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Salt tolerance in two tall wheatgrass species is associated with selective capacity for K+ over Na+

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Abstract

Decaploid Elytrigia elongata (tall wheatgrass) is a halophytic relative of wheat that is used to improve salt tolerance of wheat in China. However, the physiological mechanisms for the salt tolerance of decaploid E. elongata remain elusive. To further gain insights into mechanisms important for salt tolerance, we present here a comparative study of salt tolerance in salt-sensitive tetraploid E. elongata (PI578686) and salt-tolerant decaploid E. elongata (PI276399). Results showed that compared with PI578686, PI276399 exhibited a higher relative growth rate and a stronger selective absorption and -transport capacity for K+ over Na+ under high salt conditions (100–200 mM NaCl). This contributed to maintain lower net Na+ uptake rates and more efficiently control Na+ transport to the shoot in PI276399 than in PI578686. Meanwhile, this also resulted in lower reductions of tissue K+ concentrations as well as of net K+ uptake rates in PI276399 compared to PI578686. Taken together, our findings indicate that PI276399 has the stronger selectivity for K+ over Na+ contributing it to maintain lower Na+ uptake and K+ loss compared with PI578686 in the presence of high salt, and hence endowing the higher salt tolerance of PI276399.

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References

  • Alemán F, Nieves-Cordones M, Martínez V, Rubio F (2009) Potassium/sodium steady-state homeostasis in Thellungiella halophila and Arabidopsis thaliana under long-term salinity conditions. Plant Sci 176:768–774

    Article  Google Scholar 

  • Apse MP, Blumwald E (2007) Na+ transport in plants. FEBS Lett 581:2247–2254

    Article  CAS  PubMed  Google Scholar 

  • Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465:140–151

    Article  CAS  PubMed  Google Scholar 

  • Bose J, Rodrigo-Moreno A, Shabala S (2014) ROS homeostasis in halophytes in the context of salinity stress tolerance. J Exp Bot 65:1241–1257

    Article  CAS  PubMed  Google Scholar 

  • Colmer TD, Epstein E, Dvořák J (1995) Differential solute regulation in leaf blades of various ages in salt-sensitive wheat and a salt-tolerant wheat × Lophopyrum elongatum (Host) A. Love amphiploid. Plant Physiol 108:1715–1724

    CAS  PubMed Central  PubMed  Google Scholar 

  • Colmer TD, Flowers TJ, Munns R (2006) Use of wild relatives to improve salt tolerance in wheat. J Exp Bot 57:1059–1078

    Article  CAS  PubMed  Google Scholar 

  • Deal KR, Goyal S, Dvořák J (1999) Arm location of Lophopyrum elongatum genes affecting K+/Na+ selectivity under salt stress. Euphytica 108:193–198

    Article  CAS  Google Scholar 

  • Demidchik V, Maathuis FJM (2007) Physiological roles of nonselective cation channels in plants: from salt stress to signaling and development. New Phytol 175:387–405

    Article  CAS  PubMed  Google Scholar 

  • Demidchik V, Tester MA (2002) Sodium fluxes through nonselective cation channels in the plant plasma membrane of protoplasts from Arabidopsis roots. Plant Physiol 128:379–387

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Dewey DR (1960) Salt tolerance of 25 strains of Agropyron. Agron J 52:631–635

    Article  Google Scholar 

  • Essah PA, Davenport RJ, Tester M (2003) Sodium influx and accumulation in A. thaliana. Plant Physiol 133:307–318

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945–963

    Article  CAS  PubMed  Google Scholar 

  • Gharsa MA, Parre E, Debez A, Bordenave M, Richard L, Leport L, Bouchereau A, Savouré A, Abdelly C (2008) Comparative salt tolerance analysis between Arabidopsis thaliana and Thellungiella halophila, with special emphasis on K+/Na+ selectivity and proline accumulation. J Plant Physiol 165:588–599

    Article  Google Scholar 

  • Gorham J, Jones RG, Bristol A (1990) Partial characterization of the trait for enhanced K+-Na+ discrimination in the D genome of wheat. Planta 180:590–597

