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Production of Biomass and Bioactive Compounds in Adventitious Root Cultures of Eleutherococcus koreanum Nakai

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Production of Biomass and Bioactive Compounds Using Bioreactor Technology

Abstract

Eleutherococcus koreanum Nakai is an endemic medicinal plant grown in Jeju Island, South Korea. Extracts from this plant have traditionally been used in Korea as a tonic and for treating rheumatism, diabetes and hepatitis. These extracts contain many useful bioactive substances, particularly eleutherosides, chlorogenic acid and other phenolic compounds. The quality and quantity of major bioactive compounds from naturally grown plants are greatly affected by harvest time and environmental conditions. Therefore, determination of suitable growing and harvesting conditions is necessary to achieve reliable supply of E. koreanum-based bioactive compounds for commercial use. To establish an efficient method for the year-round production of bioactive compounds, adventitious roots of E. koreanum were tested with various physical and chemical factors (inoculum density, aeration volume, salt strength, nitrogen source, and sucrose concentration) that affect root biomass and production of target bioactive compounds. Root biomass, concentrations of five target bioactive compounds (eleutherosides B and E, chlorogenic acid, total phenolics, and flavonoids), physiological responses of the adventitious roots and other environmental conditions in the culture vessels were determined. In addition, responses of roots subjected to chemical elicitors (methyl jasmonate and salicylic acid) were evaluated to determine a strategy for enhancing the final production of bioactive compounds. Finally, we compared the contents of bioactive compounds and typical DNA histograms for the adventitious roots and naturally grown plants to verify the competitive ability and genetic stability of cultured E. koreanum adventitious roots. The development of in vitro culture protocol, which controls the quality and quantity of elicited bioactive compounds, will be beneficial for the pilot-scale production of E. koreanum-based bioactive compounds for commercial use.

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Abbreviations

DPPH:

2,2-diphenyl-1-picrylhydrazyl

DW:

Dry weight

FW:

Fresh weight

H2O2 :

Hydrogen peroxide

HPLC:

High-performance liquid chromatography

IBA:

Indole-3-butyric acid

MJ:

Methyl jasmonate

MS:

Murashige and Skoog

PAL:

Phenylalanine ammonia lyase

ROS:

Reactive oxygen species

SA:

Salicylic acid

TDZ:

Thidiazuron; N-phenyl-N′-1,2,3,-thidiazol-5-ylurea

vvm:

Air volume culture volume−1 min−1

References

  1. Kang JY, Jeong YH, Lim EH, Lee EJ, Chu HM, Jeong SJ (2005) Analysis of acanthoic acid and isomers in Acanthopananx species by gas chromatography. J Pharm Sci 20:41–46

    Google Scholar 

  2. Slacanin I, Marston A, Hostettmann K (1991) The isolation of Eleutherococcus senticosus constituents by centrifugal partition chromatography and their quantitative determination by high performance liquid chromatography. Phytochem Anal 2:137–142

    Article  CAS  Google Scholar 

  3. Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S (1996) Protective effects of Acanthopanax senticosus Harms form Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol Pharm Bull 19:1227–1230

    Article  PubMed  CAS  Google Scholar 

  4. Lee EJ, Paek KY (2012) Effect of nitrogen source on biomass and bioactive compound production of in submerged cultures of Eleutherococcus koreanum Nakai adventitious roots. Biotechnol Prog 28:508–514

    Article  PubMed  CAS  Google Scholar 

  5. Ko HJ, Song CK, Cho NK (2003) Growth of seedling and germination characteristics of Acanthopanax koreanum Nakai. Kor J Med Crop Sci 11:46–52

    Google Scholar 

  6. Jwa CS, Yang YT, Koh JS (2000) Changes in free sugars, organic acids, free amino acids and minerals by harvest time and parts of Acanthopanax koreanum. J Kor Soc Agric Chem Biotechnol 43:106–109

    CAS  Google Scholar 

  7. Shohael AM, Murthy HN, Lee HL, Hahn EJ, Islam R, Paek KY (2008) Increased eleutheroside production in Eleutherococcus senticosus embryogenic suspension cultures with methyl jasmonate treatment. Biochem Eng J 38:270–273

    Article  CAS  Google Scholar 

  8. Baque MA, Moh SH, Lee EJ, Zhong ZZ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol Adv 30:1255–1267

    Article  PubMed  CAS  Google Scholar 

  9. Sivakumar G, Medina-Bolivar F, Lay JO, Dolan MC, Condori J, Grubbs SK, Wright SM, Baque MA, Lee EJ, Paek KY (2011) Bioprocess and bioreactor: next generation technology for production of potential plant-based antidiabetic and antioxidant molecules. Curr Med Chem 18:79–90

    Article  PubMed  CAS  Google Scholar 

  10. Wang J, Man S, Gao W, Zhang L, Huang L (2013) Cluster analysis of ginseng tissue cultures, dynamic change of growth, total saponins, and specific oxygen uptake rate in bioreactor and immuno-regulative effect of ginseng adventitious root. Ind Crops Prod 41:57–63

