Skip to main content
SpringerLink
Log in

Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds

  • Organogenesis
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Regeneration of sunflower plants from tissue culture can be problematic due to inefficiencies in shoot elongation and subsequent rooting. To minimize exposure to cytokinins, which can negatively impact shoot elongation and rooting, a cytokinin pulse treatment was evaluated by placing cotyledonary tissues from sunflower seeds onto a shoot induction medium containing 1.5 mg L−1 6-benzylaminopurine and 0.2 mg L−1 1-naphthaleneacetic acid for 0, 2, 4, 8, 12, and 16 d, prior to transfer to an elongation medium containing 0.1 mg L−1 gibberellic acid. The 16-d pulse treatment gave the highest numbers of shoot primordia, but shoot development was minimal and only 0.4 well-developed shoots could be obtained per explant. The 4-d pulse treatment followed by 14 d of culture on elongation medium led to the production of 5.5 well-developed shoots per explant. For plant recovery, well-developed shoots obtained with the 4-d pulse treatment followed by a 14-d elongation treatment were micrografted onto in vitro seedlings, with graft survival of over 60%. Short pulse treatments (4- and 8-d), followed by culture on elongation medium, generated well-developed shoots that seemed to be more responsive to micrografting than shoots obtained with the 16-d pulse treatment. The use of the 4-d pulse treatment with micrografting of well-developed shoots significantly improved the efficiency of whole-plant recovery of sunflower.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  • Abousalim A, Mantell SH (1992) Micrografting of pistachio (Pistacia vera L. cv. Mateur). Plant Cell Tissue Organ Cult 29:231–234

    Article  Google Scholar 

  • Auer CA, Motyka V, Březinová A, Kamínek M (1999) Endogenous cytokinin accumulation and cytokinin oxidase activity during shoot organogenesis of Petunia hybrida. Physiol Plant 105:141–147

    Article  CAS  Google Scholar 

  • Baker C, Munoz-Fernandez N, Carter C (1999) Improved shoot development and rooting from mature cotyledons of sunflower. Plant Cell Tissue Organ Cult 58:39–49

    Article  CAS  Google Scholar 

  • Bellini C, Pacurar DI, Perrone I (2014) Adventitious roots and lateral roots: similarities and differences. Annu Rev Plant Biol 65:639–666

    Article  CAS  PubMed  Google Scholar 

  • Bronner R, Jeannin G, Hahne G (1994) Early cellular events during organogenesis and somatic embryogenesis induced on immature zygotic embryos of sunflower (Helianthus annuus). Can J Bot 72:239–248

    Article  Google Scholar 

  • Burrus M, Molinier J, Himber C, Hunold R, Bronner R, Rousselin P, Hahne G (1996) Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns. Mol Breed 2:329–338

    Article  CAS  Google Scholar 

  • Ceriani MF, Hopp HE, Hahne G, Escandón AS (1992) Cotyledons: an explant for routine regeneration of sunflower plants. Plant Cell Physiol 33:157–164

    Google Scholar 

  • Charrière F, Sotta B, Miginiac É, Hahne G (1999) Induction of adventitious shoots or somatic embryos on in vitro cultured zygotic embryos of Helianthus annuus: variation of endogenous hormone levels. Plant Physiol Biochem 37:751–757

    Article  Google Scholar 

  • Christianson M, Warnick D (1983) Competence and determination in the process of in vitro shoot organogenesis. Dev Biol 95:288–293

    Article  CAS  PubMed  Google Scholar 

  • Dhaka N, Kothari S (2002) Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Helianthus annuus L. Plant Cell Rep 21:29–34

    Article  CAS  Google Scholar 

  • Drake P, John A, Power J, Davey M (1997) Cytokinin pulse-mediated shoot organogenesis from cotyledons of Sitka spruce [Picea sitchensis (Bong.) Carr.] and high frequency in vitro rooting of shoots. Plant Cell Tissue Organ Cult 50:147–151

