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In vitro propagation of brown oak (Quercus semecarpifolia Sm.) from seedling explants

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Abstract

An efficient and reproducible method for the regeneration of multiple shoots of brown oak (Quercus semecarpifolia Sm.) has been developed in which a part of the petiolar tube containing a primary shoot is used as the explant. Explants derived from in vitro grown seedlings were cultured either on Murashige and Skoog or Woody Plant medium (WPM) containing different concentrations of benzyladenine (BAP) throughout the range of 1–20 μM. WPM supplemented with 20 μM BAP was found to be best for adventitious shoot induction and for the multiplication of individual shoots. In-vitro-produced shoots were rooted using a two-step method. Firstly, shoots were cultured on WPM containing indolebutyric acid (IBA) at either 50 or 100 μM for 24 or 48 h. Secondly, the shoots were transferred to plant-growth-regulator-free half-strength WPM. The second step not only considerably improved the rooting percentage but also minimized the formation of basal callus. The most effective first-step treatment was found to be 100 μM IBA for 24 h, which initiated rooting at a frequency of 100%. Well-rooted plants were transferred to plastic cups containing nonsterile, sieved soil and farmyard manure, hardened under greenhouse conditions, and then successfully established in pots. This procedure is suitable for use in large-scale production of plants and may have potential application in additional oak species.

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Acknowledgements

Financial support of this study was received from the Department of Biotechnology, Govt. of India. The assistance of Drs. A. Kumar, N. Bag, P. Vyas, and M.S. Bisht is gratefully acknowledged.

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Authors and Affiliations

  1. G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, U. A., India

    Sushma Tamta, Lok Man S. Palni, Vijay K. Purohit & Shyamal K. Nandi

  2. Botany Department, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand, 263 002, India

    Sushma Tamta

  3. State Biotechnology Programme, Government of Uttaranchal, Biotec Bhavan, P.O. Haldi, Pantnagar, 263 146, Uttarakhand, India

    Lok Man S. Palni

  4. Kumaun University, Nainital, Uttarakhand, 263 002, India

    Sushma Tamta

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  1. Sushma Tamta
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  2. Lok Man S. Palni
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  3. Vijay K. Purohit
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  4. Shyamal K. Nandi
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Correspondence to Sushma Tamta.

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Editor: Florent Engelmann

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Tamta, S., Palni, L.M.S., Purohit, V.K. et al. In vitro propagation of brown oak (Quercus semecarpifolia Sm.) from seedling explants. In Vitro Cell.Dev.Biol.-Plant 44, 136–141 (2008). https://doi.org/10.1007/s11627-008-9138-x

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  • DOI: https://doi.org/10.1007/s11627-008-9138-x

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