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In vitro production of adventitious roots containing asiaticoside from leaf tissues of Centella asiatica L.

  • Developmental Biology
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Abstract

Centella asiatica (Apiaceae) is an important medicinal herb used in a variety of herbal medicines worldwide. Although the whole plant contains important triterpenoids, a significant quantity of pharmacologically important phytochemicals collectively known as centellosides can be extracted from leaf tissues and not from other parts of this plant. Asiaticoside is one of the major centellosides and is used in holistic medicine for treating a variety of human ailments. Genotypes of C. asiatica of Indian origin accumulate significant quantities of asiaticoside in their roots, while genotypes from other continents contain insignificant quantities of this chemical. The main purpose of this study was to manipulate the leaf-derived callus of C. asiatica using a combination of plant growth regulators to generate a large quantity of adventitious roots. The presence of asiaticoside in callus and regenerated roots of C. asiatica was detected by thin layer chromatography as well as by high-performance liquid chromatography, and the accumulation of a significant quantity of asiaticoside was demonstrated by spectrophotometric analysis. The protocol developed for the regeneration of roots was simple, reproducible, and reliable for the possible commercial production of root biomass enriched for asiaticoside.

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Acknowledgments

This research work was carried out by the first author (S. Mercy) for her PhD. Research facilities are partly provided under the project Collection, Conservation and Molecular Characterization of wild and hybrid derivatives of Amla (Phyllanthus emblica) germplasm in Tirunelveli District, Tamilnadu (no. 33-244/2007 (SR), dated 24 December 2007) by the University Grants Commission, Government of India, New Delhi, India. We thank Dr. O.T. Kim, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Emseong 369-873, Republic of Korea for generously providing standard asiaticoside for HPLC analysis.

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Authors and Affiliations

  1. Plant Genetic Improvement Laboratory, Department of Biotechnology, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, 627 412, Tirunelveli District, Tamil Nadu, India

    Steephen Mercy & Nagarajan Sangeetha

  2. Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, 625 021, Madurai, Tamil Nadu, India

    Doss Ganesh

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  1. Steephen Mercy
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  2. Nagarajan Sangeetha
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  3. Doss Ganesh
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Correspondence to Doss Ganesh.

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Editor: J. Finer

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Mercy, S., Sangeetha, N. & Ganesh, D. In vitro production of adventitious roots containing asiaticoside from leaf tissues of Centella asiatica L.. In Vitro Cell.Dev.Biol.-Plant 48, 200–207 (2012). https://doi.org/10.1007/s11627-011-9416-x

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