    Article  CAS  Google Scholar 

  • Duclercq J, Sangwan-Norreel B, Catterou M, Sangwan RS (2011) De novo shoot organogenesis: from art to science. Trends Plant Sci 16:597–606

    Article  CAS  PubMed  Google Scholar 

  • Finer JJ (1987) Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium. Plant Cell Rep 6:372–374

    Article  CAS  PubMed  Google Scholar 

  • Gamborg OL, Miller R, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  PubMed  Google Scholar 

  • Goldfarb B, Howe GT, Bailey LM, Strauss SH, Zaerr JB (1991) A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons. Plant Cell Rep 10:156–160

    CAS  PubMed  Google Scholar 

  • Gulati A, Schryer P, McHughen A (2001) Regeneration and micrografting of lentil shoots. In Vitro Cell Dev Biol Plant 37:798–802

    Article  CAS  Google Scholar 

  • Huang L, Huang B, Murashige T (1998) A micropropagation protocol for Cinnamomum camphora. In Vitro Cell Dev Biol Plant 34:141–146

    Article  Google Scholar 

  • Ivanova M, van Staden J (2008) Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla. Plant Cell Tissue Organ Cult 92:227–231

    Article  CAS  Google Scholar 

  • Jang J, Tainter F (1991) Micropropagation of shortleaf, Virginia and loblolly × shortleaf pine hybrids via organogenesis. Plant Cell Tissue Organ Cult 25:61–67

    Article  Google Scholar 

  • Jasinski S, Piazza P, Craft J, Hay A, Woolley L, Rieu I, Tsiantis M (2005) KNOX action in Arabidopsis is mediated by coordinate regulation of cytokinin and gibberellin activities. Curr Biol 15:1560–1565

    Article  CAS  PubMed  Google Scholar 

  • Kataeva NV, Alexandrova IG, Butenko RG, Dragavtceva EV (1991) Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro. Plant Cell Tissue Organ Cult 27:149–154

    Article  CAS  Google Scholar 

  • Knittel N, Escandón AS, Hahne G (1991) Plant regeneration at high frequency from mature sunflower cotyledons. Plant Sci 73:219–226

    Article  Google Scholar 

  • Konov A, Bronner R, Skryabin K, Hahne G (1998) Formation of epiphyllous buds in sunflower (Helianthus annuus L.): induction in vitro and cellular origin. Plant Sci 135:77–86

    Article  CAS  Google Scholar 

  • Lakshmanan P, Ng SK, Loh CS, Goh CJ (1997) Auxin, cytokinin and ethylene differentially regulate specific developmental states associated with shoot bud morphogenesis in leaf tissues of mangosteen (Garcinia mangostana L.) cultured in vitro. Plant Cell Physiol 38:59–64

    Article  CAS  Google Scholar 

  • Leshem B, Shaley D, Izhar S (1988) Cytokinin as an inducer of vitrification in melon. Ann Bot 61:255–260

    CAS  Google Scholar 

  • Luo J, Gould JH (1999) In vitro shoot-tip grafting improves recovery of cotton plants from culture. Plant Cell Tissue Organ Cult 57:211–213

    Article  Google Scholar 

  • Madhulatha P, Anbalagan M, Jayachandran S, Sakthivel N (2004) Influence of liquid pulse treatment with growth regulators on in vitro propagation of banana (Musa spp. AAA). Plant Cell Tissue Organ Cult 76:189–192

    Article  CAS  Google Scholar 

  • Mercier H, Souza BM, Kraus JE, Hamasaki RM, Sotta B (2003) Endogenous auxin and cytokinin contents associated with shoot formation in leaves of pineapple cultured in vitro. Braz J Plant Physiol 15:107–112

    Article  CAS  Google Scholar 

  • Mneney EE, Mantell SH (2001) In vitro micrografting of cashew. Plant Cell Tissue Organ Cult 66:49–58

    Article  Google Scholar 

  • Moncaleán P, Rodríguez A, Fernández B (2001) In vitro response of Actinidia deliciosa explants to different BA incubation periods. Plant Cell Tissue Organ Cult 67:257–266

