Skip to main content

Advertisement

SpringerLink
Log in

Accumulation of metals, antioxidant activity, growth and yield attributes of mustard (Brassica juncea L.) grown on soil amendments with fly ash together with inorganic nitrogen fertilizer

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The study was accompanied to assess the effect of varying levels (0, 20, 40 or 60 t ha−1) of fly ash (FA) and nitrogen (N) (0, 60 or 80 kg ha−1) on Cd, Cr and Pb accumulation, antioxidant activity and yield traits of two mustard cultivars (Brassica juncea cv. ‘Varuna’ and ‘Pusa Bold’). The results showed that antioxidant activity increased concurrently as the FA levels increases. The dose of FA60 accompanied with N60 and N80 lowers enzymatic activity, photosynthetic pigments level, and yield; however, FA at the rate of 40 t ha−1 together with N60 significantly enhanced the crop productivity followed by N80. These results suggest that FA40N60 proved optimal for enhancing crop growth and yield, whereas a high concentration of FA (FA60) caused oxidative stress in both mustard cultivars. Oleic and linoleic acid contents were increased; however, erucic acid content decreased by the application of FA40N60.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. In: Moore PD, Chapman SB (eds) Methods in plant ecology. Blackwell Scientific Publications, Oxford, London, pp 285–344

    Google Scholar 

  • Andresen E, Küpper H (2013) Cadmium toxicity in plants. In: Sigel A, Sigel H, Sigel RKO (eds) Cadmium: from toxicity to essentiality metal ions in life sciences. Springer, New York, pp 395–413

    Chapter  Google Scholar 

  • Antonkiewicz J (2010) Effect of sewage sludge and furnace waste on the content of selected elements in the sward of legume-grass mixture. J Elementol 15:435–443. https://doi.org/10.5601/jelem.2010.15.3.435-443

    Article  Google Scholar 

  • Arivazhagan K, Ravichandran M, Dube SK et al (2011) Effect of coal fly ash on agriculture crops: show case project on use of fly ash in agriculture in and around thermal power station area of national thermal power corporation Ltd., India. World of Coal Ash WOCA, Denver

    Google Scholar 

  • Arnon DI (1949) Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant physiol 24:1–15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Babatunde FE, Yongabi KA (2008) Effect of nitrogen on productivity of Aloe barbadensis L. and its inhibitory effect on Trichophyton rubrum. Adv Hort Sci 22:187–190

    Google Scholar 

  • Bączek-Kwinta R, Bartoszek A, Kusznierewicz B, Antonkiewicz J (2011) Physiological response of plants and cadmium accumulation in heads of two cultivars of white cabbage. J Elementol 16:355–364. https://doi.org/10.5601/jelem.2011.16.3.01

    Article  Google Scholar 

  • Bates LS, Walden RT, Tearse ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteins utilising the principle of protein dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Cakmak I, Horst JH (1991) Effects of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Planta 83:463–468

    Article  CAS  Google Scholar 

  • Chalkoo S, Sahay S, Inam A, Iqbal S (2014) Application of wastewater irrigation on growth and yield of chilli under nitrogen and phosphorus fertilization. J Plant Nutr 37:1139–1147. https://doi.org/10.1080/01904167.2014.881864

    Article  CAS  Google Scholar 

  • Choi IH, Moore P (2008) Effect of various litter amendments on ammonia volatilization and nitrogen content of poultry litter. J Appl Poultry Res 17:454–462

    Article  CAS  Google Scholar 

  • Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T (2007) Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the Cyanobacterium spirulina platensis-S5. Ecotoxicol Environ Saf 66:204

    Article  CAS  PubMed  Google Scholar 

  • Datta JK, Banerjee A, Saha Sikdar M, Gupta S, Mondal NK (2009) Impact of combined exposure of chemical fertilizer, biofertilizer and compost on growth, physiology and productivity of Brassica campestris in old alluvial soil. J Environ Biol 30:797–800

