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Proteomic analysis of plumules and coleoptiles in maize between hybrids and their corresponding inbred lines

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Abstract

The plumule and coleoptile influence the emergence and vigor of the seedling and demonstrate distinct heterosis during maize (Zea mays L.) development. Comparative two-dimensional analysis was performed on plumules and coleoptiles from 3.5 days-cultivated seedlings of five widely used hybrids in China and their corresponding parental lines to dissect the genetic mechanism of heterosis. Compared to their parental lines, 64 % (2,127/3,310) of the differentially expressed proteins were nonadditively accumulated in the five hybrids. Up-regulated above the high parent, a similar expression pattern in all five tested hybrids, was the major expression pattern accounting for 68 % (1,446/2,127) nonadditive proteins. Forty-two nonadditive protein spots with significant differences between the hybrids and their parental lines were analyzed by mass spectra. Homology searches separated them into six groups with two abundant functional classes of cell detoxification (33 %) and metabolism (26 %), implying their importance for heterotic manifestation of the different hybrids at 3.5 days-cultivated seedling stage. The differential accumulation and expression of herbicide safener protein 1 in cell detoxification implied that exploration of the molecular mechanism of heterosis should be studied in a systematic network. Four potential functional polymorphisms were identified in SBP1 gene providing possible interpretation for the differential expression of SBP1 at transcriptional and translational levels.

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Acknowledgments

The authors are grateful for the grants support from the National Nature Foundation of China (31000715), National Project 973 of China (2012CB723001), the Postdoctoral Foundation of China (20100480852).

Conflict of interest

We (all authors of this paper) declare that there are no financial and commercial conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, College of Agronomy, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China

    Xining Jin, Zhiyuan Fu, Dong Ding, Weihua Li, Zonghua Liu, Yanmin Hu & Jihua Tang

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  1. Xining Jin
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  2. Zhiyuan Fu
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  3. Dong Ding
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  4. Weihua Li
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  6. Yanmin Hu
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  7. Jihua Tang
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Correspondence to Jihua Tang.

Additional information

Communicated by M. Stobiecki.

Xining Jin and Zhiyuan Fu contributed equally to this work.

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Supplementary material 1 (XLS 58 kb)

Table S1. The peptides information of identified proteins. The name, sequence, charge, Xcrr, dCN, Sp of peptides corresponding to each protein were listed.

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Jin, X., Fu, Z., Ding, D. et al. Proteomic analysis of plumules and coleoptiles in maize between hybrids and their corresponding inbred lines. Acta Physiol Plant 36, 355–370 (2014). https://doi.org/10.1007/s11738-013-1417-4

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