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Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

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Abstract

Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populus trichocarpa (Torr. & Gray) genotype ‘Nisqually-1’. However, ‘Nisqually-1’ has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of ‘Nisqually-1’ was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator-free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0.3% Gelrite® and 5–10 g L−1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

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Abbreviations

AC:

Activated charcoal

BA:

6-Benzyladenine

Chl a :

Chlorophyll a

MS:

Murashige and Skoog

PGR:

Plant growth regulator

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Acknowledgments

This project was supported in part by DOE-Bioenergy Center (BESC) grant, by the US Department of Energy/Oak Ridge National Laboratory (subcontract to Z.-M.C.), and by the Tennessee Agricultural Experiment Station. The BESC is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.

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Authors and Affiliations

  1. Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA

    Byung-guk Kang, Lori Osburn, Dean Kopsell & Zong-Ming Cheng

  2. Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    Gerald A. Tuskan

Authors
  1. Byung-guk Kang
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  2. Lori Osburn
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  3. Dean Kopsell
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  4. Gerald A. Tuskan
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  5. Zong-Ming Cheng
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Correspondence to Zong-Ming Cheng.

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Kang, Bg., Osburn, L., Kopsell, D. et al. Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome. Plant Cell Tiss Organ Cult 99, 251–257 (2009). https://doi.org/10.1007/s11240-009-9596-9

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  • DOI: https://doi.org/10.1007/s11240-009-9596-9

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