    CAS  PubMed  Google Scholar 

  • Dwivedi RS, Randhawa NS (1974) Evaluation of a rapid test for the hidden hunger of zinc in plants. Plant Soil 40:445–451. https://doi.org/10.1007/BF00011531

    Article  CAS  Google Scholar 

  • Dwivedi S, Tripathi RD, Srivastava S, Mishra S, Shukla MK, Tiwari KK, Singh R, Rai UN (2007) Growth performance and biochemical responses of three rice (Oryza sativa L.) cultivars grown in fly ash amendment soil. Chemosphere 67:140–151

    Article  CAS  PubMed  Google Scholar 

  • Ernst WHO, Verkleij JAC, Schat H (1992) Metal tolerance in plants. Acta Bot Neerl 41:229–248

    Article  CAS  Google Scholar 

  • Fiske CH, Subba Row Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400

    CAS  Google Scholar 

  • Gee GW, Bauder JW (1979) Particle size analysis by hydrometer: a simplified method for routine textural analysis and a sensitivity test of measured parameters. Soil Sci Soc Am J 43:1004–1007

    Article  Google Scholar 

  • Ghosh AB, Bajaj JC, Hasan R, Singh D (1983) Soil and water testing methods: a laboratory manual. Indian Agricultural Research Institute (IARI), New Delhi

    Google Scholar 

  • Gill SS, Khan NA, Tuteja N (2011) Differential cadmium stress tolerance in five Indian mustard (Brassica juncea L.) cultivars. Plant Signal Behav 6:293–300

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gupta DK, Rai UN, Tripathi RD, Inouhe M (2002) Impacts of fly ash on soil and plant responses. J Plant Res 115:401–409

    Article  CAS  PubMed  Google Scholar 

  • Gupta AK, Mishra RK, Sinha S, Lee B (2010) Growth, metal accumulation and yield performance of Brassica campestris L. (cv. Pusa Jaikisan) grown on soil amended with tannery sludge/fly ash mixture. Ecol Eng 36:981–991

    Article  Google Scholar 

  • Hald PM (1946) Notes on determination and distribution of sodium and potassium in cells and serum of normal human blood. J Biol Chem 163:429–434

    CAS  PubMed  Google Scholar 

  • Hazrati S, Sarvestani ZT, Salehi A (2012) The effect of differential nitrogen fertilization on morphological and physiological traits of Aloe vera plants. Int Res J Appl Basic Sci 3:682–687

    Google Scholar 

  • Iqbal S, Tak HI, Inam A, Inam A, Sahay S, Chalkoo S (2015) Comparative effect of wastewater and groundwater irrigation along with nitrogenous fertilizer on growth, photosynthesis and productivity of chilli (Capsicum annuum L.). J Plant Nutr 38:1006–1021. https://doi.org/10.1080/01904167.2014.991032

    Article  CAS  Google Scholar 

  • Jackson ML (1973) Soil chemistry analysis. Prentice Hall, New Delhi

    Google Scholar 

  • Jones GJ, Nichols PD, Johns RB, Smith JD (1987) The effect of mercury and cadmium on the fatty acid and sterol composition of the marine diatoms Asterionella glacialis. Phytochemistry 26:1343–1348

    Article  CAS  Google Scholar 

  • Joshi NL, Mali PC, Saxena A (1998) Effect of nitrogen and sulphur application on yield and fatty acid composition of mustard (Brassica juncea) oil. J Agron Crop Sci 180:59–63

    Article  CAS  Google Scholar 

  • Kaushik N, Agnihotri A (1997) Evaluation of improved method for determination of rapeseed-mustard FAMES by GC. Chromatographia 44:97–99

    Article  CAS  Google Scholar 

  • Keller T, Schwanger H (1977) Air pollution and ascorbic acid. Eur J Pathol 7:338–350

    Article  CAS  Google Scholar 

  • Kuo TM, Somers DA, Kleinhofs A, Warner RL (1982) NADH-nitrate reductase in barley leaves: identification and amino acid composition of subunit protein. Biochim Biophys Acta Protein Str Mol Enzymol 708:75–81

