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In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

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Abstract

Justicia gendarussa is a valuable medicinal plant and various parts of this plant are pharmaceutically used for the treatment of different diseases. In vitro regeneration of shoot buds was obtained from culture of nodal cuttings as well as shoot regeneration from callus. The nodal cuttings differed in shoot proliferation in terms of percentage of explants that responded and average shoot length with various concentrations (4.4, 8.9, 13.3, 17.7, 22.2 μM) of 6-benzyladenine (BA), kinetin (Kn) and thidiazuron. In all treatments, one shoot was invariably present. Optimum 87% of cultures responded with an average shoot length of 4.4 cm on Murashige and Skoog (MS) medium supplemented with 17.7 μM BA. Callus was induced from the mature leaf segments on MS medium supplemented with Kn (4.7, 13.9, 23.2 μM) alone or in combination with 2, 4-dichlorophenoxyacetic acid (2, 4-D; 2.3 μM, 4.5 μM). Optimum callus induction (78%) was obtained on MS medium supplemented with 14 μM Kn and 4.5 μM 2, 4-D. When the callus was subcultured on MS medium fortified with BA (8.9, 17.7, 26.6 μM) or Kn (9.3, 18.6, 27.9 μM) alone or in combination with α naphthalene acetic acid (NAA; 2.7, 5.4 μM), shoot regeneration was obtained. The highest response (92%) was observed on MS medium containing 17.7 μM BA and 5.4 μM NAA. On this medium, an average number of 12.2 shoots were obtained per responding callus. The shoots obtained from callus and nodal cuttings were rooted with a frequency of 73% on MS medium augmented with 9.8 μM indole-3-butyric acid. The rooted shoots were successfully transplanted to soil and sand mixture (1:1) with 90% survival rate. The protocol standardized for shoot proliferation and regeneration in J. gendarussa from nodal cuttings and leaf-derived callus is suitable for micropropagation and conservation of this essential medicinal plant.

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Abbreviations

BA:

6-Benzyladenine

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

Kn:

Kinetin

MS:

Murashige and Skoog

NAA:

α Naphthalene acetic acid

TDZ:

Thidiazuron

References

  • Aasim M, Khawar KM, Ozcan S (2009) In vitro micropropagation from plumular apices of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz. Sci Hort 122:468–471. doi:10.1016/j.scienta.2009.05.023

    Google Scholar 

  • Agastian P, Lincy W, Ignacimuthu S (2006) In vitro propagation of Justicia gendarussa Burm f.—a medicinal plant. Ind J Biotech 5:246–248

    Google Scholar 

  • Ahmad FB, Holdworth DK (2003) Medicinal plants of Sabah, East Malaysia—part 1. Pharm Biol 41:340–346

    Article  Google Scholar 

  • Anonymous (1959) The Wealth of India. CSIR Publications, New Delhi, ISBN: 81-85038-00-7, pp 312

  • Apers S, Vlietinck A, Pieters L (2003) Lignans and neolignans as lead compounds. Phytochem Rev 2:201–217

    Article  CAS  Google Scholar 

  • Balaraju K, Agastian P, Ignacimuthu S (2009) Micropropagation of Swertia chirata Buch.-Hams. ex Wall.: a critically endangered medicinal herb. Acta Physiol Plant 31:487–494. doi:10.1007/s11738-008-0257-0

    Article  Google Scholar 

  • Baskaran P, Jayabalan N (2008) In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture. Acta Physiol Plant 30:345–351. doi:10.1007/s11738-009-0330-3 (in press)

    Google Scholar 

  • Baskaran P, Velayutham P, Jayabalan N (2009) In vitro regeneration of Melothria maderaspatana via indirect organogenesis. In Vitro Cell Dev Biol Plant 45:407–413. doi:10.1007/s11627-008-9172-8

  • Bettaieb T, Mhamdi M, Hajlaoui I (2008) Micropropagation of Nolina recurvata Hemsl.: b-Cyclodextrin effects on rooting. Sci Hort 117:366–368. doi:10.1016/j.scienta.2008.05.023

    Article  CAS  Google Scholar 

  • Chakravarty AK, Dastidar PPG, Pakrashi SC (1982) Simple aromatic amines from Justicia gendarussa 13C NMR Spectra of the bases and their analogues. Tetrahedron 38:1797–1802

