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Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis (Lour) Olsson in vitro

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Summary

The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine in the explant decreased sharply after 1–3 days of culture, whereas endogenous spermine increased, irrespective of the absence or presence of Els. AgNO3 at 30 μM did not affect endogenous PAs during two weeks of culture. In contrast, explants grown on medium containing 5 μM AVG produced higher levels of free putrescine and spermine which increased rapidly after three days and reached a peak at 10 days. An exogenous application of 5 mM putrescine also resulted in a similar surge of endogenous free spermine of the explant. More strikingly, shoot regeneration from explants grown in the presence of 1–20 mM putrescine, 0.1–2.5 mM spermidine, or 0.1–1 mM spermine was enhanced after three weeks of culture. However, exogenous PAs generally did not affect ethylene production, and endogenous levels of 1-aminocyclopropane-1-carboxylate (ACC) synthase activity and ACC of the explant. This study shows the PA requirement for shoot regeneration from cotyledons of B. campestris ssp. pekinensis in vitro, and also indicates that the promotive effect of PAs on regeneration may not be due to an inhibition of ethylene biosynthesis.

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Abbreviations

PAs:

polyamines

AVG:

aminoethoxyvinylglycine

SAM:

S-adenosylmethionine

ACC:

1-aminocyclopropane-1-carboxylate

Els:

ethylene inhibitors

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Author notes

  1. Gek-Lan Chi

    Present address: Department of Biotechnology, Ngee Ann Polytechnic, 535 Clementi Road, 2159, Singapore, Republic of Singapore

  2. Justin E. E. Lee

    Present address: Institute of Plant Biochemistry, Weinberg 3, P.O. Box 250, D-06120, Halle, Germany

  3. Eng-Chong Pua

    Present address: Department of Botany, Faculty of Science, National University of Singapore, Lower Kent Ridge Road, 0511, Singapore, Republic of Singapore

Authors and Affiliations

  1. Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore, Republic of Singapore

    Gek-Lan Chi, Wen-Shu Lin, Justin E. E. Lee & Eng-Chong Pua

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  1. Gek-Lan Chi
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  2. Wen-Shu Lin
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  3. Justin E. E. Lee
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Communicated by J. J. Finer

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Chi, GL., Lin, WS., Lee, J.E.E. et al. Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis (Lour) Olsson in vitro . Plant Cell Reports 13, 323–329 (1994). https://doi.org/10.1007/BF00232630

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  • DOI: https://doi.org/10.1007/BF00232630

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