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miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5

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Abstract

Development of skeletal muscle is a complicated biological process regulated by various regulation factors and signal pathways. MicroRNAs (miRNAs) are novel gene regulators that control muscle cell development. microRNA-143 (miR-143) is highly expressed in skeletal muscle, and we found that miR-143 level is significantly increased during bovine skeletal muscle satellite cells (MSCs) differentiation process through microarray analysis and qRT-PCR detection. However, the function of miR-143 in bovine muscle development remained unclear. In our work, the functions of miR-143 in bovine MSCs myogenic differentiation were investigated. We discovered that IGFBP5 is directly regulated by miR-143 using a dual-luciferase reporter assay. Overexpression of miR-143 led to decreased level of IGFBP5 protein and restrained cell proliferation and differentiation, while downregulation of miR-143 resulted in increased levels of IGFBP5 protein and restrained cell proliferation but improved differentiation. IGFBP5, an important component of IGF signaling pathway, contributes greatly to bovine muscle cell development. A mechanism that miR-143 can regulate the proliferation and differentiation of bovine MSCs through changing expression of IGFBP5 was elucidated by our study.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31201021), the Training Programs of Innovation and Entrepreneurship for Undergraduates of Tianjin (201510061006), and the 131 Innovative Talents Cultivation Project in Tianjin (J01005021104).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

    Wei Ran Zhang, Hui Na Zhang, Yi Min Wang, Yang Dai, Xin Feng Liu, Xin Li, Xiang Bin Ding & Hong Guo

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  1. Wei Ran Zhang
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  2. Hui Na Zhang
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  3. Yi Min Wang
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  4. Yang Dai
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  5. Xin Feng Liu
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  6. Xin Li
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  7. Xiang Bin Ding
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  8. Hong Guo
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Correspondence to Xiang Bin Ding or Hong Guo.

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Editor: Tetsuji Okamoto

Wei Ran Zhang and Hui Na Zhang contributed equally to this work.

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Zhang, W.R., Zhang, H.N., Wang, Y.M. et al. miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5. In Vitro Cell.Dev.Biol.-Animal 53, 265–271 (2017). https://doi.org/10.1007/s11626-016-0109-y

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  • DOI: https://doi.org/10.1007/s11626-016-0109-y

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