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Establishment and characterization of a cell line developed from the neonate larvae of Papilio demoleus Linnaeus (Lepidoptera: Papilionidae)

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Abstract

A new cell line named RIRI-PaDe, developed from the neonate larvae of Papilio demoleus Linnaeus, was established in modified Grace’s medium supplemented with 20% fetal bovine serum. The cell line was incubated at 28°C and consisted of attached round and short spindle-like cells. The population doubling time was 55 h. The chromosome numbers varied widely from 24 to 136 with a mode of 59 at the 71st passage. Comparison of mitochondrial cytochrome c oxidase subunit I gene of the cell line and neonate larvae confirmed that the cell line was of P. demoleus origin. This cell line was susceptible to the Autographa californica multicapsid nucleopolyhedrovirus and Apocheima cinerarius nucleopolyhedrovirus.

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Acknowledgments

This work was supported by grants from the National Ministry of Forestry of China (no. 2013-4-53) and Chinese Academy of Forestry (no. riricaf2012003M). We thank Dr. Ping Tang of the Jiangsu University for providing AcMNPV and Prof. Qilian Qin of the Institute of Zoology, Chinese Academy of Sciences, for providing AciNPV, HycuNPV, LdNPV, and AnpeNPV. We are grateful to Ms. Shanda Wheeler, in the Department of Entomology, Kansas State University, for reading the manuscript and giving suggestions on English grammar and writing.

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Authors and Affiliations

  1. Research Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650224, Yunnan, China

    Wei-Feng Ding, Ying Feng, Xin Zhang, Xian Li & Cheng-Ye Wang

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  1. Wei-Feng Ding
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  2. Ying Feng
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  3. Xin Zhang
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  4. Xian Li
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  5. Cheng-Ye Wang
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Correspondence to Ying Feng.

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Ding, WF., Feng, Y., Zhang, X. et al. Establishment and characterization of a cell line developed from the neonate larvae of Papilio demoleus Linnaeus (Lepidoptera: Papilionidae). In Vitro Cell.Dev.Biol.-Animal 49, 108–113 (2013). https://doi.org/10.1007/s11626-012-9575-z

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  • DOI: https://doi.org/10.1007/s11626-012-9575-z

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