    Article  Google Scholar 

  11. Ahn JK, Park SY, Lee WY, Lee JJ (2005) Effects of growth regulators on adventitious root growth and eleutherosides and chlorogenic acid accumulation in air lift bioreactor cultures of Eleutherococcus koreanum. Kor J Plant Biotechnol 32:57–61

    Article  Google Scholar 

  12. Murashige T, Skoog F (1962) A revise medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  13. Lee EJ, Kim MK, Paek KY (2010) Auxin and cytokinin affect biomass and bioactive compound production from adventitious roots of Eleutherococcus koreanum. Kor J Hort Sci Technol 28:678–684

    Google Scholar 

  14. Lee EJ, Moh SH, Paek KY (2011) Influence of inoculum density and aeration volume on biomass and bioactive compound production in bulb-type bubble bioreactor cultures of Eleutherococcus koreanum Nakai. Bioresour Technol 102:7165–7170

    Article  PubMed  CAS  Google Scholar 

  15. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199

    Article  CAS  Google Scholar 

  16. Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scoplida parviflora adventitious roots. BioresourTechnol 98:1748–1753

    Article  CAS  Google Scholar 

  17. Fischer U, Alfermann AW (1995) Cultivation of photoautotrophic plant cell suspension in the bioreactor: influence of culture conditions. J Biotechnol 41:19–28

    Article  CAS  Google Scholar 

  18. Meijer JJ, Hoopen HJG, Libbenga KR (1993) Effects of hydrodynamic stress on cultured plant cell: a literature survey. Enz Microb Technol 15:234–238

    Article  CAS  Google Scholar 

  19. Jeong JA, Wu CH, Murthy HN, Hahn EJ, Paek KY (2009) Application of airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol Bioproc Eng 14:91–98

    Article  CAS  Google Scholar 

  20. Gao J, Lee JM (1992) Effect of oxygen supply on the suspension cultures of genetically modified tobacco cell. Biotechnol Prog 8:285–290

    Article  PubMed  CAS  Google Scholar 

  21. Schlatmann JE, Fonck E, ten Hoopen HJG, Heijhen JJ (1993) The negligible role of carbon dioxide and ethylene in ajmalicine production by Catharanthus roseus cell suspensions. Plant Cell Rep 14:157–160

    Google Scholar 

  22. Amirouche L, Stuchbury T, Matthews S (1985) Comparisons of cultivar performance on different nutrient media in a routine method for potato micropropagation. Potato Res 28:469–478

    Article  Google Scholar 

  23. Lee EJ, Paek KY (2012) Enhanced productivity of biomass and bioactive compounds through bioreactor cultures of Eleutherococcus koreanum Nakai adventitious roots affected by medium salt strength. Ind Crops Prod 36:460–465

    Article  CAS  Google Scholar 

  24. Lan ML, Piao XC, Yang CS, Paek KY (2001) Effect of MS medium strength and nitrogen concentration on bulblet formation and growth of Lilium in vitro. Kor J Plant Tiss Cult 28:341–346

    Google Scholar 

  25. Baque MA, Lee EJ, Paek KY (2010) Medium salt strength induced changes in growth, physiology and secondary metabolite content in adventitious roots of Morinda citrifolia: the role of antioxidant enzymes and phenylalanine ammonia lyase. Plant Cell Rep 29:685–694

    Article  Google Scholar 

  26. Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. Kor J Plant Tiss Cult 27:309–315

    Google Scholar 

  27. Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Proc Biochem 33:671–675

    Article  CAS  Google Scholar 

  28. Murthy HN, Praveen N (2011) Influence of macro elements and nitrogen source on adventitious root growth and withanolide-A production in Withania somnifera (L.) Dunal. Nat Prod Res 1:1–8

    Google Scholar 

  29. Yu KW, Gao WY, Hahn EJ, Paek KY (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in Ginseng (Panax ginseng C.A. Meyer). J Plant Biol 44:179–184

    Article  CAS  Google Scholar 

  30. Cui XH, Murthy HN, Wu CH, Paek KY (2010) Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites. In Vitro Cell Dev Biol 46:437–444

    Article  CAS  Google Scholar 

  31. Ahn JK, Lee WY, Park SY (2003) Effect of nitrogen source on the cell growth and production of secondary metabolites in bioreactor cultures of Eleutherococcus senticosus. Kor J Plant Biotechnol 30:301–305

    Article  Google Scholar 

  32. Morkunas I, Marczak L, Stachowiak J, Stobiecki M (2005) Sucrose-induced lupine defense against Fusarium oxysporum sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum. Plant Phyl Biochem 43:363–373

    Article  CAS  Google Scholar 

  33. Wind J, Smeekens S, Hanson J (2010) Sucrose: metabolite and signaling molecule. Phytochemistry 71:1610–1614

    Article  PubMed  CAS  Google Scholar 

  34. Shohael AM, Chakrabarty D, Ali MB, Yu KW, Hahn EJ, Lee HL, Paek KY (2006) Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress. Proc Biochem 41:512–518

