Abstract
A fluorescencein situ hybridization (FISH) procedure was adopted to physically map two rice BAC clones 24E21 and 4F22 linked toGm-6 andPi-5(t) inO. officinalis. FISH results showed that the two BAC clones were located at 4L. The percentage distance from the centromere to the hybridization sites was 72±2.62 for 24E21 and 54±5.43 for 4F22, the detection rates were 52.70% and 61.2%. The results obtained from the BAC and plasmid clones, RG214 and RZ565 of cultivated rice andO. officinalis were the same. This suggested that the markers, RG214 and RZ565 of cultivated rice andO. officinalis were in the same BAC clones. The homologous sequences ofGm-6 andPi-5(t) inO. officinalis were positions that signals existed on the 4L. Many signals were observed when no Cot-1 DNA blocked. This also showed that repetitive sequences were some homolgous between cultivated rice andO. officinalis. The identification of chromosome 4 ofO. officinalis is based on Jena et al. (1994). In our study, we discussed the possibility of physical map inO. officinalis with rice BAC clones.
Similar content being viewed by others
References
Zhang, S. Z., Lu, B. R., Hong, D. Y., The application ofin situ hybridization inOryza, Journal of Plant Classify (in Chinese), 1998, 1: 1.
Yang, D. C., Parco, A., Nandi, S. et al., Construction of a bacterial artificial chromosome (BAC) library and identification of overlapping BAC clones with chromosome 4-specific RFLP markers in rice, Theor. Appl. Genet., 1997, 95: 1147.
National Wild Rice Resource Investigation Cooperation Group, Chinese wild rice resource investigation, Chinese Agricultural Science, 1984, 6: 1.
Jena, K. K., Khush, G. S., Kocnert, G. et al., Comparative RFLP mapping of a wild rice,Oryza officinlis, and cultivated rice,O. sativa, Genome, 1994, 37(3): 383.
Yan, H. M., Song, Y. C., Li, L. J. et al., Physical location of the rice Pi-5(t),Glh andRTSV genes by ISH of BAC clones, J. Wuhan Univ. (Natural Science Edition), 1998, 3(2): 226.
Ren, N., Song, Y. C., Bi, X. Z., Ding, Y. et al., The physical location of genescdc2 andprh1 in maize (Zea mays L.), Hereditas, 1997, 126: 211.
Sambrook, J., Fritsch, E. F., Manitiatis, T., Molecular Cloning:A Laboratory Mannual, New York: Cold Spring Harbor Laboratory Press, 1989, 25.
Song, Y. C., Gustafson, J. P., The physical location of fourteen RFLP markers in rice (Oryza sativa), Theor. Appl. Genet., 1995, 90: 113.
Jiang, J., Gill, B. S., Wang, G. L. et al., Metaphase and interphase fluorescencein situ hybridization mapping of the rice genome with bacterial artificial chromosomes, Proc. Natl. Acad. Sci. USA, 1995, 92: 4487.
Causse, M. A., Theresa, M. F., Yang, G. C. et al., Saturated molecular map of rice genome based on interspecific backcross population, Genetics, 1994, 138: 1251.
Jiang, J., Gill, B. S., Nonisotopicin situ hybridization and plant genome mapping: the first ten years, Genome, 1994, 37(5): 717.
Shizuya, H., Brieen, B., Kim, U. J. et al., Cloning and stable maintenance of 300 kilobase-pair fragments of human DNA inEscherichia coli using an F-factor-based vector, Proc. Natl. Acad. Sci. USA, 1992, 89: 8794.
Qin, R., Wei, W. H., Song, Y. C., The application of BAC-FISH in plant genome research, Prog. Biophys (in Chinese), 2000, 27(1): 20.
Hanson, R. E., Zwick, M. S., Choi, S. et al., Fluorescentin situ hybridization of a bacterial artificial chromosomes, Genome, 1995, 38(4): 646.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Qin, R., Wei, W., Jin, W. et al. Physical location of riceGm-6, Pi-5(t) genes inO. officinalis with BAC-FISH. Chin. Sci. Bull. 46, 659–661 (2001). https://doi.org/10.1007/BF03182829
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03182829