    Article  CAS  Google Scholar 

  • Dhaka N, Kothari SL (2005) Micropropagation of Eclipta alba (L.) Hassk. An important medicinal plant. In Vitro Cell Dev Biol Plant 41:770–774. doi:10.1079/IVP2005684

    Article  Google Scholar 

  • Dhar U, Joshi M (2005) Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators. Plant Cell Rep 24:195–200. doi:10.1007/s00299-005-0932-1

    Article  CAS  PubMed  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple F test. Biometrics 11:1–42

    Article  Google Scholar 

  • Huang CL, Hsieh MT, Hsieh WC, Sagare AP, Tsay HS (2000) In vitro propagation of Limonium wrightii (HANCE) KTZE. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf and inflorescence node explants. In Vitro Cell Dev Biol Plant 36:220–224

    Article  CAS  Google Scholar 

  • Jayaweera DMA (1980). Medicinal plants used in Ceylon, part 1 (National Science Council of Sri Lanka, Colombo) pp 16–17

  • Jonville MC, Kodja H, Humeau L, Fournel J, De Mol P, Cao M, Angenot L, Frédérich M (2008) Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity. J Ethnopharm 120:382–386. doi:10.1016/j.jep.2008.09.005

    Article  CAS  Google Scholar 

  • Kartsonas E, Papafotiou M (2007) Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tissue Organ Cult 90:111–116. doi:10.1007/s11240-007-9232-5

    Article  CAS  Google Scholar 

  • Kim YD, Min JY, Kim WJ, Kang YM, Moon HS, Lee CH, Prasad DT, Choi MS (2008) High frequency plant regeneration and accumulation of tropane alkaloids in regenerated plants of Scopolia parviflora. In Vitro Cell Dev Biol Plant 44:203–208. doi:10.1007/s11627-007-9073-2

    Article  CAS  Google Scholar 

  • Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79–83

    Article  CAS  Google Scholar 

  • Krishna KL, Mruthunjaya K, Patel JA (2009) Antioxidant and hepatoprotective activity of leaf extract of Justicia gendarussa Burm. Int J Biol Chem 3:99–110

    Article  CAS  Google Scholar 

  • Li Y, Gao J, Fei S (2009) High frequency embryogenic callus induction and plant regeneration from mature caryopsis of big bluestem and little bluestem. Sci Hort 121:348–352. doi:10.1016/j.scienta.2009.02.002

    Article  Google Scholar 

  • Majada JP, Sanchez-Tames R, Revilla MA, Casares A (2000) Micropropagation of Ilex aquifolium L. In Vitro Cell Dev Biol Plant 36:521–526

    Article  CAS  Google Scholar 

  • Makunga NP, Jager AK, van Staden J (2005) An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis—influence of auxins and cytokinins. Plant Cell Tissue Organ Cult 82:271–280. doi:10.1007/s11240-005-1372-x

    Article  CAS  Google Scholar 

  • Martin KP (2004) Plant regeneration protocol of medicinally important Andrographis paniculata (Burm. F.) Wallich Ex Nees via somatic embryogenesis. In Vitro Cell Dev Biol Plant 40:204–209. doi:10.1079/IVP2003520

    Article  Google Scholar 

  • Misic D, Grubisic D, Konjevic RK (2006) Micropropagation of Salvia brachyodon through nodal explants. Biol Plant 50:473–476

    Article  Google Scholar 

  • Mrunthunjaya K, Hukkeri VI (2007) Antioxidant and free radical scavenging potential of Justicia gendarussa Burm leaves in vitro. Nat Prod Sci 3:199–206

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nath S, Buragohain AK (2005) Micropropagation of Adhatoda vasica—a woody medicinal plant by shoot tip culture. Ind J Biotech 4:396–399

    Google Scholar 

  • Pattnaik S, Pradhan C, Naik SK, Chand PK (2000) Shoot organogenesis and plantlet regeneration from hypocotyl-derived cell suspensions of a tree legume Dalbergia sissoo Roxb. In Vitro Cell Dev Biol Plant 36:407–411

    Article  CAS  Google Scholar 

  • Perez-Bermudez P, Seitz HU, Gavidia I (2002) A protocol for rapid micropropagation of endangered Isoplexis. In Vitro Cell Dev Biol Plant 38:178–182. doi:10.1079/IVP2001282

