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Alleviation of heavy metal stress in Spilanthes calva L. (antimalarial herb) by exogenous application of glutathione

  • Abiotic Stress Responses
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Abstract

To evaluate the role of exogenous application of a phytochelating agent glutathione in increasing resistance against different heavy metals stress, nodal explants excised from 28-day-old in vitro seedlings of Spilanthes calva L. were cultured on Murashige and Skoog’s medium supplemented with 10 μM benzyl adenine and five different concentrations (1, 5, 50, 100, or 200 mg/l) of four heavy metals: As2O3, CuSO4, ZnSO4, or Pb(NO3)2. Data were recorded for percent survival, shoot number, and shoot length after 28 d of heavy metal treatment. All four heavy metals severely inhibited growth and morphogenesis. Pb proved most inhibitory whereas Zn was least effective. Pb was further selected to study the reversal effect of glutathione on morphogenesis. The addition of different concentrations (1, 5, 10, or 25 mg/l) of glutathione to media containing the Pb resulted in a significant improvement in almost all growth parameters. Inclusion of glutathione at 10 mg/l was optimum for maximum reversal of the negative effects of heavy metals on morphogenesis.

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References

  • Agrawal V.; Sharma K. Phytotoxic effects of Cu, Zn, Cd, and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica. Biol. Plant. 50: 307–310; 2006.

    Article  CAS  Google Scholar 

  • Alaoui-Sosse B.; Genet B. P.; Vinit-Dunad F.; Marie-Laure T.; Epron D.; Badot P. M. Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Sci 30: 1–6; 2004.

    Google Scholar 

  • Ammar W. B.; Mediounic C.; Tray B.; Ghorbel M.; Jemal F. Glutathione and phytochelatin contents in tomato plants exposed to cadmium. Biol. Plant. 52: 314–320; 2008.

    Article  Google Scholar 

  • Briat J.-F.; Lebrun M. Plant responses to metal toxicity. Comptes Rendus de l 'AcadeUmie des Sciences de Paris 322: 43–54; 1999.

    CAS  Google Scholar 

  • Bruns I.; Sutter K. Cadmium lets increase the glutathione pool in bryophytes. J. Plant Physiol. 158: 79–89; 2001.

    Article  CAS  Google Scholar 

  • Choi J. M.; Pak C. H.; Lee C. W. Micronutrient toxicity in French marigold. J. Plant Nutr. 19: 901–916; 1996.

    Article  CAS  Google Scholar 

  • Clemens S. Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88: 1707–1719; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Cobbett C.; Goldsbrough P. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu. Rev. Plant Biol. 53: 159–182; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Cosio C.; Martinoia E.; Keller C. Hyperaccumulation of Cadmium and Zinc in Thlaspi caerulescens and Arabidopsis halleri at the leaf cellular level. Plant Physiol. 134: 716–725; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Demiral T.; Türkan I. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot. 53: 247–257; 2005.

    Article  CAS  Google Scholar 

  • Dong J.; Wu F. B.; Zhang G. P. Influence of cadmium on antioxidant capacity and four microelement concentrations in tomato seedlings (Lycopersicon esculentum). Chemosphere 64: 1659–1666; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Ebbs S. D.; Kochian L. V. Toxicity of zinc and copper to Brassica species: implications for phytoremediation. J. Environ. Qual. 26: 776–781; 1997.

    Article  CAS  Google Scholar 

  • Fontes R. L. S.; Cox F. R. Zinc toxicity in soybean grown at high iron concentration in nutrient solution. J. Plant Nutr. 21: 1723–1730; 1998.

    Article  CAS  Google Scholar 

  • Freeman J. L.; Persans M. W.; Nieman K.; Albrecht C.; Peer W.; Pickering I. J.; Salt D. E. Increased glutathione biosynthesis plays a role in nickel tolerance in Thlaspi nickel hyperaccumulators. Plant Cell. 16: 2176–2191; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Fusconi A.; Repetto O.; Bona E.; Massa N.; Gallo C.; Dumas-Gaudot E.; Berta G. Effects of cadmium on meristem activity and nucleus ploidy in roots of Pisum sativum L. cv. Frisson seedlings. Environ. Exp. Bot. 58: 253–260; 2006.