    Article  CAS  Google Scholar 

  • Lindner RC (1944) Rapid analytical method for some of the more common inorganic constituents of plant tissues. Plant Physiol 19:76–89

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu J, Li K, Xu J, Zhang Z, Ma T, Lu X, Yang J, Zhu Q (2004) Lead toxicity, uptake and translocation in different rice cultivars. Plant Sci 165:793–802

    Article  CAS  Google Scholar 

  • Mengel K, Kirkby EA (2001) Principles of plant nutrition, 5th edn. Kluwer Acad. Publishers, Dordrecht, p 849

    Book  Google Scholar 

  • Mishra M, Sahu RK, Padhy RN (2007) Growth, yield, metabolism and elemental status of rice (Oryza sativa L.) Grown in fly ash amended soils. Ecotoxicol 16:271–278

    Article  CAS  Google Scholar 

  • Nakagawa H, Poulle M, Oaks A (1984) Characterization of nitrate reductase from corn leaves (Zea mays cv W64A* W182E): two molecular form of the enzyme. Plant Physiol 75:285–289

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Navari-Izzo F, Quartacci MF (2001) Phytoremediation of metals. Tolerance mechanisms against oxidative stress. Minerva Biotechnol 13:73–83

    Google Scholar 

  • Nazar R, Iqbal N, Syeed S, Khan NA (2011) Salicylic acid alleviates decrease in photosynthesis under salt stress by enhancing nitrogen and sulphur assimilation and antioxidant metabolism differentially in two mungbean cultivars. J Plant Physiol 168:807–815

    Article  CAS  PubMed  Google Scholar 

  • Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH (2012) Glutathione in plants: an integrated overview. Plant Cell Environ 35:454–484

    Article  CAS  PubMed  Google Scholar 

  • Pandey VC, Singh N (2010) Impact of fly ash incorporation in soil systems. Agric Ecosyst Environ 136:16–27

    Article  Google Scholar 

  • Pandey VC, Abhilash PC, Singh N (2009a) The Indian perspective of utilizing fly ash in phytoremediation, phyto-management and biomass production. J Environ Manag 10:2943–2958

    Article  CAS  Google Scholar 

  • Pandey VC, Abhilash PC, Upadhyay RN, Tewari DD (2009b) Application of fly ash on the growth performance, translocation of toxic heavy metals within Cajanus cajan L.: implication for safe utilization of fly ash for agricultural production. J Hazard Mater 166:255–259

    Article  CAS  PubMed  Google Scholar 

  • Pandey VC, Singh JS, Kumar A, Tewari DD (2010) Accumulation of heavy metals by chick pea grown in fly ash treated soil: effects on antioxidants. Clean-Soil Air Water 38:1116–1123

    Article  CAS  Google Scholar 

  • Petruzzelli G, Lubrano L, Cervelli S (1987) Heavy metal uptake by wheat seedlings grown in fly ash amended soils. Water Air Soil Pollut 32:389

    Article  CAS  Google Scholar 

  • Ram LC, Jha SK, Tripathi RC, Masto RE, Selvi VA (2008) Remediation of fly ash landfills through plantation. Remediat J 18:71–90

    Article  Google Scholar 

  • Reynolds M, Foulkes MJ, Slafer GA, Berry P, Parry MAJ, Snape JW, Angus WJ (2009) Raising yield potential in wheat. J Exp Bot 60:1899–1918

    Article  CAS  PubMed  Google Scholar 

  • Romero-Puertas MC, Palma JM, Gómez M, del Río LA, Sandalio LM (2002) Cadmium causes the oxidative modification of proteins in pea plants. Plant Cell Environ 25:677–686

    Article  CAS  Google Scholar 

  • Sahay S, Inam A, Inam S, Iqbal S (2015) Modulation in growth, photosynthesis and yield attributes of black mustard (B. nigra cvIC247) by interactive effect of wastewater and fly ash under different NPK levels. Cogent Food Agric 1:1087632

    Google Scholar 

  • Sakakibara H, Takei K, Hirose N (2006) Interactions between nitrogen and cytokinin in the regulation of metabolism and development. Trends Plant Sci 11:440–448

