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Attachment Strength of Listeria monocytogenes and its Internalin-Negative Mutants

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Abstract

A single cell of Listeria monocytogenes attached on food contact surfaces can be a potential source of cross-contamination in a food-processing plant. To see whether internalin A (InlA) and B (InlB), major surface proteins on L. monocytogenes, play a significant role in the attachment process, wild-type L. monocytogenes EGD (LM_EGD) and its isogenic internalin-negative mutants (LM_EGDΔinlA, LM_EGDΔinlB, and LM_EGDΔinlAB) were used to determine attachment strength on inert glass surface. Western blot analysis using InlA and InlB antibodies confirmed the absence of InlA in LM_EGDΔinlA, InlB in LM_EGDΔinlB, and both InlA and InlB in LM_EGDΔinlAB. Regardless of initial attachment numbers, LM_EGD which expressed both InlA and InlB proteins exhibited the strongest attachment strength while the double mutant (LM_EGDΔinlAB) exhibited the weakest. The two single mutants (LM_EGDΔinlA and LM_EGDΔinlB) that expressed only one type of the internalins were shown to have intermediate attachment strength. These results suggest that both InlA and InlB expression play a significant role in the attachment strength of L. monocytogenes on glass surface.

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Acknowledgments

Approved for publication as Journal Article No.11053 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University. This work was supported in part by the Mississippi Agricultural and Forestry Experiment Station Project Number MIS-371272 and by USDA-ARS Grant No. 58-0790-5-137. We gratefully acknowledge Dr. Pascale Cossart (Départment de Biologie Cellulaire et Infection, INSTITUT PASTEUR, France) for kindly providing wild-type L. monocytogenes EGD and its mutant strains and Dr. Arun K. Bhunia for kindly providing anti-Listeria InlB antibody.

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Authors and Affiliations

  1. Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Box 9805, Starkville, MS, USA

    B. Y. Chen, T. J. Kim & J. L. Silva

  2. Department of Biochemistry and Molecular Biology, Mississippi State University, Starkville, MS, 39762, USA

    Y. S. Jung

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  1. B. Y. Chen
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  2. T. J. Kim
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  3. Y. S. Jung
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  4. J. L. Silva
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Correspondence to T. J. Kim.

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Chen, B.Y., Kim, T.J., Jung, Y.S. et al. Attachment Strength of Listeria monocytogenes and its Internalin-Negative Mutants. Food Biophysics 3, 329–332 (2008). https://doi.org/10.1007/s11483-008-9090-7

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  • DOI: https://doi.org/10.1007/s11483-008-9090-7

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