    Article  CAS  Google Scholar 

  • Gallego S. M.; Benavides M. P.; Tomaro M. L. Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci 121: 151–159; 1996.

    Article  CAS  Google Scholar 

  • Hsu Y. T.; Kao C. H. Toxicity in leaves of rice exposed to cadmium is due to hydrogen peroxide accumulation. Plant Soil 298: 231–241; 2007.

    Article  CAS  Google Scholar 

  • Kastori R.; Petrovic M.; Petrovic N. Effects of excess lead, cadmium, copper and zinc on water relations in sunflower. J. Plant Nutr. 15: 2427–2439; 1992.

    Article  CAS  Google Scholar 

  • Kováčik J.; Bačkor M. Oxidative status of Matricaria chamomilla plants related to cadmium and copper uptake. Ecotoxicology 17: 471–479; 2008.

    Article  PubMed  Google Scholar 

  • Lewis S.; Donkin M. E.; Depledge M. H. Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors. Aquat. Toxicol. 51: 277–291; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Lyubenova L.; Schroder P. Uptake and effect of heavy metals on the plant detoxification cascade in the presence and absence of organic pollutants. Soil Biol. 19: 65–85; 2010.

    Article  CAS  Google Scholar 

  • Maksymiec W. Signaling responses in plants to heavy metal stress. Acta. Physiol. Plant 29: 177–187; 2007.

    Article  CAS  Google Scholar 

  • Mallick N.; Mohn F. H. Use of chlorophyll fluorescence in metal-stress research: a case study with the green micro alga Scenedesmus. Ecotoxicol Environ. Safety 55: 64–69; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Marin A. R.; Pezeshki S. R.; Masscheleyn P. H.; Choi H. S. Effect of dimethyl arsenic acid (DMAA) on growth, tissue arsenic, and photosynthesis of rice plants. J. Plant Nutr. 16: 865–880; 1993.

    Article  CAS  Google Scholar 

  • May M. J.; Vernoux T.; Leaver C.; Van Montagu M.; Inze D. Glutathione homeostasis in plants: implication for environmental sensing and plant development. J. Exp. Bot. 49: 649–667; 1998.

    Article  CAS  Google Scholar 

  • Mithöfer A.; Schulze B.; Boland W. Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Lett. 566: 1–5; 2004.

    Article  PubMed  Google Scholar 

  • Mishra S.; Srivastava S.; Tripathi R. D.; Kumar R.; Seth C. S.; Gupta D. K. Lead detoxification by coontail (Ceratophyllum demersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere 65: 1027–1039; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 431–487; 1962.

    Article  Google Scholar 

  • Nakatani N.; Nagashima M. Pungent alkamides from Spilanthes acmella L. var. oleracea Clarke. Biosc. Biotech. Biochem. 56: 759–762; 1992.

    Article  CAS  Google Scholar 

  • Pandey V.; Agrawal V.; Raghavendra K.; Dash A. P. Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Liston, Anopheles culicifacies, species C) and filaria vector (Culex quinquefasciatus Say). Parasitol. Res. 102: 171–174; 2007.

    Article  PubMed  Google Scholar 

  • Pandey V.; Chopra M.; Agrawal V. In vitro isolation and characterization of biolarvicidal compounds from micropropagated plants of Spilanthes acmella. Parasitol. Res. 108: 297–304; 2011.

    Article  PubMed  Google Scholar 

  • Perfus-Barbeoch L.; Leonhardt N.; Vavasseur A.; Forestier C. Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant J. 32: 539–548; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Qadir S.; Qureshi M. I.; Javed S.; Abdin M. Z. Genotypic variation in phytoremediation potential of Brassica juncea cultivars exposed to Cd stress. Plant Sci. 167: 1171–1181; 2004.

