Abstract
Bacterial artificial chromosomes (BACs) or yeast artificial chromosomes (YACs) containing large inserts as probes for fluorescence in situ hybridization (FISH) have been used in the physical mapping of specific DNA sequences, especially for single- or low-copy sequences. Our earlier study identified Stpk-V, a powdery mildew resistance-related gene located on the 6VS chromosome arm of the wild grass Haynaldia villosa (tribe Triticeae), and obtained several Triticum aestivum-H. villosa alien chromosome lines carrying the Stpk-V gene. However, the precise physical location of the Stpk-V gene on chromosome 6VS is not known. In this study, we used TAC-FISH with TAC15 as the probe coupled with sequential genomic in situ hybridization (GISH) to determine the physical location of the Stpk-V gene in different T. aestivum-H. villosa 6V alien chromosome lines, including addition, substitution and translocation lines. The result indicated that the fraction length of the Stpk-V locus is 0.575±0.035 on the 6V chromosome short arm and this was confirmed by FISH using TAC15 as the probe for tracing the Stpk-V gene in other genetic stocks. The cytological mapping strategies used in this study will be of benefit for tracing the alien gene location in the course of introducing desirable traits from wild species.
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Jiang J, Gill B S. Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome, 2006, 49:1057–1068
Raina S N, Rani V. GISH technology in plant genome research. Methods Cell Sci, 2001, 23:83–104
Zhang P, Li W, Fellers J, et al. BAC-FISH in wheat identifies chromosome landmarks consisting of different types of transposable elements. Chromosoma, 2004, 112:288–299
Friebe B, Jiang J, Raupp W J, et al. Characterization of wheat-alien translocations conferring resistance to diseases and pests: Current status. Euphytica, 1996, 91:59–87
Jiang J, Gill B S, Wang G, et al. Metaphase and interphase fluorescence in situ hybridization mapping the rice genome with bacterial artificial chromosomes. Proc Natl Acad Sci USA, 1995, 92: 4487–4491
Ohmido N, Akiyama Y, Fukui K. Physical mapping of unique nucleotide sequences on identified rice chromosomes. Plant Mol Biol, 1998, 18:1043–1052
Rajyashri K R, Nair S, Ohmido N, et al. Isolation and FISH mapping of Yeast Artificial Chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice. Theor Appl Genet, 1998, 97:507–514
Qin R, Wei W, Jin W, et al. Physical location of rice Gm-6, Pi-5(t) genes in O. officinalis with BAC-FISH. Chin Sci Bull, 2001, 46:659–661
Xiong Z, Tan G, You A, et al. Comparative physical mapping of rice BAC clones linked to resistance gene Glh, Bph-3 and xa-5 in Oryza sativa L. and O. granulata Nees et Arn. Ex Watt. Chin Sci Bull, 2004, 49:591–596
Gómez M I, Islam-Faridi M N, Woo S, et al. FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor. Genome, 1997, 40:475–478
Suzuki G, Tanaka S, Yamamoto M, et al. Visualization of the S-locus region in Ipomoea trifida: Toward positional cloning of self-incompatibility genes. Chromosome Res, 2004, 12:475–481
Desel C, Jung C, Cai D, et al. High-resolution mapping of YACs and the single-copy gene Hs1 pro-1 on Beta vulgaris chromosomes by multi-color fluorescence in situ hybridization. Plant Mol Biol, 2001, 45:113–122
Chen P D, Qi L L, Zhou B, et al. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AV translocation lines specifying resistance to powdery mildew. Theor Appl Genet, 1995, 91:1125–1128
Qi L L, Wang S L, Chen P D, et al. Identification and physical mapping of three Haynaldia villosa chromosome-6V deletion lines. Theor Appl Genet, 1998, 97:1042–1046
Cao A Z, Wang X E, Chen Y P, et al. A sequene-specific PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa. Plant Breed, 2006, 125:201–205
Fan Y D, Liu Y G, Wu H, et al. Construction of a transformation-competent artificial chromosome (TAC) library of a wheat-Haynaldia villosa translocation line (in Chinese). Chin J Biotech, 2000, 16:433–436
Sun Y L, Cao A Z, Yang X M, et al. Screening Hv-S/TPK from TAC library of a Triticum aestivum-Haynaldia villosa translocation line (in Chinese). Chin J Biotech, 2008, 24:1327–1332
Cao A, Xing L, Wang X, et al. Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA, 2011, 108:7727–7732
Chen P D, Liu D J. Identification of Haynaldia villosa chromosomes in alien wheat alien addition lines. In: Li Z S, Swaminathan M S, eds. Proc 1st Int Symp on Chromosome Engineering in Plants, Xi’an, China, 1986. 31–33
Pei G Z, Chen P D, Liu D J. A cytogenetic analysis of some powdery mildew resistant strains of the hybrid progeny between wheat and Haynaldia villosa (in Chinese). J Nanjing Agric Univ, 1986, 1:1–9
Liu D J, Chert P D, Pei G Z, et al. Transfer of Haynaldia villosa chromosomes into Triticum aestivum. In: Miller T E, Koebner R M D, eds. Proc 7th Int Wheat Genet Symp, Cambridge, UK, 1988. 355–361
Chen P D, Zhang S Z, Wang X E, et al. New wheat variety Nannong 9918 with high yield and powdery mildew resistance (in Chinese). J Nanjing Agric Univ, 2002, 25:105–106
Chen S, Chen P, Wang X. Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line. Sci China Ser C Life Sci, 2008, 51:346–352
Qi L L, Wang S L, Chen P D, et al. Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat. Theor Appl Genet, 1997, 95:1084–1091
Zhang D, Yang Q, Bao W, et al. Molecular cytogenetic characterization of the Antirrhinum majus genome. Genetics, 2005, 169:325–335
Dong F, McGrath J M, Helgeson J P, et al. The genetic identity of alien chromosomes in potato breeding lines revealed by sequential GISH and FISH analyses using chromosome-specific cytogenetic DNA markers. Genome, 2001, 44:729–734
Lengerova M, Kejnovsky E, Hobza R, et al. Multicolor FISH mapping of the dioecious model plant, Silene latifolia. Theor Appl Genet, 2004, 108:1193–1199
Smith D B, Flavell R B. Characterization of the wheat genome by renaturation kinetics. Chromosoma, 1975, 50:223–242
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Yang, X., Cao, A., Sun, Y. et al. Tracing the location of powdery mildew resistance-related gene Stpk-V by FISH with a TAC clone in Triticum aestivum-Haynaldia villosa alien chromosome lines. Chin. Sci. Bull. 58, 4084–4091 (2013). https://doi.org/10.1007/s11434-013-5851-x
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DOI: https://doi.org/10.1007/s11434-013-5851-x