    Article  CAS  PubMed  Google Scholar 

  • Greenway H, Rogers A (1963) Growth and ion uptake of Agropyron elongatum on saline substrates, as compared with a salt-tolerant variety of Hordeum vulgare. Plant Soil 18:21–30

    Article  CAS  Google Scholar 

  • Gulick PJ, Dvořák J (1992) Coordinate gene response to salt stress in Lophopyrum elongatum. Plant Physiol 100:1384–1388

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Guo Q, Wang P, Ma Q, Zhang JL, Bao AK, Wang SM (2012) Selective transport capacity for K+ over Na+ is linked to the expression levels of PtSOS1 in halophyte Puccinellia tenuiflora. Func Plant Biol 39:1047–1057

    Article  CAS  Google Scholar 

  • Guo Q, Meng L, Mao PC, Tian XX (2013a) Role of silicon in alleviating salt-induced toxicity in white clover. Bull Environ Contam Toxicol 91:213–216

    Article  CAS  PubMed  Google Scholar 

  • Guo Q, Meng L, Mao PC, Tian XX (2013b) Role of silicon in alleviating salt-induced toxicity in white clover. Bull Environ Contam Toxicol 91:213–216

    Article  CAS  PubMed  Google Scholar 

  • Hariadi Y, Marandon K, Tian Y, Jacobsen SE, Shabala S (2011) Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) plants grown at various salinity levels. J Exp Bot 62:185–193

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • James RA, Blake C, Byrt CS, Munns R (2011) Major genes for Na+ exclusion Nax1 and Nax2 (wheat HKT1;4 and HKT1;5) decrease Na+ accumulation in bread wheat under saline and waterlogged conditions. J Exp Bot 62:2939–2947

    Article  CAS  PubMed  Google Scholar 

  • Maathuis FJM, Amtmann A (1999) K+ nutrition and Na+ toxicity: the basis of cellular K+/Na+ ratios. Ann Bot 84:123–133

    Article  CAS  Google Scholar 

  • Mao PS, Huang Y, Wang XG, Meng L, Mao PC, Zhang GF (2010) Cytological evaluation and karyotype analysis in plant germplasms of Elytrigia Desv. Agri Sci China 91:1553–1560

    Article  Google Scholar 

  • Martínez JP, Kinet JM, Bajji M, Lutts S (2005) NaCl alleviates polyethylene glycol-induced water stress in the halophyte species Atriplex halimus L. J Exp Bot 419:2421–2431

    Article  Google Scholar 

  • McGuire GE, Dvořák J (1981) High salt tolerance potential in wheat grasses. Crop Sci 21:702–705

    Article  Google Scholar 

  • Meng L, Shang CY, Mao PC, Zhang GF, An SZ (2009) A comprehensive evaluation of salt tolerance for germplasm and materials of Elytrigia at the seeding stage. Acta Pratac Sin 18:67–74 (in Chinese)

    Google Scholar 

  • Mullan DJ, Colmer TD, Francki MG (2007) Arabidopsis–rice–wheat gene orthologues for Na+ transport and transcript analysis in wheat–L. elongatum aneuploids under salt stress. Mol Genet Genomics 277:199–212

    Article  CAS  PubMed  Google Scholar 

  • Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663

    Article  CAS  PubMed  Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of Salinity Tolerance. Annu Rev Plant Biol 59:651–681

    Article  CAS  PubMed  Google Scholar 

  • Peng YH, Zhu YF, Mao YQ, Wang SM, Su WA, Tang ZC (2004) Alkali grass resists salt stress through high [K+] and an endodermis barrier to Na+. J Exp Bot 55:939–949

    Article  CAS  PubMed  Google Scholar 

  • Shabala L, Cuin TA, Newman IA, Shabala S (2005) Salinity-induced ion flux patterns from the excised roots of Arabidopsis sos mutants. Planta 222:1041–1050

    Article  CAS  PubMed  Google Scholar 

  • Shabala S, Demidchik V, Shabala L, Cuin TA, Smith SJ, Miller AJ, Davies JM, Newman IA (2006) Extracellular Ca2+ ameliorates NaCl-induced K+ loss from Arabidopsis root and leaf cells by controlling plasmamembrane K+-permeable channels. Plant Physiol 141:1653–1665

