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Extraction of antioxidants and flavonoids from yuzu (Citrus junos Sieb ex Tanaka) peels: a response surface methodology study

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Abstract

In the present investigation, extraction of antioxidants and flavonoids from the peels of yuzu fruit using a single factor experiment and a response surface methodology (RSM) based on central composite design was studied. Four independent variables were evaluated at five levels with total 29 experimental runs, including ethanol concentration (EtOH), ratio of liquid to material (L/S), extraction temperature (T), and extraction time (t). The total phenolic content (TPC), total flavonoids content (TFC), two indicators of antioxidant capacity (FRAP and DPPH), and three individual major flavonoids in yuzu (hesperidin, naringin, and phloretin) served as the response functions. Quadratic polynomial equations were obtained by multiple regression analysis to predict the optimal extraction conditions. The regression analysis showed that >95 % of variations were explained by the models of different responses considered. The responses were significantly influenced by all studied factors. The Multiresponse optimized conditions targeted at maximizing all the responses were found to be EtOH = 65.550 %; T = 43.864 °C; t = 119.673 min; and L/S = 37.168 ml/g, with a desirability of 0.950. At the optimized conditions, the experimental values of FRAP (964.9 ± 23.1 mgTE/g DW), DPPH (453.0 ± 5.2 mgTE/g DW), TPC (1161.2 ± 25.2 mgGAE/g DW), (TFC393.4 ± mgQE/g DW), hesperidin (337.2 ± 4.0 mg/g DW), naringin (244.9 ± 1.1 mg/g DW), and phloretin (43.9 mg/g DW) were in a reasonable agreement with the predicted values. The extraction method was applied successfully to extract antioxidants and flavonoids from yuzu peels. It also allows a fast and cost-saving process for extraction of the studied phytochemicals, in addition to improvement of the quantity of the targeted extract.

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References

  1. K. Yoo, C.H. Lee, I. Hwang, Korean J. Food Cook. Sci. 22–227 (2008)

  2. K. Yoo, I. Hwang, Korean J. Food Sci. Technol. 36, 339 (2004)

    Google Scholar 

  3. S.H. Kim, H.J. Hur, H.J. Yang, H.J. Kim, M.J. Kim, J.H. Park, M.J. Sung, M.S. Kim, D.Y. Kwon, J.T. Hwang, Evid. Based Complement Altern. Med. 2013, (2013)

  4. P. Goetz, Phytothérapie, 12, 125 (2014)

    Article  Google Scholar 

  5. H.Y. Yu, S.W. Park, I.M. Chung, Y.S. Jung, Food Chem. Toxicol. 49, 3018 (2011)

    Article  CAS  Google Scholar 

  6. R. Hirota, N.N. Roger, H. Nakamura, H.S. Song, M. Sawamura, N. Suganuma, J. Food Sci. 75, 87–92 (2010)

  7. D.G. Lim, J.J. Kim, K.S. Seo, K.C. Nam, 39, 243 (2012)

  8. S. Lee, J. Shin, N. Sung, J. Agric. Life Sci. 44, 81 (2010)

    Google Scholar 

  9. K.M. Yoo, K.W. Lee, J.B. Park, H.J. Lee, I.K. Hwang, J. Agric. Food Chem. 52, 5907 (2004)

    Article  CAS  Google Scholar 

  10. K. Samarakoon, M. Senevirathne, W.W. Lee, Y.T. Kim, J. Il Kim, M.C. Oh, Y.J. Jeon, Nutr. Res. Pract. 6, 187 (2012)

    Article  CAS  Google Scholar 

  11. N. Miyazawa, N. Tomita, Y. Kurobayashi, A. Nakanishi, Y. Ohkubo, T. Maeda, A. Fujita, J. Agric. Food Chem. 57, 1990 (2009)

