Skip to main content
SpringerLink
Log in

Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hansen G, Shillito R D, Chilton M D. T-strand integration in maize protoplasts after codelivery of a T-DNA substrate and virulence genes. Proc Natl Acad Sci USA, 1997, 94: 11726–11730

    Article  PubMed  CAS  Google Scholar 

  2. Srivastava V, Ow D W. Single-copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct. Plant Mol Biol, 2001, 46: 561–566

    Article  PubMed  CAS  Google Scholar 

  3. Frame B R, Shou H, Chikwamba R K, Zhang Z, Xiang C, Fonger T M, Pegg S E, Li B, Nettleton D S, Pei D, Wang K. Agrobacterium tumefaciens mediated transformation of maize embryos using a standard binary vector system. Plant Physiol, 2002, 129: 13–22

    Article  PubMed  CAS  Google Scholar 

  4. De Cleene M, De Ley J. The host range of infectious hairy root. Bot Rev, 1981, 47: 147–193

    Google Scholar 

  5. Strobel G A, Nachmias, A. Agrobacterium rhizogenes: A root inducing bacterium. In: Davis T D, Haissig B E, Sankhla N, eds. Adventitious Root Formation in Cuttings. Oregon: Dioscorides Press, 1988. 284–288

    Google Scholar 

  6. Porter J R. Host range and implications of plant infection by Agrobacterium rhizogenes. CRC Crit Rev Plant Sci, 1991, 10(4): 387–421

    Article  Google Scholar 

  7. McAfee B J, White E E, Pelcher L E, Lapp M S. Root induction in pine (Pinus) and larch (Larix) ssp. using Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult, 1993, 34(1): 53–62

    Article  Google Scholar 

  8. Hatta M, Beyl C A, Garton S, Diner A M. Induction of roots on jujube softwood cuttings using Agrobacterium rhizogenes. J Hortic Sci Biotechnol, 1996, 71(6): 881–886

    Google Scholar 

  9. White F F, Taylor B H, Huffman G D, Gordon M P, Nester, E W. Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol, 1985, 164: 33–44

    PubMed  CAS  Google Scholar 

  10. Tepfer D. Transformation of several species of higher plants by Agrobacterium rhizogenes: Sexual transmission of the transformed genotype and phenotype. Cell, 1984, 37: 959–967

    Article  PubMed  CAS  Google Scholar 

  11. Bassil N V, Proebsting W M, Moore L W, Lightfoot D A. Propagation of hazelnut stem cuttings using Agrobacterium rhizogenes. HortScience, 1991, 26(8): 1058–1060

    Google Scholar 

  12. Hooykaas P J J, Clapwijk P M, Nuti M P, Schilperoort R A, Roersch A. Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacteria and to Rhizobium ex-planta. J Gen Microbiol, 1977, 98: 477–484

    Google Scholar 

  13. Tzfira T, Ben-meir H, Vainstein A, Altman A. High efficient transformation and regeneration of aspen plants through shoot-bud formation in root culture. Plant Cell Rep, 1996, 15: 566–571

    Article  CAS  Google Scholar 

  14. Tzfira T, Yarnitzky O, Vainstein A, Altman A. Agrobacterium rhizogenes-mediated DNA transfer in Pinus halepensis Mill. Plant Cell Rep, 1996, 16: 26–31

    CAS  Google Scholar 

  15. Frame B, Zhang H, Cocciolone S, Sidorenko L, Dietrich C, Pegg S, Zhen S, Schnable P, Wang K. Production of transgenic maize from bombarded Type II callus: Effect of gold particle size and callus morphology on transformation efficiency. In Vitro Cell Dev Biol Plant, 2000, 36: 21–29

    Google Scholar 

  16. Damiano C, Archilletti T, Caboni E, Lauri P, Falasca G, Mariotti D, Ferraiolo, G. Agrobacterium-mediated transformation of almond: In vitro rooting through localised infection of A. rhizogenes w.t. Acta Horticulturae, 1995, 392: 161–169

    CAS  Google Scholar 

  17. Chitton M D, Tepfer D A, Petit A. Agrobacterium rhizogenes inserts T-DNA into the genome of host plant root cells. Nature, 1982, 295: 432–434

    Article  Google Scholar 

  18. Kato Y, Uozumi N, Kimura T, Honda H, Kobayashi T. Enhancement of peroxidase production and extension from horseradish hairy roots by light, NaCl, and peroxidase-adsorption in situ. Plant Tissue Culture Lett, 1991, 8: 158–165

    CAS  Google Scholar 

  19. Uozumi N, Kohketsu K, Kondo O, Honda H, Kobayashi T. Fedbach culture of hairy root using fructose as a carbon source. J Ferment Bioeng, 1991, 72: 457–460

    Article  CAS  Google Scholar 

  20. Flores H E, Hoy M W, Pickard J J. Secondary metabolites from root cultures. Trends Biotechnol, 1987, 5: 64–69

    CAS  Google Scholar 

  21. Ooms G, Karp A, Burrell M M, Twell D, Roberts J. Genetic modification of potato development using Ri T-DNA. Theor Appl Genet, 1985, 70: 440–446

    CAS  Google Scholar 

  22. Lambert C, Tepfer D. Use of Agrobacterium rhizogenes to create chimeric apple trees through genetic grafting. Biotechnology, 1991, 9: 80–83

    Article  Google Scholar 

  23. Uozumi N, Ohatake Y, Nakashimada Y, Morikawa Y, Tanaka N, Kobayashi T. Efficient regeneration from GUS-transformed Ajuga hairy root. J Ferment Bioeng, 1996, 5: 374–378

    Article  Google Scholar 

  24. Aoyama T, Takanami M, Oka A. Signal structure for transcriptional activation in the upstream regions of virulence genes on the hairy root inducing plasmid A4. Nucleic Acids Res, 1989, 17: 8711–8725

    PubMed  CAS  Google Scholar 

  25. Zhang Y, Shen W H. New carrier of plant gene engineering—Agrobacterium rhizogenes Ri plasmid. Chin J Biotechnol (in Chinese), 1989, 5: 173–178

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Life Sciences, Northeast Normal University, Changchun, 130024, China

    Xu Hongwei, Lu Jingmei, Wang Junjie & Wang Xingzhi

  2. Institute of Gene Engineering, Jilin Normal University, Siping, 136000, China

    Xu Hongwei & Zhou Xiaofu

Authors
  1. Xu Hongwei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhou Xiaofu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lu Jingmei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wang Junjie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wang Xingzhi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wang Xingzhi.

Additional information

These authors contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, H., Zhou, X., Lu, J. et al. Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize. SCI CHINA SER C 49, 305–310 (2006). https://doi.org/10.1007/s11427-006-0305-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-006-0305-1

Keywords

Search

Navigation