Abstract
The fragmentation pathways of two isomers of brassinosteroids, 28-homobrassinolide (28-h-BL) and 28-epihomobrassinolide (28-eh-BL), have been investigated by tandem mass spectrometry (MSn, n = 1, 2, 3, 4) with the electrospray ionization (ESI) source. The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated, and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers.
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References
Mitchell J W, Mandava N, Worley J F, et al. Brassins: A new family of plant hormones from rape pollen. Nature, 1970, 225:1065–1066
Grove M D, Spencer G F, Rohwedder W K, et al. Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature, 1979, 281:216–217
Bajguza A, Tretynb A. The chemical characteristic and distribution of brassinosteroids in plants. Phytochemistry, 2003, 62:1027–1046
Schmidt J, Spengler B, Takao Y. Secasterone, the first naturally occurring 2,3-epoxybrassnosteroids from Secale cereale. Phytochemistry, 1995, 38:1095–1097
Gamoh K, Hiroyuki A, Katsuya S, et al. Liquid Chromatography/Mass Spectrometry with atmospheric pressure chemical ionization of free brassinosteroids. Rapid Commun Mass Spectrom, 1996, 10:903–906
Svatoš A, Antonchick A, Schneider B. Determination of brassinosteroids in the sub-femtomolar range using dansyl-3-aminophenylboronate derivatization and electrospray mass spectrometry. Rapid Commun Mass Spectrom, 2004, 18:816–821
Swaczynová J, Novák O, Hauserová E, et al. New techniques for the estimation of naturally occurring brassinosteroids. J Plant Growth Regul, 2007, 26:1–14
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Huo, F., Bai, Y. & Liu, H. Fragmentation study of two brassinolides by ion trap tandem mass spectrometry. Chin. Sci. Bull. 55, 2219–2224 (2010). https://doi.org/10.1007/s11434-010-3191-7
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DOI: https://doi.org/10.1007/s11434-010-3191-7