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Modulation of arachidonic acid distribution by conjugated linoleic acid isomers and linoleic acid in MCF-7 and SW480 cancer cells

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Lipids

Abstract

The relationship between growth and alterations in arachidonic acid (AA) metabolism in human breast (MCF-7) and colon (SW480) cancer cells was studied. Four different fatty acid preparations were evaluated: a mixture of conjugated linoleic acid (CLA) isomers (c9,t11, t10,c12,c11,t13, and minor amounts of other isomers), the pure c9,t11-CLA isomer, the pure t10,c12-CLA isomer, and linoleic acid (LA) (all at a lipid concentration of 16 μg/mL). 14C-AA uptake into the monoglyceride fraction of MCF-7 cells was significantly increased following 24 h incubation with the CLA mixture (P<0.05) and c9,t11-CLA (P<0.02). In contrast to the MCF-7 cells, 14C-AA uptake into the triglyceride fraction of the SW480 cells was increased while uptake into the phospholipids was reduced following treatment with the CLA mixture (P<0.02) and c9,t11-CLA (P<0.05). Distribution of 14C-AA among phospholipid classes was altered by CLA treatments in both cell lines. The c9,t11-CLA isomer decreased (P<0.05) uptake of 14C-AA into phosphatidylcholine while increasing (P<0.05) uptake into phosphatidylethanolamine in both cell lines. Both the CLA mixture and the t10,c12-CLA isomer increased (P<0.01) uptake of 14C-AA into phosphatidylserine in the SW480 cells but had no effect on this phospholipid in the MCF-7 cells. Release of 14C-AA derivatives was not altered by CLA treatments but was increased (P<0.05) by LA in the SW480 cell line. The CLA mixture of isomers and c9,t11-CLA isomer inhibited 14C-AA conversion to 14C-prostaglandin E2 (PGE2) by 20–30% (P<0.05) while increasing 14C-PGF2α by 17–44% relative to controls in both cell lines. LA significantly (P<0.05) increased 14C-PGD2 by 13–19% in both cell lines and increased 14C-PGE2 by 20% in the SW480 cell line only. LA significantly (P<0.05) increased 5-hydroperoxyeicosatetraenoate by 27% in the MCF-7 cell line. Lipid peroxidation, as determined by increased levels of 8-epi-prostaglandin F2α (8-epi-PGF2α), was observed following treatment with c9,t11-CLA isomer in both cell lines (P<0.02) and with t10,c12-CLA isomer in the MCF-7 cell line only (P<0.05). These data indicate that the growth-promoting effects of LA in the SW480 cell line may be associated with enhanced conversion of AA to PGE2 but that the growth-suppressing effects of CLA isomers in both cell lines may be due to changes in AA distribution among cellular lipids and an altered prostaglandin profile.

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Abbreviations

AA:

arachidonic acid

CLA:

conjugated linoleic acid

5-HPETE:

5-hydroperoxyeicosatetraenoate

IP3 :

inositol triphosphate

LA:

linoleic acid

LTB4 :

leukotriene B4

MG:

monoglyceride

PBS:

phosphate-buffered saline

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

prostaglandin

PGD2 :

prostaglandin D2

PGE2 :

prostaglandin E2

PGF2α :

prostaglandin F2α

PI:

phosphatidylinositol

PKC:

protein kinase C

PL:

phospholipid

PLA2 :

phospholipase A2

PLC:

phospholipase C

PS:

phosphatidylserine

TG:

triglyceride

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Ireland

    Catherine Stanton

  2. School of Biotechnology, Dublin City University, Glasnevin, 9, Dublin, Ireland

    Aine Miller & Rosaleen Devery

Authors
  1. Aine Miller
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  2. Catherine Stanton
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  3. Rosaleen Devery
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Correspondence to Rosaleen Devery.

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Miller, A., Stanton, C. & Devery, R. Modulation of arachidonic acid distribution by conjugated linoleic acid isomers and linoleic acid in MCF-7 and SW480 cancer cells. Lipids 36, 1161–1168 (2001). https://doi.org/10.1007/s11745-001-0827-0

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  • DOI: https://doi.org/10.1007/s11745-001-0827-0

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