Skip to main content
SpringerLink
Log in

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Soluble acid invertase (SAI, EC 3.2.1.26), catalyzes the hydrolysis of sucrose into hexose sugars, and it has been considered a key enzyme for carbohydrate metabolism. In the present study, the activity of SAI enzyme was determined to establish a correlation between the change in transcript levels and enzyme activity in high and low sugar accumulating sugarcane cultivars, in various internodal tissues at different developmental stages. A decrease in SAI activity and transcript levels was observed with age, during all the developmental stages in both the cultivars. A negative correlation between SAI activity and sucrose content was observed in mature and immature internodes; however, there was a positive correlation between SAI activity and content of hexose sugars. These results imply that SAI plays a crucial role in sucrose partitioning in various intermodal tissues in high and low sugar cultivars. In addition to this, the changes in enzyme activity also resulted in changes in transcript level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

SAI:

Soluble acid invertase

DAP:

Days after planting

FW:

Fresh weight

References

  • Batta SK, Singh R (1986) Sucrose metabolism in sugarcane grown under varying climatic conditions: synthesis and storage of sucrose in relation to the activities of sucrose synthase, sucrose phosphate synthase and invertase. Phytochemistry 25:2431–2437

    Article  CAS  Google Scholar 

  • Botha FC, Black KG (2000) Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Aust J Plant Physiol 27:81–85

    CAS  Google Scholar 

  • Burg SP, Bieleski RL (1962) The physiology of sugarcane. V. Kinetics of sugar accumulation. Aust J Biol Sci 15:429–444

    Google Scholar 

  • Carson DL, Botha FC (2002) Genes expressed in sugarcane maturing internodal tissue. Plant Cell Rep 20:1075–1081

    Article  CAS  Google Scholar 

  • Carson DL, Huckett BI, Botha FC (2002) Sugarcane ESTs differentially expressed in immature and maturing internodal tissue. Plant Sci 162:289–300

    Article  CAS  Google Scholar 

  • Casu RE, Jarmey JM, Bonnett GD, Manners JM (2007) Identification of transcripts associated with cell wall metabolism and development in the stem of sugarcane by Affymetrix GeneChip Sugarcane Genome Array expression profiling. Funct Integr Genomics 7:153–167

    Article  PubMed  CAS  Google Scholar 

  • Echeverria E, Gonzalez PC, Brune A (1997) Characteristics of proton and sugar transport at the tonoplast of sweet lime (Citrus limettioides) juice cells. Physiol Plant 101:291–300

    Article  CAS  Google Scholar 

  • Glasziou KT, Gayler KR (1972) Storage of sugars in stalks of sugarcane. Bot Rev 38:471–490

    Article  Google Scholar 

  • Grof CPL, Campbell JA (2001) Sugarcane sucrose metabolism: scope for molecular manipulation. Aust J Plant Physiol 28:1–12

    CAS  Google Scholar 

  • Hawker JS, Hatch MD (1965) Mechanism of sugar storage by mature stem tissue of sugarcane. Plant Physiol 18:444–453

    Article  Google Scholar 

  • Klann EM, Hall B, Bennett AB (1996) Antisense acid invertase (T1V1) gene alters soluble sugar composition and size in transgenic tomato fruit. Plant Physiol 112:1321–1330

    Article  PubMed  CAS  Google Scholar 

  • Kubo T, Hohjo I, Hiratsuka S (2001) Sucrose accumulation and its related enzyme activities in the juice sacs of Satsuma mandarin fruit from trees of different crop loads. Sci Hortic 91:215–225

    Article  CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • Ma H, Albert HH, Paull R, Moore PH (2000) Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells. Aust J Plant Physiol 27:1021–1030

    CAS  Google Scholar 

  • Martinoia E (1992) Transport process in vacuoles of higher plants. Bot Acta 105:232–245

    CAS  Google Scholar 

  • McCormick AJ, Cramer MD, Watt DA (2006) Sink strength regulates photosynthesis in sugarcane. New Phytol 171:759–770

    Article  PubMed  CAS  Google Scholar 

  • Menossi M, Silva-Filho MC, Vincentz M, Van-Sluys MA, Souza GM (2008) Sugarcane functional genomics: gene discovery for agronomic trait development. Int J Plant Genomics 1:11

    Google Scholar 

  • Moore PH (1995) Temporal and spatial regulation of sucrose accumulation in the sugarcane stem. Aust J Plant Physiol 22:661–679

    Article  CAS  Google Scholar 

  • Moore PH (2005) Integration of sucrose accumulation processes across hierarchical scales: towards developing an understanding of the gene to crop continuum. Field Crop Res 92:119–135

    Article  Google Scholar 

  • Nelson N (1944) A photometric adaptation of Somogyi method for the determination of glucose. J Biol Chem 153:375–380

