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Identification of sequences expressed during compatible black pepper—Fusarium solani f. sp. piperis interaction

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Abstract

Black pepper (Piper nigrum L.) is one of the most widely used spices in the world. Root rot disease is induced by Fusarium solani f. sp. piperis and causes severe yield losses of this crop in the Amazon region. In this work we used the suppression subtractive hybridization to identify differentially expressed sequences in roots of black pepper infected by F. solani f. sp. piperis. Sequences coding for putative proteins related to oxidative burst and defense response, such as superoxide dismutase, cytochrome p450, and alpha-amylase inhibitors/lipid transfer protein, comprised 28.4% of SSH clones according to computational analyses. Furthermore, semi-quantitative RT-PCR assays showed accumulation of putative cysteine proteinase inhibitor and pathogenesis-related protein 4 transcripts at late stage of infection that can help to explain the success of this pathogen in causing root rot disease in black pepper. The results obtained here contribute to improve our understanding about this plant–pathogen interaction.

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Acknowledgments

Research supported by: the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), the Fundação de Amparo à Pesquisa do Estado do Pará (FAPESPA), and the Universidade Federal do Pará (UFPA), Brazil. The authors thank Maria de Lourdes Reis Duarte for providing the fungus strain and Soelange Bezerra do Nascimento for help in the plant material preparation.

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Authors and Affiliations

  1. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, Belém, PA, 66075-110, Brazil

    Cláudia Regina Batista de Souza, Ailton Borges Santa Brígida, Rafaela Cabral dos Santos, Carinne de Nazaré Monteiro Costa, Sylvain Henri Darnet & Maria Lúcia Harada

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  1. Cláudia Regina Batista de Souza
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  2. Ailton Borges Santa Brígida
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  3. Rafaela Cabral dos Santos
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  4. Carinne de Nazaré Monteiro Costa
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  5. Sylvain Henri Darnet
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  6. Maria Lúcia Harada
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Correspondence to Cláudia Regina Batista de Souza.

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Communicated by T. Moriguchi.

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de Souza, C.R.B., Brígida, A.B.S., Santos, R.C.d. et al. Identification of sequences expressed during compatible black pepper—Fusarium solani f. sp. piperis interaction. Acta Physiol Plant 33, 2553–2560 (2011). https://doi.org/10.1007/s11738-011-0788-7

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  • DOI: https://doi.org/10.1007/s11738-011-0788-7

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