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Effects of high-energy-pulse-electron beam radiation on biomacromolecules

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Abstract

To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by 60Co gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.

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Authors and Affiliations

  1. School of Life Science and Technology, TongJi University, Shanghai, 200092, China

    Zhu Hong, Xu JingZao, Li ShiQiang, Sun XiaoYu, Yao SiDe & Wang ShiLong

  2. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China

    Yao SiDe

Authors
  1. Zhu Hong
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  2. Xu JingZao
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  3. Li ShiQiang
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  4. Sun XiaoYu
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  5. Yao SiDe
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  6. Wang ShiLong
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Correspondence to Wang ShiLong.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 30570376 and 50673078), the Shanghai Key Fundamental Project (Grant No. 06JC14068) and the Innovation Program of Shanghai Municipal Education Commission (Grant No. 08ZZ21)

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Zhu, H., Xu, J., Li, S. et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules. Sci. China Ser. B-Chem. 51, 86–91 (2008). https://doi.org/10.1007/s11426-008-0017-4

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  • DOI: https://doi.org/10.1007/s11426-008-0017-4

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