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Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds

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Abstract

The effect of hydrogen cyanamide (HC) on dormancy release, antioxidant enzyme’s activity and proline and free polyamine contents were investigated in ‘Superior Seedless’ grapevine buds. HC application caused a sharp decrease of catalase (CAT, EC 1.11.1.6) activity and a transient stimulation during the 5 days following treatment of peroxidase (POD, EC 1.11.1.7) and ascorbate peroxidase (APX, EC 1.11.1.11) activities. This coincided with an accumulation of total free polyamines, especially putrescine (Put). Proline content increased dramatically. There was a strong correlation between APX and POD activities and total free PAs and Put contents implying a possible stimulating effect of the latter compounds on these enzymes. These observations indicate that HC triggers an oxidative stress leading to bud endodormancy release. Afterward, as budbreak started, we observed a rapid proline and Put degradation; this could be responsible for reactivation of growth. Indeed, the decline in Put to (Spd + Spm) ratio, reported here, may be considered as a reliable biochemical marker of bud growth resumption.

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Abbreviations

AcCN:

Acetonitrile

APX:

Ascorbate peroxidase

BSA:

Bovine serum albumin

CAT:

Catalase

DMAB:

3-Dimethylamino benzoic acid

HC:

Hydrogene cyanamide

MBTH:

3-Methyl-2-benzothiazolinone hydrozone hydrochloride monohydrate

PAs:

Polyamines

POD:

Peroxidase

Put:

Putrescine

ROS:

Reactive oxygen species

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

The authors acknowledge the skilful technical assistance of Hilde Verlinden in polyamine analysis.

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Authors and Affiliations

  1. Laboratory of Plant Biotechnology Applied to Crop Improvement, Department of Life Sciences, Faculty of Sciences of Gabès, University of Gabès, Cité Erriadh, Zrig, 6072, Gabès, Tunisia

    Hatem Ben Mohamed, Ahmedou M. Vadel & Habib Khemira

  2. Laboratory of Functional Biology, KU leuven, Kasteelpark Arenberg 31, 3001, Heverlee, Belgium

    Jan M. C. Geuns

Authors
  1. Hatem Ben Mohamed
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  2. Ahmedou M. Vadel
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  3. Jan M. C. Geuns
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  4. Habib Khemira
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Correspondence to Habib Khemira.

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Communicated by M. Horbowicz.

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Ben Mohamed, H., Vadel, A.M., Geuns, J.M.C. et al. Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds. Acta Physiol Plant 34, 429–437 (2012). https://doi.org/10.1007/s11738-011-0839-0

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  • DOI: https://doi.org/10.1007/s11738-011-0839-0

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