic oil

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Authors and Affiliations

  1. Department of Animal Science, University of Helsinki, Helsinki, Finland

    J. Mikko Griinari

  2. Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC

    Pierluigi Delmonte, Kim M. Morehouse & Martin P. Yurawecz

  3. Department of Animal Sciences, University of Arizona, 228 Shantz Bldg., P.O. Box 210038, 85721-0038, Tucson, AZ

    Lance H. Baumgard

  4. Department of Animal Science, Cornell University, 262 Morrison Hall, 4853-4801, Ithaca, NY

    Benjamin A. Corl, Lance H. Baumgard & Dale E. Bauman

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  1. Benjamin A. Corl
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Corl, B.A., Baumgard, L.H., Griinari, J.M. et al. Trans-7,cis-9 CLA is synthesized endogenously by Δ9-desaturase in dairy cowsin dairy cows. Lipids 37, 681–688 (2002). https://doi.org/10.1007/s11745-002-0949-4

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  • DOI: https://doi.org/10.1007/s11745-002-0949-4

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