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Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors

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Abstract

Linum album has been shown to accumulate some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, we examined the effects of fungal elicitors on the production of lignans in L. album hairy root cultures. The biosynthesis of lignans was differentially affected by fungal elicitors. Fusarium graminearum extract induced the highest increase of PTOX, 190 μg g−1 dry weight (DW), and lariciresinol, 260 μg g−1 DW, which was two-fold and three-fold greater than the untreated control, respectively, while Trichoderma viride extract enhanced the accumulation of MPTOX, instead of PTOX, up to 160 µg g−1 DW, which was 2.4-fold greater than the control. The enhancing effects of fungal elicitors on lignans production was correlated with the increased expression of some key genes involved in the biosynthesis of these compounds, phenylalanine ammonia-lyase, cinnamoyl-CoA reductase, cinnamyl-alcohol dehydrogenase and pinoresinol-lariciresinol reductase.

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Abbreviations

CAD :

Cinnamyl-alcohol dehydrogenase

CCR :

Cinnamoyl-CoA reductase

LC–ESI–MS/MS:

Liquid chromatography–electrospray–mass spectrometry/mass spectrometry

MPTOX:

6-Methoxy podophyllotoxin

PAL :

Phenylalanine ammonia-lyase

PTOX:

Podophyllotoxin

PLR :

Pinoresinol-lariciresinol reductase

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Acknowledgments

We are thankful to Tohru Yamagaki and Ryoichi Araki from Scientific and Technical Services of the Suntory Foundation for Life Sciences, Bioorganic Research Institute, for ESI–MS and qPCR analyses.

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Authors and Affiliations

  1. Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran

    Sedigheh Esmaeilzadeh Bahabadi

  2. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

    Mozafar Sharifi

  3. Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

    Najme Ahmadian Chashmi

  4. Suntory Foundation for Life Sciences, Bioorganic Research Institute, Osaka, Japan

    Jun Murata & Honoo Satake

Authors
  1. Sedigheh Esmaeilzadeh Bahabadi
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  2. Mozafar Sharifi
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  3. Najme Ahmadian Chashmi
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  4. Jun Murata
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  5. Honoo Satake
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Correspondence to Sedigheh Esmaeilzadeh Bahabadi.

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Communicated by E. Kuzniak-Gebarowska.

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Esmaeilzadeh Bahabadi, S., Sharifi, M., Ahmadian Chashmi, N. et al. Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors. Acta Physiol Plant 36, 3325–3331 (2014). https://doi.org/10.1007/s11738-014-1700-z

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  • DOI: https://doi.org/10.1007/s11738-014-1700-z

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