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A new pathogenesis-related protein, LrPR4, from Lycoris radiata, and its antifungal activity against Magnaporthe grisea

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Abstract

A new Lycoris radiata pathogenesis-related (PR)-4 gene, LrPR4 was isolated. LrPR4 encodes a 142 amino acid protein with a predicted molecular mass of 15.43 kDa and pI of 7.56. The putative LrPR4 shows high similarity to PR4 type proteins from various plant species and belongs to the Barwin family. Like other PR4s from monocot plants, LrPR4 protein contains a conserved Barwin domain and has a signal peptide at its N-terminus. The recombinant LrPR4 protein expressed in Escherichia coli showed activity towards hydrolysing RNA from L. radiata bulbs and antifungal activity. The results of this study suggest that LrPR4 may play a role in the disease resistance responses of plant against pathogen attacks though its antifungal activity.

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References

  1. Fritig B, Kauffmann S, Dumas B, Geoffrey P, Kopp M, Legrand M (1987) Mechanism of the hypersensitive reaction of plants. In: Evered D, Harnett S (eds) Plant resistance to viruses, CIBA Foundation Symposia, vol 133. Wiley, Chichester, pp 92–108

    Chapter  Google Scholar 

  2. Neuhans JM (1999) Plant chitinases. In: Datta SK, Muthukrishnan S (eds) Pathogenesis-related proteins in plants. CRC Press, Boca Baton

    Google Scholar 

  3. Van Loon LC, Van Strien EA (1999) The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol Mol Plant Pathol 55:85–97

    Article  Google Scholar 

  4. Christensen AB, Cho BH, Naesby M, Gregersen PL, Brandt J, Madriz-Ordenana K, Collinge DB, Thordal-Christensen H (2002) The molecular characterization of two barley proteins establishes the novel PR-17 family of pathogenesis related proteins. Mol Plant Pathol 3:135–144

    Article  CAS  Google Scholar 

  5. Liu JJ, Ekramoddollah AKM (2006) The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stress. Physiol Mol Plant Pathol 68:3–13

    Article  CAS  Google Scholar 

  6. Van Loon LC, Rep M, Pieterse CMJ (2006) Significance of inducible defense-related proteins in infected plants. Ann Rev Phytopathol 44:135–162

    Article  Google Scholar 

  7. Agrawal GK, Jwa NS, Han KS, Agrawal VP, Rakwal R (2003) Isolation of a novel rice PR4 type gene whose mRNA expression is modulated by blast pathogen attack and signaling components. Plant Physiol Biochem 41:81–90

    Article  CAS  Google Scholar 

  8. Caruso C, Nobile M, Leonardi L, Bertini L, Buonocore V, Caporale C (2001) Isolation and amino acid sequence of two new PR-4 proteins from wheat. J Protein Chem 20:327–335

    Article  CAS  PubMed  Google Scholar 

  9. Zhu T, Song F, Zheng Z (2006) Molecular characterization of the rice pathogenesis-related protein, OsPR-4b, and its antifungal activity against Rhizoctonia solani. J Phytopathol 154:378–384

    Article  CAS  Google Scholar 

  10. Friedrich L, Moyer M, Ward E, Ryals J (1991) Pathogenesis-related protein 4 is structurally homologous to the carboxy-terminal domains of hevein, Win-1 and Win-2. Mol Gen Genet 230:113–119

    Article  CAS  PubMed  Google Scholar 

  11. Hejgaard J, Jacobsen S, Bjorn SE, Kragh KM (1992) Antifungal activity of chitin-binding PR-4 type proteins from barley grain and stressed leaf. FEBS Lett 307:389–392

    Article  CAS  PubMed  Google Scholar 

  12. Ponstein AS, Bres-Vloemans SA, Sela-Buurlage MB, van den Elzen PJ, Melchers LS, Cornelissen BJ (1994) A novel pathogen- and wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiol 104:109–118

    Article  CAS  PubMed  Google Scholar 

  13. Van Damme EJ, Charels D, Roy S, Tierens K, Barre A, Martins JC, Rouge P, Van Leuven F, Does M, Peumans WJ (1999) A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes. Plant Physiol 119:1547–1556