    CAS  Google Scholar 

  • Quick WP, Schaffer AA (1996) Sucrose metabolism in sources and sinks. In: Zamski E, Schaffer AA (eds) Photoassimilate distribution in plants and crops. Marcel Dekker, NY, pp 115–156

    Google Scholar 

  • Rausch T, Greiner S (2004) Plant protein inhibitors of invertases. Biochim Biophys Acta 1696:253–261

    PubMed  CAS  Google Scholar 

  • Roe JH (1934) A colorimetric method for the determination of fructose in blood and urine. J Biol Chem 107:15–22

    CAS  Google Scholar 

  • Sacher JA, Hatch MD, Glasziou KT (1963) Sugar accumulation cycle in sugarcane. III. Physical and metabolic aspect of cycle in immature storage tissues. Plant Physiol 39:348–354

    Article  Google Scholar 

  • Scholes J, Bundock N, Wilde R, Rolfe S (1996) The impact of reduced vacuolar invertase on the photosynthetic and carbohydrate metabolism of tomato. Planta 200:265–272

    Article  CAS  Google Scholar 

  • Singh RK, Singh P, Singh SP, Mohapatara T, Singh SB (2006) Mapping QTLs for sugar content and segregation analysis in sugarcane. Sugar Cane Int 24:7–13

    CAS  Google Scholar 

  • Tang GQ, Luscher M, Sturm A (1999) Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose portioning. Plant Cell 11:177–190

    Article  PubMed  CAS  Google Scholar 

  • Vattuone MA, Fleischmacher OL, Prado FE, Vinals AL, Sampietro AR (1983) Localisation of invertase activities in Ricinus communis leaves. Phytochemistry 22:1361–1365

    Article  CAS  Google Scholar 

  • Venkataramana S, Naidu MK, Singh S (1991) Invertase and growth factors dependent sucrose accumulation in sugarcane. Plant Sci 74:65–72

    Article  CAS  Google Scholar 

  • Verma AK, Upadhyay SK, Verma PC, Solomon S, Singh SB (2010) Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biol. doi:10.1111/j.1438-8677.2010.00379.x

  • Walker RP, Pollock CJ (1993) The purification and characterisation of soluble acid invertase from coleoptiles of wheat (Triticum aestivum L. cv. Avalon). J Exp Bot 44:1029–1037

    Article  CAS  Google Scholar 

  • Welbaum GE, Meinzer FC (1990) Compartmentation of solutes and water in developing sugarcane stalk tissue. Plant Physiol 93:1147–1153

    Article  PubMed  CAS  Google Scholar 

  • Whittaker A, Botha FC (1997) Carbon partitioning during sucrose accumulation in sugarcane internodal tissues. Plant Physiol 115:1651–1659

    PubMed  CAS  Google Scholar 

  • Winter H, Robinson DG, Heldt HW (1994) Subcellular volumes and metabolites in spinach leaves. Planta 193:530–535

    Article  CAS  Google Scholar 

  • Yamada K, Kojima T, Bantog N, Shimoda T, Mori H, Shiratake K, Yamaki S (2007) Cloning of two isoforms of soluble acid invertase of Japanese pear and their expression during fruit development. J Plant Physiol 164:746–755

    Article  PubMed  CAS  Google Scholar 

  • Zhu YJ, Komor E, Moore PH (1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol 115:609–616

    Article  PubMed  CAS  Google Scholar 

  • Zhu YJ, Albert HH, Moore PH (2000) Differential expression of soluble acid invertase (SAI) genes correlates to differences in sucrose accumulation in sugarcane. Aust J Plant Physiol 27:193–199

    CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Sugar Development Fund, Ministry of Food and Consumer Affairs under the Government of India for the research grant provided for the research project.

Author information

Authors and Affiliations

  1. U.P. Council of Sugarcane Research, Shahjahanpur, 242001, India

    Ashok K. Verma & S. B. Singh

  2. Plant Molecular Biology Laboratory, National Botanical Research Institute, Lucknow, 226001, India

    Ashok K. Verma, Santosh K. Upadhyay & Praveen C. Verma

  3. Indian Institute of Sugarcane Research, Lucknow, India

    S. Solomon

  4. Key Laboratory of Genetic Crop Improvement and Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, People’s Republic of China

    Manoj K. Srivastava

Authors
  1. Ashok K. Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Santosh K. Upadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Manoj K. Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Praveen C. Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Solomon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. B. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ashok K. Verma.

Additional information

Communicated by P. Sowinski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verma, A.K., Upadhyay, S.K., Srivastava, M.K. et al. Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane. Acta Physiol Plant 33, 1749–1757 (2011). https://doi.org/10.1007/s11738-011-0711-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-011-0711-2

Keywords

Search

Navigation