    Article  CAS  PubMed  Google Scholar 

  • Shanker AK, Carlos C, Loza-Tavera H, Avudainayagam S (2005) Chromium toxicity in plants. Environ Int 31:739–753

    Article  CAS  PubMed  Google Scholar 

  • Singh JS, Pandey VC (2013) Fly ash application in nutrient poor agriculture soils: impact on methanotrophs population dynamics and paddy yields. Ecotoxicol Environ Saf 89:43–51

    Article  CAS  PubMed  Google Scholar 

  • Singh LP, Siddiqui ZA (2003) Effects of fly ash and Helminthosporium oryzae on growth and yield of three cultivars of rice. Bioresour Technol 86:73–78

    Article  CAS  PubMed  Google Scholar 

  • Singh RP, Tripathi RD, Sinha SK, Maheshwari R, Srivastava HS (1997) Response of higher plants to lead contaminated environment. Chemosphere 34:2467–2493

    Article  CAS  PubMed  Google Scholar 

  • Singh PK, Tripathi P, Dwivedi S, Awasthi S, Shri M, Chakrabarty D, Tripathi RD (2016) Fly ash augmented soil enhances heavy metal accumulation and phytotoxicity in rice (Oryza sativa L.); a concern for fly ash amendments in agriculture sector. Plant Growth Regul 78:21–30. https://doi.org/10.1007/s10725-015-0070-x

    Article  CAS  Google Scholar 

  • Sinha SK, Srivastava HS, Tripathi TD (1994) Influence of some growth regulators and divalent cations on the inhibition of nitrate reductase activity by lead in maize leaves. Chemosphere 29:1775–1782

    Article  CAS  Google Scholar 

  • Sinha S, Rai UN, Bhatt K, Pandey K, Gupta AK (2005) Fly ash induced oxidative stress and tolerance in Prosopis juliflora L. grown on different amended substrates. Environ Monit Assess 102:447–457

    Article  CAS  PubMed  Google Scholar 

  • Sinha S, Gupta AK, Bhatt K (2007) Uptake and translocation of metals in fenugreek grown on soil amended with tannery sludge: involvement of antioxidants. Ecotoxicol Environ Saf 672:67–277

    Google Scholar 

  • Tripathi RD, Vajpayee P, Singh N et al (2004) Efficacy of various amendments for amelioration of fly ash toxicity: growth performance and metal composition of Cassia siamea Lamk. Chemosphere 54:1581–1588

    Article  CAS  PubMed  Google Scholar 

  • Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38. https://doi.org/10.1097/00010694-193401000-00003

    Article  CAS  Google Scholar 

  • Xie W, Zhou J, Wang H, Liu Q, Xia J, Lv X (2011) Cu and Pb accumulation in maize (Zea mays L.) and soybean (Glycine max L.) as affected by N, P and K application. Afr J Agric Res 6:1469–1476

    Google Scholar 

  • Yu-Kui R, Fu-Suo Z, Jian-bo S (2009) Effects of nitrogen fertilization on heavy metal content of corn grains. Phyton 78:101–104

    Article  Google Scholar 

Download references

Acknowledgement

Authors are thankful to the University grant Commission, New Delhi for providing financial assistance to carry out this work.

Author information

Authors and Affiliations

  1. Department of Botany, Plant Physiology, Biochemistry and Environmental Botany Section, Aligarh Muslim University, Aligarh, UP, 202002, India

    Farha Ashfaque & Akhtar Inam

Authors
  1. Farha Ashfaque
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akhtar Inam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Farha Ashfaque.

Additional information

Communicated by R. Baczek-Kwinta.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ashfaque, F., Inam, A. Accumulation of metals, antioxidant activity, growth and yield attributes of mustard (Brassica juncea L.) grown on soil amendments with fly ash together with inorganic nitrogen fertilizer. Acta Physiol Plant 42, 150 (2020). https://doi.org/10.1007/s11738-020-03139-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-020-03139-4

Keywords

Search

Navigation