    Article  Google Scholar 

  • Müller A, Iser M, Hess D (2001) Stable transformation of sunflower (Helianthus annuus L.) using a non-meristematic regeneration protocol and green fluorescent protein as a vital marker. Transgenic Res 10:435–444

    Article  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Navarro L, Roistacher C, Murashige T (1975) Improvement of shoot-tip grafting in vitro for virus-free citrus. J Am Soc Hortic Sci 100:471–479

    Google Scholar 

  • Paterson KE (1984) Shoot tip culture of Helianthus annuus—flowering and development of adventitious and multiple shoots. Am J Bot 71:925–931

    Article  Google Scholar 

  • Peña L, Cervera M, Juárez J, Navarro A, Pina JA, Durán-Vila N, Navarro L (1995a) Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. Plant Cell Rep 14:616–619

    Article  PubMed  Google Scholar 

  • Peña L, Cervera M, Juárez J, Ortega C, Pina J, Durán-Vila N, Navarro L (1995b) High efficiency Agrobacterium-mediated transformation and regeneration of citrus. Plant Sci 104:183–191

    Article  Google Scholar 

  • Polanco M, Ruiz M (1997) Effect of benzylaminopurine on in vitro and in vivo root development in lentil, Lens culinaris Medik. Plant Cell Rep 17:22–26

    Article  Google Scholar 

  • Power CJ (1987) Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos. Am J Bot 74:497–503

    Article  CAS  Google Scholar 

  • Pulido CM, Harry IS, Thorpe TA (1992) Optimization of bud induction in cotyledonary explants of Pinus canariensis. Plant Cell Tissue Organ Cult 29:247–255

    Article  Google Scholar 

  • Puthra G, Anil SR (2002) In vitro grafting of cashew (Anacardium occidentale L.). Sci Hortic 92:177–182

    Article  Google Scholar 

  • Sugiyama M (1999) Organogenesis in vitro. Curr Opin Plant Biol 2:61–64

    Article  CAS  PubMed  Google Scholar 

  • Sujatha M, Reddy T (1998) Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Rep 17:561–566

    Article  CAS  Google Scholar 

  • Sujatha M, Vijay S, Vasavi S, Sivaraj N, Rao SC (2012) Combination of thidiazuron and 2-isopentenyladenine promotes highly efficient adventitious shoot regeneration from cotyledons of mature sunflower (Helianthus annuus L.) seeds. Plant Cell Tissue Organ Cult 111:359–372

    Article  CAS  Google Scholar 

  • Wu H, du Toit ES, Reinhardt C (2007) Micrografting of Protea cynaroides. Plant Cell Tissue Organ Cult 89:23–28

    Article  Google Scholar 

  • Yamaguchi S (2008) Gibberellin metabolism and its regulation. Annu Rev Plant Biol 59:225–251

    Article  CAS  PubMed  Google Scholar 

  • Zhang Z, Finer JJ (2015) Sunflower (Helianthus annuus L.) organogenesis from primary leaves of young seedlings preconditioned by cytokinin. Plant Cell Tissue Organ Cult 123:645–655

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was funded by the US Department of Agriculture–Agricultural Research Service program National Sclerotinia Initiative and by state and federal funds appropriated to the Ohio State University/Ohio Agricultural Research and Development Center. Mention of trademark or proprietary products does not constitute a guarantee or warranty of the product by OSU/OARDC and also does not imply approval to the exclusion of other products that may also be suitable.

Author information

Author notes

  1. Zhifen Zhang

    Present address: Department of Horticulture, The University of Georgia Tifton Campus, Tifton, GA, 31973, USA

Authors and Affiliations

  1. Department of Horticulture and Crop Science, OARDC/The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA

    Zhifen Zhang & John J. Finer

Authors
  1. Zhifen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John J. Finer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John J. Finer.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Z., Finer, J.J. Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds. In Vitro Cell.Dev.Biol.-Plant 52, 391–399 (2016). https://doi.org/10.1007/s11627-016-9770-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-016-9770-9

Keywords

Search

Navigation