    Article  CAS  Google Scholar 

  • Ramsewak R. S.; Erickson A. J.; Nair M. G. Bioactive Nisobutylamides from the flower buds of Spilanthes acmella. Phytochemistry 51: 729–732; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Reddy A. M.; Kumar S. G.; Jyonthsnakumari G.; Thimmanaik S.; Sudhakar C. Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.). Chemosphere 60: 97–104; 2005.

    Article  PubMed  CAS  Google Scholar 

  • Salt D. E.; Rauser W. E. Mg-ATP-dependent transport of phytochelatins across the tonoplast of oat roots. Plant Physiol. 107: 1293–1301; 1995.

    PubMed  CAS  Google Scholar 

  • Sandalio L. M.; Dalurzo H. C.; Gomez M.; Romero-Puertas M. C.; Del-Rio L. A. Cadmium induces changes in the growth and oxidative metabolism of pea plants. J. Exp. Bot. 52: 2115–2126; 2001.

    PubMed  CAS  Google Scholar 

  • Sckerl M. M.; Frans R. E. Translocation and metabolism of MAA-14C in johnsongrass and cotton. Weed Sci. 17: 421–427; 1969.

    CAS  Google Scholar 

  • Sharma P.; Dubey R. S. Lead toxicity in plants. Braz. J. Pl. Physiol. 17: 35–52; 2005.

    Article  CAS  Google Scholar 

  • Sinha P.; Dubey B. K.; Srivastava P.; Chatterjee C. Alteration in uptake and translocation of essential nutrients in cabbage by lead excess. Chemosphere 65: 651–656; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Singh R. P.; Tripathi R. D.; Dabas S.; Rizvi S.; Ali M. H.; Sinha M. B.; Gupta S. K.; Mishra D. K.; Rai U. N. Effect of lead on growth and nitrate assimilation of Vigna radiata (L.) seedlings in a salt affected environment. Chemosphere 52: 1245–1250; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Terwelle H. F.; Slater E. C. Uncoupling of respiratory chain phosphorylation by arsenate. Biochem. Biophys. Acta. 143: 1–17; 1967.

    Article  CAS  Google Scholar 

  • Vara Prasad M. N. Metal hyper accumulation in plants-biodiversity prospecting for phytoremediation technology. Eleat. Jr. Biotech. 6: 517–528; 2003.

    Google Scholar 

  • Verbruggen N.; Hermans C.; Schat H. Molecular mechanisms of metal hyperaccumulation in plants. New Phytol. 181: 759–772; 2009.

    Article  PubMed  CAS  Google Scholar 

  • Yadav S. K. Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S. Afr. J. Bot. 76: 167–179; 2009.

    Article  Google Scholar 

  • Zeng L. S.; Liao M.; Chen C. L.; Huang C. Y. Effect of lead contamination on soil enzymatic activities, microbial biomass and rice physiology induces in soil-lead-rice (Oryza sativa L.) system. Ecol. Environ. Bot. 67: 67–74; 2007.

    CAS  Google Scholar 

  • Zenk M. H. Heavy metal detoxification in higher plants a review. Gene 179: 21–30; 1996.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to the University Grant Commission, New Delhi, India and Delhi University, India for their financial assistance. VS is indebted to Council of Scientific and Industrial Research, New Delhi, India for JRF-SRF.

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Vinay Shankar, Veena Thekkeettil, Gaurav Sharma & Veena Agrawal

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  1. Vinay Shankar
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  2. Veena Thekkeettil
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  3. Gaurav Sharma
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  4. Veena Agrawal
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Correspondence to Veena Agrawal.

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Editor: J. Finer

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Shankar, V., Thekkeettil, V., Sharma, G. et al. Alleviation of heavy metal stress in Spilanthes calva L. (antimalarial herb) by exogenous application of glutathione. In Vitro Cell.Dev.Biol.-Plant 48, 113–119 (2012). https://doi.org/10.1007/s11627-011-9409-9

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