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Growth, morphology and photosynthetic responses of Neochloris oleoabundans during cultivation in a mixotrophic brackish medium and subsequent starvation

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Abstract

The green microalga Neochloris oleoabundans is able to grow in both low and high salinity media and is largely studied for its capability to accumulate lipids under starvation. Moreover, N. oleoabundans is a mixotrophic alga, and then organic carbon addition can promote its growth. This research aims to study the morpho-physiological aspects, with a particular attention on the photosynthetic response, both during mixotrophic growth and starvation in brackish media, more sustainable than freshwater cultivation. In the first step, the alga was cultivated mixotrophically in a brackish medium added with an apple waste product; in the second one, cells were starved also to verify lipid induction. Results indicate that growth is highly promoted during the first week of mixotrophic cultivation, while photosynthetic pigments and lipids are over-produced during the following three weeks of cultivation. In parallel, in mixotrophic cultures the maximum PSII quantum yield was enhanced during the exponential phase of growth. Interesting changes affected the mixotrophic cultures with respect to the partitioning of absorbed light energy. Starvation of both 7-day-grown mixotrophic and autotrophic cultures caused growth inhibition, pigments and photosynthesis downshifting, and concomitantly promoted evident lipid synthesis.

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Abbreviations

A :

Absorbance

AWP:

Apple waste product

Chl:

Chlorophyll

Car:

Carotenoids

F M :

Maximum fluorescence in the dark-adapted state

F M ′ :

Maximum fluorescence in the light-adapted state

F s :

Steady-state fluorescence

F 0 :

Minimum fluorescence

F V :

Variable fluorescence

PAM:

Pulse amplitude modulated fluorimetry

PAR:

Photosynthetically active radiation

PSII:

Photosystem II

TEM:

Transmission electron microscopy

Y(NO):

Combined quantum yield of fluorescence and constitutive thermal dissipation

Y(NPQ):

Quantum yield of regulated energy dissipation

Y(PSII):

Actual PSII quantum yield

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Acknowledgments

This work was financially supported by grants from the Consorzio Universitario Italiano per l’Argentina (CUIA) and from the University of Ferrara.

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Authors and Affiliations

  1. Department of Life Sciences and Biotechnologies, University of Ferrara, Corso Ercole I d’Este 32, 44121, Ferrara, Italy

    Costanza Baldisserotto, Martina Giovanardi, Lorenzo Ferroni & Simonetta Pancaldi

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  1. Costanza Baldisserotto
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  2. Martina Giovanardi
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  3. Lorenzo Ferroni
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  4. Simonetta Pancaldi
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Corresponding author

Correspondence to Simonetta Pancaldi.

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Communicated by B. Zheng.

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Baldisserotto, C., Giovanardi, M., Ferroni, L. et al. Growth, morphology and photosynthetic responses of Neochloris oleoabundans during cultivation in a mixotrophic brackish medium and subsequent starvation. Acta Physiol Plant 36, 461–472 (2014). https://doi.org/10.1007/s11738-013-1426-3

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  • DOI: https://doi.org/10.1007/s11738-013-1426-3

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