    CAS  Google Scholar 

  • Periyanayagam K, Umamaheswari B, Suseela L, Padmini M, Ismail M (2009) Evaluation of antiangiogenic effect of the leaves of Justicia gendarussa (Burm. f) (Acanthaceae) by chrio allontoic membrane method. Am J Inf Dis 5:187–189

    Google Scholar 

  • Rani G, Grover IS (1999) In vitro callus induction and regeneration studies in Withania somnifera. Plant Cell Tissue Organ Cult 57:23–27

    Article  Google Scholar 

  • Ratnasooriya WD, Deraniyagala SA, Dehigaspitiya DC (2007) Antinociceptive activity and toxicological study of aqueous leaf extract of Justicia gendarussa Burm F. in rats. Phcog Mag 3:145–155

    Google Scholar 

  • Rout GR (2005) Micropropagation of Clitoria ternatea Linn. (Fabaceae)—an important medicinal plant. In Vitro Cell Dev Biol Plant 41:516–519. doi:10.1079/IVP2005675

    Article  Google Scholar 

  • Senthilkumar N, Varma P, Gurusubramanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector Anopheles stephensi (Liston). Parasitol Res 104:237–244. doi:10.1007/s00436-008-1180-4

    Article  CAS  PubMed  Google Scholar 

  • Singh SK, Rai MK, Asthana P, Pandey S, Jaiswal VS, Jaiswal U (2009) Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species. Acta Physiol Plant 31:649–653. doi:10.1007/s11738-008-0250-7

    Article  CAS  Google Scholar 

  • Thakur RC, Karnosky DF (2007) Micropropagation and germplasm conservation of Central Park Splendor Chinese elm (Ulmus parvifolia Jacq. ‘A/Ross Central Park’) trees. Plant Cell Rep 26:1171–1177. doi:10.1007/s00299-007-0334-7

    Article  CAS  PubMed  Google Scholar 

  • Thomas TD, Shankar S (2009) Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant. Plant Biotech Rep 3:67–74. doi:10.1007/s11816-008-0076-1

    Article  Google Scholar 

  • Tsay HS, Lee CY, Agrawal DC, Basker SG (2006) Influence of ventilation closure, gelling agent and explant type on shoot bud proliferation and hyperhydricity in Scrophularia yoshimurae—a medicinal plant. In Vitro Cell Dev Biol Plant 42:445–449. doi:10.1079/IVP2006791

    Article  CAS  Google Scholar 

  • Turker AU, Camper ND, Gurel E (2001) In vitro culture of common mullein (Verbascum thapsus L.). In Vitro Cell Dev Biol Plant 37:40–43. doi:10.1079/IVP2000111

    Article  CAS  Google Scholar 

  • Uawonggul N, Chaveerach A, Thammasirirak S, Arkaravichien T, Chuachan C, Daduang S (2006) Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis. J Ethnopharm 103:201–207. doi:10.1016/j.jep.2005.08.003

    Article  Google Scholar 

  • Woradulayapinij W, Soonthornchareonnon N, Wiwat C (2005) In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes. J Ethnopham 101:84–89. doi:10.1016/j.jep.2005.03.030

    Article  Google Scholar 

  • Yang LL, Yen KY, Kiso Y, Hikino H (1987) Antihepatotoxic actions of Formosan plant drugs. J Ethnopharm 19:103–110

    Article  CAS  Google Scholar 

  • Zheng X, Xing F (2009) Ethnobotanical study on medicinal plants around Mt. Yinggeling, Hainan Island, China. J Ethnopharm 124:197–210. doi:10.1016/j.jep.2009.04.042

    Article  Google Scholar 

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Acknowledgments

TDT acknowledges the financial assistance from the Indian National Science Academy (INSA) and Japan Society for the Promotion of Science (JSPS) in the form of a bilateral exchange programme (No. IA/JSPS/2009-2010).

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  1. T. Dennis Thomas

    Present address: Post Graduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram (P.O.), Kottayam, Kerala, 686574, India

Authors and Affiliations

  1. Field Science Centre for Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kita-ku, Sapporo, 060-0811, Japan

    T. Dennis Thomas & Hoshino Yoichiro

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Correspondence to T. Dennis Thomas.

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Communicated by E. Lojkowska.

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Dennis Thomas, T., Yoichiro, H. In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus. Acta Physiol Plant 32, 943–950 (2010). https://doi.org/10.1007/s11738-010-0482-1

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  • DOI: https://doi.org/10.1007/s11738-010-0482-1

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