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Improved production of Oroxylum indicum (L.) Kurz by altering the salts of the medium

  • Plant Tissue Culture
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Abstract

The present study was conducted to test the effects of KNO3, KH2PO4, and CaCl2 on shoot multiplication, root proliferation, and accumulation of phytochemicals in in vitro cultures of Oroxylum indicum. The results indicate that modifying the MS salt formulation in relation to particular inorganic nutrients highly affected shoot multiplication, root proliferation, and accumulation of flavonoids in in vitro cultures. A concentration of 0.60 g L−1 CaCl2 resulted in the highest frequency of shoot regeneration (5.6 shoots per explant). A concentration of 0.40 g L−1 CaCl2 resulted in the highest frequency of root regeneration (7.8 roots per shoot). Modifications of the concentrations of inorganic salts were also found to be advantageous for production media for both multiple shoots and shoot-derived root in vitro cultures. Multiple shoots generated on shoot induction medium with a concentration of 0.60 g L−1 CaCl2 and roots generated on root induction medium with a concentration of 1.5 g L−1 KNO3 yielded about a five times higher flavonoid level than cultures generated on control medium respectively.

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References

  • Abu-Quamar SF, Sors TG, Cunningham SM, Joern BC, Volenec JJ (2005) Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells. Plant Cell Tissue Organ Cult 82:131–140

    Article  Google Scholar 

  • Ahuja MR (1993) Micropropagation of nitrogen-fixing trees. In: In Micropropagation of woody plants. Kluwer Academic Publishers, Dordretch, Netherlands, pp 303–315

    Chapter  Google Scholar 

  • Anonymous (1998) The Ayurvedic Pharmacopoeia of India, New Delhi, Government of India, Ministry of Health and Family Welfare. Depart Indian Syst Med Homeopathy 3:209–210

    Google Scholar 

  • Arruda SCC, Souza GM, Almeida M, Goncalues A (2000) Anatomical and biochemical characterization of the calcium effect on Eucalyptus urophylla callus morphogenesis in vitro. Plant Cell Tissue Organ Cult 63:142–154

    Article  Google Scholar 

  • Capitani F, Altamura MM (2004) Exogenous calcium enhances the formation of vegetative buds, flowers and roots in tobacco pith explants cultured in the absence of exogenous hormones. Plant Cell Tissue Organ Cult 77:1–10

    Article  CAS  Google Scholar 

  • Chattopadhyay S, Farkya S, Srivastava AK, Bisaria VS (2002) Bioprocess considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnol Bioprocess Eng 7:138–149

    Article  CAS  Google Scholar 

  • Chauhan M, Kothari SL (2005) Influence of potassium dihydrogen phosphate on callus induction and plant regeneration in rice (Oryza sativa L.) Cereal Res Commun 33:553–560

    Article  CAS  Google Scholar 

  • Christen P, Aoki T, Shimomura K (1992) Characteristics of growth and tropane alkaloid production in Hyoscyamus albus hairy roots transformed with Agrobacterium rhizogenes A4. Plant Cell Rep 11:597–600

    Article  CAS  PubMed  Google Scholar 

  • Dalal NV, Rai VR (2004) In vitro propagation of Oroxylum indicum Vent—a medicinally important forest tree. J For Res 9:61–65

    Article  CAS  Google Scholar 

  • Dalton CC, Iqball K, Turner DA (1983) Iron phosphate precipitation in Murashige and Skoog media. Physiol Plant 57:472–476

    Article  CAS  Google Scholar 

  • De-Eknamkul W, Ellis BE (1985) Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep 4:46–49

    Article  CAS  PubMed  Google Scholar 

  • Gokhale M, Bansal YK (2006) An avowal of importance of endangered tree Oroxylum indicum (Linn.) Vent. Nat Prod Radiance 5:112–114

    Google Scholar 

  • Gokhale M, Bansal YK (2009) Direct in vitro regeneration of a medicinal tree Oroxylum indicum (L.) Vent. Through tissue culture. Afr J Biotechnol 8:3777–3781

    CAS  Google Scholar 

  • Gokhale M, Bansal YK (2010) Assessment of secondary metabolites in in vitro regenerated plantlets of Oroxylum indicum (L.) Vent. Plant Tissue CultBiotechnol 20:21–28

