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Optimization of extraction of antioxidants from wheat bran (Triticum spp.) using response surface methodology

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Abstract

In this study, the optimal conditions for the extraction of antioxidants from hard winter wheat bran (WH711) were determined using response surface methodology (RSM). A central composite face centred design was used to investigate the effects of three independent variables, namely solvent composition (%v/v), extraction temperature (°C) and time (minutes) on the responses; free phenolic content (FPC), total flavonoid content (TFC), total antioxidant activity (TAA), ferric reducing power (FRP), hydrogen peroxide scavenging activity (HPSA). Regression analysis showed that more than 90% of the variation was explained by the models of different responses. The optimal conditions for the antioxidantss extraction were found to be methanol concentration of 85%v/v, extraction temperature of 75 °C, extraction time of 45 min, for wheat bran. The experimental values of FPC, TFC, TAA, FRP and HPSA were 0.921 mg GAE/g bran (B), 0.4588 mg CE/g B, 0.01408 mM AAE/g B, 2.532 mM AAE/g B and 3.193 mM TE/g B, respectively which agreed with those predicted, thus indicating suitability of the model employed and the suitability of RSM in optimizing the extraction conditions.

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Authors and Affiliations

  1. Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology (Deemed To Be University), Longowal, Dist.-Sangrur, Punjab, India, 148106

    Barinderjit Singh, Harish Kumar Sharma & Bhavesh Chandra Sarkar

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  1. Barinderjit Singh
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  2. Harish Kumar Sharma
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  3. Bhavesh Chandra Sarkar
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Correspondence to Barinderjit Singh.

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Singh, B., Sharma, H.K. & Sarkar, B.C. Optimization of extraction of antioxidants from wheat bran (Triticum spp.) using response surface methodology. J Food Sci Technol 49, 294–308 (2012). https://doi.org/10.1007/s13197-011-0276-5

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  • DOI: https://doi.org/10.1007/s13197-011-0276-5

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