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The role of microRNA-1 and microRNA-206 in the proliferation and differentiation of bovine skeletal muscle satellite cells

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Abstract

MicroRNAs (miRNAs) have been found to play essential roles in muscle cell proliferation and differentiation. MicroRNA-1 (miR-1) and microRNA-206 (miR-206), which are similar and have the same seed sequence, have specific roles in modulating skeletal muscle proliferation and differentiation in vitro and in vivo. However, there is no information about their function during bovine skeletal muscle satellite cell development. In this study, the profiles of miR-1 and miR-206 and their biological functions in bovine skeletal muscle cell development was investigated. The target genes were predicted, and we used a dual-luciferase reporter assay to demonstrate that miR-1 and miR-206 directly targeted the 3′ untranslated region (3′UTR) of paired-box transcription factor Pax7 and histone deacetylase 4 (HDAC4). We showed that miR-1 and miR-206 facilitate bovine skeletal muscle satellite cell myogenic differentiation by restricting the expression of their target gene and that inhibition of miR-1 and miR-206 increased the Pax7 and HDAC4 protein levels and substantially enhanced satellite cell proliferation. Therefore, our results revealed the mechanism in which miR-1 and miR-206 positively regulate bovine skeletal muscle satellite cell myogenic differentiation via Pax7 and HDAC4 downregulation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31201021), Natural Science Foundation of Tianjin (13JCQNJC14600), the Excellent Young Teachers Program of Tianjin and the Veterinary Biotechnology Scientific Research Innovation Team of Tianjin (TD12-5019).

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    Authors and Affiliations

    1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

      Yang Dai, Yi Min Wang, Wei Ran Zhang, Xin Feng Liu, Xin Li, Xiang Bin Ding & Hong Guo

    2. College of Veterinary Medicine and Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China

      Yi Min Wang

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    1. Yang Dai
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    2. Yi Min Wang
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    3. Wei Ran Zhang
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    4. Xin Feng Liu
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    5. Xin Li
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    6. Xiang Bin Ding
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    7. Hong Guo
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    Correspondence to Xiang Bin Ding or Hong Guo.

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    Editor: Tetsuji Okamoto

    Yang Dai and Yi Min Wang contributed equally to this work.

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    Dai, Y., Wang, Y.M., Zhang, W.R. et al. The role of microRNA-1 and microRNA-206 in the proliferation and differentiation of bovine skeletal muscle satellite cells. In Vitro Cell.Dev.Biol.-Animal 52, 27–34 (2016). https://doi.org/10.1007/s11626-015-9953-4

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    • DOI: https://doi.org/10.1007/s11626-015-9953-4

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