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Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity

  • Plant Tissue Culture
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Abstract

The photochemical reflectance index (PRI) of micropropagated potato leaves was estimated nondestructively from outside the culture vessel using a PRI imaging system developed by the present group. The PRI was determined under low light intensity conditions after dark treatment and compared with the chlorophyll fluorescence parameter Fv/Fm, which denotes photosystem II maximum quantum yield. Short-term high-light treatment decreased Fv/Fm of the plantlets. Culture conditions such as temperature and sucrose concentration also affected Fv/Fm. A linear relationship between the PRI and Fv/Fm was observed in both cases of high-light treatment and different culture conditions, suggesting the potential of the PRI to be used as a substitute for Fv/Fm. PRI estimated from reflection images under low light intensity conditions may be used for rapid and noninvasive evaluation of photosynthetic properties of micropropagated plantlets in a similar manner to Fv/Fm.

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References

  • Aitken-Christie J.; Davies H. E.; Kubota C.; Fujiwara K. Effect of nutrient media composition on sugar-free growth and chlorophyll fluorescence of Pinus radiata shoots in vitro. Acta. Hortic. 319: 125–130; 1992.

    Google Scholar 

  • Arigita L.; González A.; Tamés R. S. Influence of CO2 and sucrose on photosynthesis and transpiration of Actinidia deliciosa explants cultured in vitro. Physiol. Plant. 115: 166–173; 2002.

    Article  CAS  PubMed  Google Scholar 

  • Filella I.; Peñuelas J.; Llorens L.; Estiarte M. Reflectance assessment of seasonal and annual changes in biomass and CO2 uptake of a Mediterranean Shrubland submitted to experimental warming and drought. Remote Sens. Environ. 90: 308–318; 2004.

    Article  Google Scholar 

  • Gamon J. A.; Field C. B.; Bilger W.; Björkman O.; Fredeen A. L.; Peñuelas J. Remote sensing of the xanthophyll cycle and chlorophyll fluorescence in sunflower leaves and canopies. Oecologia 85: 1–7; 1990.

    Article  Google Scholar 

  • Gamon J. A.; Serrano L.; Surfus J. S. The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels. Oecologia 112: 492–501; 1997.

    Article  Google Scholar 

  • Hall F. G.; Hilker T.; Coops N. C.; Lyapustin A.; Huemmrich K. F.; Middleton E.; Margolis H.; Drolet G.; Black T. A. Multi-angle remote sensing of forest light use efficiency by observing PRI variation with canopy shadow fraction. Remote Sens. Environ. 112: 3201–3211; 2008.

    Article  Google Scholar 

  • Hofman P.; Haisel D.; Komenda J.; Vágner M.; Tichá I.; Schäfer C.; Čapková V. Impact of in vitro cultivation conditions on stress responses and on changes in thylakoid membrane proteins and pigments of tobacco during ex vitro acclimation. Biol. Plant. 45: 189–195; 2002.

    Article  CAS  Google Scholar 

  • Ibaraki Y. Evaluation of photosynthetic capacity in micropropagated plants by image analysis. In: Dutta Gupta S.; Ibaraki Y. (eds) Plant tissue culture engineering. Springer, The Netherlands, pp 15–29; 2006.

    Google Scholar 

  • Ibaraki Y.; Iida Y.; Kurata K. Effects of air currents on gas exchange of culture vessels. Acta. Hortic. 319: 221–224; 1992.

    Google Scholar 

  • Ibaraki Y.; Kurata K. Application of image analysis to plant cell suspension cultures. Comput. Electron. Agric. 30: 193–203; 2001.

    Article  Google Scholar 

  • Ibaraki Y.; Matsumura K. Non-destructive evaluation of the photosynthetic capacity of PSII in micropropagated plants. J. Agric. Meteorol. 60: 1073–1076; 2005.

    Google Scholar 

  • Ibaraki Y.; Matsumura K.; Dutta Gupta S. Low-cost photochemical reflectance index measurements of micropropagated plantlets using image analysis. Comput. Electron. Agric. 71: 170–175; 2010.

    Article  Google Scholar 

  • Ibaraki Y.; Nozaki Y. Estimation of light intensity distribution in a culture vessel. Plant Cell Tissue Organ Cult. 80: 111–113; 2005.

    Article  Google Scholar 

  • Jo E.-A.; Tewari R. K.; Hahn E.-J.; Paek K.-Y. In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets. Plant Cell Tissue Organ Cult. 96: 307–315; 2009.

    Article  CAS  Google Scholar 

  • Kato M. C.; Hikosaka K.; Hirose T. Leaf discs floated on water are different from intact leaves in photosynthesis and photoinhibition. Photosynth. Res. 72: 65–70; 2002.

