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The contribution of sucrose metabolism enzymes to sucrose accumulation in sugarcane (Saccharum officinarum L.) genotypes

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Abstract

Increasing sugar content is the primary objective of sugarcane (Saccharum officinarum L.) improvement programs. To assess the importance of sucrose metabolizing enzymes in relation to sucrose content, enzymatic profiles of sucrose phosphate synthase (SPS), sucrose synthase (SuSy), acid and neutral invertases (AI and NI) were analyzed in relation to total sugars and reducing sugars in different sugarcane genotypes at 300, 360 and 420 days after planting (DAP). Varying responses in enzymatic profiles in relation to sugar content were noticed. The activities of SPS exceeded that of SuSy, AI and NI at 360 DAP, while SuSy was dominant at 420 DAP. Total sugars content were highest at 360 DAP and then declined at 420 DAP. Reducing sugars increased gradually from 300 to 420 DAP. Significant negative and positive correlations of SPS with reducing sugars and total sugars, respectively were noticed at 360 DAP. Interestingly, NI was strongly associated with reducing sugars, total sugars and ratio of total sugars to reducing sugars at 360 DAP as compared to AI. Differences between SPS–AI and SPS–NI exhibited significant negative correlation with reducing sugars and positive correlation with total sugars at 360 DAP. Differences between SPS–NI explained most of the variations found for sucrose accumulation in sugarcane. Our data demonstrate that synthesis and breakdown of sugars in sugarcane tissue is coordinated by the activities of AI, NI and SPS, respectively.

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Acknowledgments

Authors are grateful for the grant from Indian Council for Agricultural Research for the project. We thank Malegaon SSK factory and Vasantdada Sugar Institute, Pune for providing the sugarcane genotypes and laboratory facilities. Authors are also thankful to the Director and managing committee, Vasantdata Sugar Institute for funding this project. Authors would like to extend their thanks to the Director, The Deccan Sugar Technologies Association, India, for providing infrastructural facilities required for the work.

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  1. Sagar Datir

    Present address: Postgraduate Research Centre, Department of Botany, Modern College of Arts, Science and Commerce, Shivajinagar, Pune, 411005, India

Authors and Affiliations

  1. Department of Plant Physiology, Agricultural Sciences and Technology Division, Vasantdada Sugar Institute, Manjari, Pune, M.S., 412 307, India

    Sagar Datir & Snehal Joshi

  2. The Deccan Sugarcane Technologies Association, Shivajinagar, Pune, 411005, India

    Snehal Joshi

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  1. Sagar Datir
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Correspondence to Sagar Datir.

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Datir, S., Joshi, S. The contribution of sucrose metabolism enzymes to sucrose accumulation in sugarcane (Saccharum officinarum L.) genotypes. Ind J Plant Physiol. 21, 76–82 (2016). https://doi.org/10.1007/s40502-016-0205-8

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  • DOI: https://doi.org/10.1007/s40502-016-0205-8

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