    Article  PubMed  Google Scholar 

  14. Svensson B, Svendsen I, Hojrup P, Roepstorff P, Ludvigsen S, Poulsen FM (1992) Primary structure of Barwin: a barley seed protein closely related to the C-terminal domain of proteins encoded by wound-induced plant genes. Biochem 31:8767–8770

    Article  CAS  Google Scholar 

  15. Caruso C, Caporale C, Chilosi G, Vacca F, Berini L, Magro P, Poerio E, Buonocore V (1996) Structural and antifungal properties of a pathogenesis-related protein from wheat kernel. J Prot Chem 15:35–44

    Article  CAS  Google Scholar 

  16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680

    Article  CAS  PubMed  Google Scholar 

  17. Bantignies B, Seguin J, Muzac I, Dedaldechamp F, Gulick P, Ibrahim R (2000) Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots. Plant Mol Biol 42:871–881

    Article  CAS  PubMed  Google Scholar 

  18. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 23:3389–3402

    Article  Google Scholar 

  19. Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  Google Scholar 

  20. Guex N, Peitsch MC (1997) Swiss-model and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18:2714–2723

    Article  CAS  PubMed  Google Scholar 

  21. Rost B, Sander C (1994) Conservation and prediction of solvent accessibility in protein families. Proteins 20:216–226

    Article  CAS  PubMed  Google Scholar 

  22. Neuhaus JM, Sticher L, Meins F Jr, Boller T (1991) A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc Natl Acad Sci 88:10362–10366

    Article  CAS  PubMed  Google Scholar 

  23. Van Parijs J, Broekaert WF, Goldstein IJ, Peumans WJ (1991) Hevein: an antifungal protein from rubber-tree (Hevea brasiliensis) latex. Planta 183:258–264

    Article  Google Scholar 

  24. Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K (1993) Plant chitinases. Plant J 3:31–40

    Article  CAS  PubMed  Google Scholar 

  25. Coupe SA, Taylor JE, Roberts JA (1997) Temporal and spatial expression of mRNAs encoding pathogenesis-related proteins during ethylene-promoted leaflet abscission in Sambucus nigra. Plant Cell Environ 20:1517–1524

    Article  CAS  Google Scholar 

  26. Ou SH (1985) Rice disease. Common wealth Mycological Institute, Surrey, pp 8–24

    Google Scholar 

  27. De Warrd MA, Georgopoulas SG, Hollomon DW, Ishii H, Leroux P, Ragsdale NM, Schwinn FJ (1993) Chemical control of plant diseases: problems and prospects. Annu Rev Phytopathol 31:403–421

    Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant no. 30700057), the opening fund of Jiangsu province key laboratory for plant Ex-situ conservation (KF07001). We wish to thank Dr. Evan Evans from Tasmanian Institute of Agricultural Research at University of Tasmania and Prof. Wenbiao Shen from the College Life Sciences at Nanjing Agricultural University for critical reading and linguistic help in the preparation of this manuscript.

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Authors and Affiliations

  1. Qian Hu Hou Cun 1#, Jiangsu Province Key Laboratory for Plant Ex-Situ Conservation, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, 210014, Nanjing, People’s Republic of China

    Xiaodan Li, Bing Xia, Yumei Jiang, Chunyan Wang, Lisi He, Feng Peng & Ren Wang

  2. South Subtropical Crops Research Institute (SSCRI), Chinese Academy of Tropical Agricultural Sciences (CATAS), 524091, Zhanjiang, China

    Qingsong Wu

  3. College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Lisi He

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  1. Xiaodan Li
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  8. Ren Wang
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Correspondence to Ren Wang.

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Li, X., Xia, B., Jiang, Y. et al. A new pathogenesis-related protein, LrPR4, from Lycoris radiata, and its antifungal activity against Magnaporthe grisea . Mol Biol Rep 37, 995–1001 (2010). https://doi.org/10.1007/s11033-009-9783-0

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