    Article  CAS  PubMed  Google Scholar 

  • Király I.; Balla I.; Jakab J.; Tamás L.; Sárvári E. Responses of the photosynthetic system and peroxidase activity to the rooting conditions of osk micropropagation. Plant Cell Tissue Organ Cult. 66: 155–158; 2001.

    Article  Google Scholar 

  • Kozai T.; Smith M. A. L. Environmental control in plant tissue culture. General introduction and overview. In: Aitken-Christie J.; Kozai T.; Smith M. A. L. (eds) Automation and environmental control in plant tissue cultures. Kluwer Academic Publishers, Dordrecht, pp 301–318; 1995.

    Google Scholar 

  • Kubota C. Concepts and background of photoautotrophic micropropagation. In: Morohoshi N.; Komamine A. (eds) Molecular breeding of woody plants. Elsevier, Amsterdam, pp 325–334; 2001.

    Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.

    Article  CAS  Google Scholar 

  • Nakaji T.; Oguma H.; Fujinuma Y. Seasonal changes in the relationship between photochemical reflectance index and photosynthetic light use efficiency of Japanese larch needles. Int. J. Remote Sens. 27: 493–509; 2006.

    Article  Google Scholar 

  • Naumann J. C.; Young D. R.; Anderson J. E. Liking leaf chlorophyll fluorescence properties to physiological responses for detection of salt and drought stress in coastal plant species. Physiol. Plant. 131: 422–433; 2007.

    Article  CAS  PubMed  Google Scholar 

  • Naumann J. C.; Young D. R.; Anderson J. E. Leaf chlorophyll fluorescence, reflectance, and physiological response to fresh water and salt water flooding in the ever green shrub, Myricacerifera. Environ. Exp. Bot. 63: 402–409; 2008.

    Article  CAS  Google Scholar 

  • Serret M. D.; Trillas M. I.; Araus J. L. The effect of in vitro culture conditions on the pattern of photoinhibition during acclimation of gardenia plantlets to ex vitro conditions. Photosynthetica 39: 67–73; 2001.

    Article  CAS  Google Scholar 

  • Smith M. A. L. Image analysis for plant tissue culture and micropropagtion. In: Aitken Christie J.; Kozai T.; Smith M. A. L. (eds) Automation and environmental control in plant tissue cultures. Kluwer Academic Publishers, Dordrecht, pp 145–163; 1995.

    Google Scholar 

  • Stylinski C. D.; Gamon J. A.; Oechel W. C. Seasonal patterns of reflectance indices, carotenoid pigments and photosynthesis of evergreen chaparral species. Oecologia 131: 366–374; 2002.

    Article  Google Scholar 

  • Suárez L.; Zarco-Tejada P. J.; Berni J. A. J.; González-Dugo V.; Fereres E. Modelling PRI for water stress detection using radiative transfer models. Remote Sens. Environ. 113: 730–744; 2009.

    Article  Google Scholar 

  • Váňová M.; Kummerová M.; Klemš M.; Zezulkaet S. Fluoranthene influences endogenous abscisic acid level and primary photosynthetic processes in pea (Pisum sativum L.) plants in vitro. Plant Growth Regul. 57: 39–47; 2009.

    Article  Google Scholar 

  • Weng J.-H.; Lai K.-M.; Liao T.-S.; Hwang M.-Y.; Chen Y.-N. Relationships of photosynthetic capacity to PSII efficiency and to photochemical reflectance index of Pinus taiwanensis through different seasons at high and low elevations of sub-tropical Taiwan. Trees 23: 347–356; 2009.

    Article  CAS  Google Scholar 

  • Yamamoto H. Y. Biochemistry of violaxanthin cycle in higher plant. Pure Appl. Chem. 51: 639–648; 1979.

    Article  CAS  Google Scholar 

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Acknowledgments

This study was conducted with the financial support from DST, New Delhi, India and Japan Society for the Promotion of Science, Tokyo, Japan.

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Authors and Affiliations

  1. Faculty of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515, Japan

    Yasuomi Ibaraki

  2. Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India

    S. Dutta Gupta

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  1. Yasuomi Ibaraki
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  2. S. Dutta Gupta
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Correspondence to Yasuomi Ibaraki.

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Editor: Y. Takahata

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Ibaraki, Y., Dutta Gupta, S. Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity. In Vitro Cell.Dev.Biol.-Plant 46, 530–536 (2010). https://doi.org/10.1007/s11627-010-9296-5

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  • DOI: https://doi.org/10.1007/s11627-010-9296-5

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