    Google Scholar 

  • Haehnel W (1984) Photosynthetic electron transport in higher plants. Annu Rev Plant Physiol 35:659–693

    Article  CAS  Google Scholar 

  • Jansen MAK, Booij H, Schel JHN, deVries SC (1990) Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures. Plant Cell Rep 9:221–223

    Article  CAS  PubMed  Google Scholar 

  • Jayaram K, Prasad MNV (2008) Genetic diversity in Oroxylum indicum (L) Vent (Bignoniaceae), a vulnerable medicinal plant by random amplified polymorphic DNA marker. Afr J Biotechnol 7:254–262

    CAS  Google Scholar 

  • Lin JY, Tang CY (2007) Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. FoodChem 101:140–147

    CAS  Google Scholar 

  • Mahalakshmi A, Singla B, Khurana JP, Khurana P (2007) Role of calcium-calmodulin in auxin-induced somatic embryogenesis in leaf base cultures of wheat (Triticum aestivum var. HD 2329). Plant Cell Tissue Organ Cult 88:167–174

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Parmar VR, Jasrai YT (2014) Effect of cytokinin and auxin on micropropagation of Oroxylum indicum (L.) Vent: a “mountain tree of India”. Int J Life Sci Res 2:58–64

    Google Scholar 

  • Ramage CM, Williams RR (2002) Mineral nutrition and plant morphogenesis. In Vitro Cell Dev Biol-Plant 38(2):116–124

  • Raven JA, Smith FA (1976) Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation. New Phytol 76:415–431

    Article  CAS  Google Scholar 

  • Sarkar D, Pandey SK, Chanemougasoundharam A, Sud KC (2005) The role of calcium nutrition in potato (Solanum tuberosum) microplants in relation to minimal growth over prolonged storage in vitro. Plant Cell Tissue Organ Cult 81:221–227

    Article  Google Scholar 

  • Sinha A (2008) In vitro morphogenetic studies in Withania somnifera (L) Dunal, Ph. D. thesis. University of Rajasthan, Jaipur, India

    Google Scholar 

  • Subramanian SS, Nair AGR (1972) Flavanoids of the leaves of Oroxylum indicum and Pajanelia longifolia. Phytochemistry 11:439

    Article  CAS  Google Scholar 

  • Taiz L, Zaiger E (1991) Mineral nutrition. Plant Physiol 2:103–124

  • Tiwari S, Singh K, Shah P (2007) In vitro propagation of Oroxylum—an endangered medicinal tree. Biotechnology 6:299–301

    Article  CAS  Google Scholar 

  • Tripathi L, Tripathi JN (2003) Role of biotechnology in medicinal plants. Trop J Pharm Res 2:243–253

    Google Scholar 

  • Yamamoto H, Chatani N, Kitayama A, Tomimori T (1986) Flavonoid production in Scutellaria baicalensis callus cultures. Plant Cell Tissue Organ Cult 5:219–222

    Article  CAS  Google Scholar 

  • Yu KW, Gao WY, Hahn EJ, Paek KY (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng CA Meyer). J Plant Biol 44:179–184

    Article  CAS  Google Scholar 

  • Zaveri M, Khandhar A, Jain S (2008) Quantification of baicalein, chrysin, biochanin-A and ellagic acid in root bark of Oroxylum indicum by RP-HPLC with UV detection. Eurasian J Anal Chem 3:245–257

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, University School of Sciences, Gujarat University, Ahmedabad, -380009, India

    Vilas R. Parmar & Yogesh T. Jasrai

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  1. Vilas R. Parmar
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  2. Yogesh T. Jasrai
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Correspondence to Vilas R. Parmar.

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Editor: Wenhao Dai

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Parmar, V.R., Jasrai, Y.T. Improved production of Oroxylum indicum (L.) Kurz by altering the salts of the medium. In Vitro Cell.Dev.Biol.-Plant 54, 79–86 (2018). https://doi.org/10.1007/s11627-017-9863-0

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  • DOI: https://doi.org/10.1007/s11627-017-9863-0

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