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Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats

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Lipids

Abstract

The present study compared the effect of dietary conjugated linolenic acid (CLNA) on body fat and serum and liver lipid levels with that of CLA in rats. FFA rich in linoleic acid, α-linolenic acid, CLA, or CLNA were used as experimental fats. Male Sprague-Dawley rats (4 wk old) were fed purified diets containing 1% of one of these experimental fats. After 4 wk of feeding, adipose tissue weights, serum and liver lipid concentrations, serum tumor necrosis factor (TNF)-α and leptin levels, and hepatic β-oxidation activities were measured. Compared with linoleic acid, CLA and, more potently, CLNA were found to reduce perirenal adipose tissue weight. The same trend was observed in the weight of epididymal adipose tissue. CLNA, but not CLA, was found to significantly increase serum and liver IG concentrations. Serum FFA concentration was also increased in the CLNA group more than in the other groups. The activity of β-oxidation in liver mitochondria and peroxisomes was significantly higher in the CLNA group than in the other groups. Thus, the amount of liver TG exceeded the ability of hepatic β-oxidation. Significant positive correlation was found between the adipose tissue weights and serum leptin levels in all animals (vs. perirenal: r=0.557, P<0.001; vs. epididymal: r=0.405, P<0.05). A less significant correlation was found between adipose tissue weights and serum TNF-α level (vs. perirenal: r=0.069, P<0.1; vs. epididymal: r=0.382, P<0.05). Although the mechanism for the specific effect of CLNA is not clear at present, these findings indicate that in rats CLNA modulated the body fat and TG metabolism differently from CLA.

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Abbreviations

CLNA:

conjugated linolenic acid

CPT:

carnitine palmitoyl-transferase

DTNB:

5,5-dithiobis-(2-nitrobenzoic acid)

LA:

linoleic acid

LNA:

α-linolenic acid

PPAR:

peroxisome proliferator-activated receptor

TNF:

tumor necrosis factor

UCP:

uncoupling proteinc

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Authors and Affiliations

  1. Faculty of Enviromental and Symbiotic Sciences, Prefectural University of Kumamoto, 862-8502, Kumamoto, Japan

    Asuka Akahoshi & Michihiro Sugano

  2. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Graduate School Kyushu University, 812-8581, Fukuoka, Japan

    Masao Yamasaki & Koji Yamada

  3. Rinoru Oil Mills Co., Ltd., 103-0027, Tokyo, Japan

    Toshio Iwata, Takeshi Kamegai & Kentaro Tsutsumi

  4. Department of Nutrition, Faculty of Nursing and Nutriton, Siebold University of Nagasaki, 1-1-1 Manabino, 851-2195, Nagayo, Nagasaki, Japan

    Kazunori Koba & Kazunari Tanaka

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  1. Kazunori Koba
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  2. Asuka Akahoshi
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  3. Masao Yamasaki
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Koba, K., Akahoshi, A., Yamasaki, M. et al. Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats. Lipids 37, 343–350 (2002). https://doi.org/10.1007/s11745-002-0901-7

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  • DOI: https://doi.org/10.1007/s11745-002-0901-7

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