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Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI

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Abstract

Endogenous indoleamine profiles in various ex vitro and in vitro tissues of commercially important Coffea canephora were analyzed by using a high performance liquid chromatography and further confirmed with electrospray ionization mass spectrometry. High content of serotonin (SER) (98.54 ± 5 μg/g) and melatonin (MEL) (115.25 ± 6 μg/g) were found in freshly harvested seeds of C. canephora followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 μg/g fresh weight) and endosperm (34.08 ± 2 and 51.08 ± 4 μg/g fresh weight) of ripened fruits. Similarly endogenous pools of SER and MEL were moderate in in vitro tissues of C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic embryos (31.88 ± 2 and 19.30 ± 2 μg/g fresh weight) and in vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 μg/g fresh weight), respectively. In view of significant levels of both SER and MEL in various tissues and beans of Coffea, further investigations on their physiological role needs to be investigated.

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Abbreviations

LC-MS-ESI:

Liquid chromatography mass spectrometry electrospray ionization

MS:

Murashige and Skoog

MEL:

Melatonin

SER:

Serotonin

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Acknowledgments

The authors are thankful to Department of Science and Technology, Government of India, New Delhi for financial assistance. RA acknowledges CSIR, New Delhi for awarding Senior Research Fellowship.

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Authors and Affiliations

  1. Plant Cell Biotechnology Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research), Mysore, 570 020, India

    Akula Ramakrishna, Parvatam Giridhar, Kadimi Udaya Sankar & Gokare Aswathanarayana Ravishankar

  2. Food Engineering Department, Central Food Technological Research Institute (Constituent Laboratory of Council of Scientific and Industrial Research), Mysore, 570 020, India

    Kadimi Udaya Sankar

Authors
  1. Akula Ramakrishna
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  2. Parvatam Giridhar
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  3. Kadimi Udaya Sankar
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  4. Gokare Aswathanarayana Ravishankar
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Corresponding author

Correspondence to Gokare Aswathanarayana Ravishankar.

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Communicated by J. Van Huylenbroeck.

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Ramakrishna, A., Giridhar, P., Sankar, K.U. et al. Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. Acta Physiol Plant 34, 393–396 (2012). https://doi.org/10.1007/s11738-011-0829-2

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