Abstract
A method is described for using liquid pulsing as an alternative to the conventional induction protocol for Pinus canariensis. Using Day 0 and Day 3 explants, the best exposure time was 8 h and 4 h respectively, in a non-buffered 100 μM N6-benzyladenine solution, followed by culture on half-strength Bornman's medium containing 3% sucrose and 0.8% Difco BactoR agar. With this procedure, 97% of the cotyledonary explants produced about 14 buds/explant. These results were comparable to a 14-day induction period on full-strength Bornman's medium containing 10 μM N6-benzyladenine.
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Pulido, C.M., Harry, I.S. & Thorpe, T.A. Optimization of bud induction in cotyledonary explants of Pinus canariensis . Plant Cell Tiss Organ Cult 29, 247–255 (1992). https://doi.org/10.1007/BF00034360
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DOI: https://doi.org/10.1007/BF00034360