    Article  CAS  Google Scholar 

  12. N.T. Lan-Phi, T. Shimamura, H. Ukeda, M. Sawamura, Food Chem. 115, 1042 (2009)

    Article  Google Scholar 

  13. B. Tao, F. Ye, H. Li, Q. Hu, S. Xue, G. Zhao, J. Agric. Food Chem. 62, 7166 (2014)

  14. K.M. Yoo, I.K. Hwang, J.H. Park, B.K. Moon, J. Food Sci. 74, 462 (2009)

    Article  Google Scholar 

  15. T.W. Kim, K.K. Kang, Y.H. Kim, D.J. Choe, J. Nutr. Heal. 47, 221 (2014)

    Article  CAS  Google Scholar 

  16. O.C. Kwon, J. Korean Soc. Food Sci. Nutr. 35, 294 (2006)

    Article  Google Scholar 

  17. T. Suetsugu, H. Iwai, M. Tanaka, M. Hoshino, A. Quitain, M. Sasaki, M. Goto, Chem. Eng. Sci. 1, 87 (2013)

    Article  Google Scholar 

  18. D. Baş, İ.H. Boyacı, J. Food Eng. 78, 836 (2007)

    Article  Google Scholar 

  19. Q. Xu, Y. Shen, H. Wang, N. Zhang, S. Xu, L. Zhang, Food Chem. 138, 2122 (2013)

    Article  CAS  Google Scholar 

  20. B. Singh, H.K. Sharma, B.C. Sarkar, J. Food Sci. Technol. 49, 294 (2012)

    Article  CAS  Google Scholar 

  21. K.M. Hammi, A. Jdey, C. Abdelly, H. Majdoub, R. Ksouri, Food Chem. 184, 80 (2015)

    Article  CAS  Google Scholar 

  22. R. Tabaraki, E. Heidarizadi, A. Benvidi, Sep. Purif. Technol. 98, 16 (2012)

    Article  CAS  Google Scholar 

  23. W. Xiao, L. Han, B. Shi, Sep. Sci. Technol. 43, 671 (2008)

    Article  CAS  Google Scholar 

  24. Z.L. Sheng, P.F. Wan, C.L. Dong, Y.H. Li, Ind. Crops Prod. 43, 778 (2013)

    Article  CAS  Google Scholar 

  25. M.A. Al-Farsi, C.Y. Lee, Food Chem. 108, 977 (2008)

    Article  CAS  Google Scholar 

  26. C.M. Librán, L. Mayor, E.M. Garcia-Castello, D. Vidal-Brotons, Agric. Sci. 04, 56 (2013)

    Google Scholar 

  27. M.K. Khan, M. Abert-Vian, A.-S. Fabiano-Tixier, O. Dangles, F. Chemat, Food Chem. 119, 851 (2010)

    Article  CAS  Google Scholar 

  28. S. Kim, L. Asnin, A.D. Assefa, E.Y. Ko, K. Sharma, S.W. Park, Food Anal. Methods 7, 1804 (2014)

    Article  Google Scholar 

  29. J. Dai, R.J. Mumper, Molecules 15, 7313 (2010)

    Article  CAS  Google Scholar 

  30. V.L. Singleton, J.A. Rossi, Am. J. Enol. Vitic 16, 144 (1965)

    CAS  Google Scholar 

  31. C.C. Chang, M.H. Yang, H.M. Wen, J.C. Chern, J. Food Drug Anal. 10, 178 (2002)

    CAS  Google Scholar 

  32. I.F. Benzie, J.J. Strain, Anal. Biochem. 239, 70 (1996)

    Article  CAS  Google Scholar 

  33. W. Brand-Williams, M.E. Cuvelier, C. Berset, LWT Food Sci. Technol. 28, 25 (1995)

    Article  CAS  Google Scholar 

  34. L. Yang, Y.L. Cao, J.G. Jiang, Q.S. Lin, J. Chen, L. Zhu, J. Sep. Sci. 33, 1349 (2010)

    CAS  Google Scholar 

  35. B. Sultana, F. Anwar, M. Ashraf, Molecules 14, 2167 (2009)

    Article  CAS  Google Scholar 

  36. F. Kubota, Y. Baba, M. Goto, Solvent Extr. Res. Dev. Japan 19, 17 (2012)

    Article  CAS  Google Scholar 

  37. W. Liu, Y. Yu, R. Yang, C. Wan, B. Xu, S. Cao, Int. J. Mol. Sci. 11, 4750 (2010)

    Article  CAS  Google Scholar 

  38. K. Robards, Strategies for the determination of bioactive phenols in plants, fruit and vegetables. J. Chromatogr. A 1000, 657–691 (2003)

    Article  CAS  Google Scholar 

  39. G. Spigno, L. Tramelli, D.M. De Faveri, J. Food Eng. 81, 200 (2007)

    Article  CAS  Google Scholar 

  40. Y. Nagata, K.H. Chu, Biotechnol. Lett. 25, 1837 (2003)

    Article  CAS  Google Scholar 

  41. X. Guo, X. Zou, M. Sun, Carbohydr. Polym. 80, 344 (2010)

    Article  CAS  Google Scholar 

  42. a. Moure, J.M. Cruz, D. Franco, J. Manuel Domínguez, J. Sineiro, H. Domínguez, M.J. Núñez, J. Carlos Parajó, Food Chem. 72, 145 (2001)

    Article  CAS  Google Scholar 

  43. M. Markom, M. Hasan, W. Daud, H. Singh, J. Jahim, Sep. Purif. Technol. 52, 487 (2007)

    Article  CAS  Google Scholar 

  44. G.-Q. He, H.-P. Xiong, Q.-H. Chen, H. Ruan, Z.-Y. Wang, L. Traoré, J. Zhejiang Univ. Sci. B 6, 999 (2005)

    Article  Google Scholar 

  45. K. N. Prasad, E. Yang, C. Yi, M. Zhao, Y. Jiang, Innov. Food Sci. Emerg. Technol. 10, 155 (2009)

    Article  CAS  Google Scholar 

  46. F.I. Kanaze, E. Kokkalou, M. Georgarakis, I. Niopas, J. Pharm. Biomed. Anal. 36, 175 (2004)

    Article  CAS  Google Scholar 

  47. D.C. Abeysinghe, X. Li, C. Sun, W. Zhang, C. Zhou, K. Chen, Food Chem. 104, 1338 (2007)

    Article  CAS  Google Scholar 

  48. V. Goulas, G.A. Manganaris, Food Chem. 131, 39 (2012)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Konkuk University, Seoul, Republic of Korea

    Awraris Derbie Assefa, Ramesh Kumar Saini & Young Soo Keum

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  1. Awraris Derbie Assefa
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  2. Ramesh Kumar Saini
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Correspondence to Young Soo Keum.

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Assefa, A.D., Saini, R.K. & Keum, Y.S. Extraction of antioxidants and flavonoids from yuzu (Citrus junos Sieb ex Tanaka) peels: a response surface methodology study. Food Measure 11, 364–379 (2017). https://doi.org/10.1007/s11694-016-9405-1

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