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shan L, LI CL, Chen F, Zhao SY, Xia GM (2008) Bowman-Birk type protease inhibitor is involved in the tolerance to salt stress in wheat. Plant Cell Environ 31:1128–1137

    Article  CAS  PubMed  Google Scholar 

  • Shannon MG (1978) Testing salt tolerance variability among tall wheatgrass lines. Agri J 20:719–722

    Article  Google Scholar 

  • Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–527

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Wang SM, Zhao GQ, Gao YS, Tang ZC, Zhang CL (2005) Puccinellia tenuiflora exhibits stronger selectivity for K+ over Na+ than wheat. J Plant Nutr 27:1841–1857

    Article  Google Scholar 

  • Wang SM, Zhang JL, Flowers TJ (2007) Low affinity Na+ uptake in the halophyte Suaeda maritima. Plant Physiol 145:559–571

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Wang CM, Zhang JL, Liu XS, Li Z, Wu GQ, Cai JY, Flowers TJ, Wang SM (2009) Puccinellia tenuiflora maintains a low Na+ level under salinity by limiting unidirectional Na+ influx resulting in a high selectivity for K+ over Na+. Plant Cell Environ 32:486–496

    Article  CAS  PubMed  Google Scholar 

  • Weimberg R, Shannon MC (1988) Vigor and salt tolerance in 3 lines of tall wheatgrass. Physiol Plant 73:232–237

    Article  CAS  Google Scholar 

  • Wu GQ, Liang N, Feng RJ, Zhang JJ (2013) Evaluation of salinity tolerance in seedlings of sugar beet (Beta vulgaris L.) cultivars using proline, soluble sugars and cation accumulation criteria. Acta Physiol Plant 35:2665–2674

    Article  CAS  Google Scholar 

  • Xia G, Xiang F, Zhou A, Wang H, Chen H (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:305–399

    Article  Google Scholar 

  • Yeo AR, Flowers TJ (1983) Varietal differences in the toxicity of sodium ions in rice leaves. Physiol Plant 59:189–195

    Article  CAS  Google Scholar 

  • Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31272489) and the Scientific Innovation Ability Construction Project of Beijing Academy of Agriculture and Forestry Sciences (BAAFS) (Grant No. KJCX20140103).

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Authors and Affiliations

  1. Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China

    Qiang Guo, Lin Meng, Pei-Chun Mao & Xiao-Xia Tian

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  1. Qiang Guo
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  2. Lin Meng
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  3. Pei-Chun Mao
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  4. Xiao-Xia Tian
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Corresponding authors

Correspondence to Qiang Guo or Lin Meng.

Additional information

Communicated by J. V. Jorrin-Novo.

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Supplementary material 1 (DOCX 90 kb)

Supplementary Fig. 1 Tissue K+/Na+ ratios of salt sensitive tetraploid E. elongata (PI578686) and salt tolerant decaploid E. elongata (PI276399) were exposed to 0, 25, 50, 100, 150 and 200 mM NaCl for 7 d. K+/Na+ ratios in (a) shoot and (b) root of PI578686 and PI276399. Five plants were pooled in each replicate (n = 5). Data are mean ± SE. Different letters indicate significant difference at P < 0.05 (Duncan test).

Supplementary Fig. 2 Root H2O2 contents of salt sensitive tetraploid E. elongata (PI578686) and salt tolerant decaploid E. elongata (PI276399) were exposed to 0, 25, 50, 100, 150 and 200 mM NaCl for 7 d. Five plants were pooled in each replicate (n = 5). Data are mean ± SE. Different letters indicate significant difference at P < 0.05 (Duncan test).

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Guo, Q., Meng, L., Mao, PC. et al. Salt tolerance in two tall wheatgrass species is associated with selective capacity for K+ over Na+ . Acta Physiol Plant 37, 1708 (2015). https://doi.org/10.1007/s11738-014-1708-4

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  • DOI: https://doi.org/10.1007/s11738-014-1708-4

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