    Article  CAS  Google Scholar 

  35. Zhang YH, Zhong JJ, Yu JT (1996) Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: manipulation of medium sucrose. J Biotechnol 51:49–56

    Article  CAS  Google Scholar 

  36. Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci 163:117–123

    Article  CAS  Google Scholar 

  37. Li SW, Xue L, Xu S, Feng H, An L (2009) Hydrogen peroxide acts as signal molecule in the adventitious roots formation of mungbean seedlings. Env Exp Bot 65:63–71

    Article  CAS  Google Scholar 

  38. Melillo MT, Leonettil P, Bongiovanni M, Astagnone SP, Bleve ZT (2006) Modulation of reactive oxygen species actives and H2O2 during compatible and incompatible tomato-root-knot nematode interactions. New Physiol 170:501–512

    Article  CAS  Google Scholar 

  39. Hung KT, Hsu YT, Kao CH (2006) Hydrogen peroxide is involved in methyl jasmonate-induced senescence of rice leaves. Physiol Plant 127:293–303

    Article  CAS  Google Scholar 

  40. Houot V, Etienne P, Petitot AS, Barbier S, Blein JP, Suty L (2001) Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner. J Exp Bot 52:1721–1730

    Article  PubMed  CAS  Google Scholar 

  41. Sakamoto A, Murata N (2002) The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant Cell Environ 25:163–171

    Article  PubMed  CAS  Google Scholar 

  42. Kim HK, Bang CS, Choi YM, Lee JS (2007) Antioxidant and antiproliferative activities of methanol extracts from leafy vegetables consumed in Korea. Food Sci Biotechnol 16:802–806

    CAS  Google Scholar 

  43. Cui XH, Murthy HM, Wu CH, Paek KY (2010) Sucrose induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell Tiss Organ Cult 103:7–14

    Article  CAS  Google Scholar 

  44. Baque MA, Elgirban A, Lee EJ, Paek KY (2012) Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.). Acta Phyl Plant 34:405–415

    Article  CAS  Google Scholar 

  45. Hu FX, Zhong JJ (2008) Jasmonic acid mediates gene transcription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jasmonate. Proc Biochem 43:113–118

    Article  CAS  Google Scholar 

  46. Murthy HM, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24:711–716

    Article  CAS  Google Scholar 

  47. Kang SM, Jung HY, Kang YM, Yun DJ, Bahk JD, Yang JK, Choi MS (2004) Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 166:745–751

    Article  CAS  Google Scholar 

  48. Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215

    Article  CAS  Google Scholar 

  49. Ali MB, Yu KY, Hahn EJ, Paek KY (2006) Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors. Plant Cell Rep 25:613–620

    Article  PubMed  CAS  Google Scholar 

  50. Dong X (1998) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316–323

    Article  PubMed  CAS  Google Scholar 

  51. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  PubMed  CAS  Google Scholar 

  52. Wang YD, Wu JC, Yuan YJ (2007) Salicylic acid-induced taxol production and isopentenyl pyrophosphate biosynthesis in suspension cultures of Taxus chinensis var. mairei. Cell Biol Int 31:1179–1183

    Article  PubMed  CAS  Google Scholar 

  53. Abbasi BH, Tian CL, Murch SJ, Saxena PK, Liu CZ (2007) Light-enhanced caffeic acid biosynthesis in hair root cultures of Echinacea purpurea. Plant Cell Rep 26:1367–1372

    Article  PubMed  CAS  Google Scholar 

  54. Sircar D, Gardoso HG, Mukherjee C, Mitra A, Arnholdt-Schmitt B (2012) Alternative oxidase (AOX) and phenolic metabolism in methyl jasmonate-treated hairy root cultures of Daucus carota L. J Plant Physiol 169:657–663

    Article  PubMed  CAS  Google Scholar 

  55. Ali MB, Yu KY, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Meyer and Panax quinquefolius L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Well Green Company Limited, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Eun-Jung Lee

  2. BIO-FD&C Company Limited, Smart Valley A-510, 30, Songdomirae-ro, Yeonsu-gu, Incehon, Republic of Korea

    Sang-Hyun Moh

  3. Department of Horticulture, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    So-Young Park

Authors
  1. Eun-Jung Lee
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  2. Sang-Hyun Moh
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  3. So-Young Park
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Corresponding author

Correspondence to Eun-Jung Lee .

Editor information

Editors and Affiliations

  1. Department of Horticultural Science, Chungbuk National University, Cheongju, Korea, Republic of (South Korea)

    Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, India

    Hosakatte Niranjana Murthy

  3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

    Jian-Jiang Zhong

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Lee, EJ., Moh, SH., Park, SY. (2014). Production of Biomass and Bioactive Compounds in Adventitious Root Cultures of Eleutherococcus koreanum Nakai. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_10

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