Abstract
Ascorbic acid (AA) is well known as a good radical scavenger and antioxidant. Here, the protection effects of AA against free radicals (mainly hydroxyl radicals) caused by low and high linear energy transfer radiation were examined. In this study, cobalt-60 gamma rays and helium ion beams were used as the sources of ionizing radiation. DNA strand breaks of PUC118 plasmid in the presence and absence of AA were monitored using agarose gel electrophoresis. Moreover, the survival rates of Saccharomyces cerevisiae yeast cells irradiated with different AA concentrations in growing medium were analysed using the linear quadratic survival model. Based on the results of DNA strand break experiments, the yield of strand breaks increased with an increase in absorbed dose. In the presence of AA, the yield of strand breaks decreased with an increase in AA concentration. In other words, the greater the additive concentration, the more effective the radiation-protective agent. On the other hand, in the survival rate experiments, the dependence on additive concentration was not similar to that of the DNA experiments. We consider that the main reason for our observations is the difference in additive concentration between the outside and inside of the cell membrane of yeast cells. These results suggest that AA plays an effective role as a radiation-protective agent for DNA and living cells against free radicals formed by radiation.
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References
Dance DR, Christofides S, Maidment ADA, McLean AD, Ng KH (2014) Diagnostic radiology physics. handbook for teachers and students. International Atomic Energy Agency
Nimse SB, Palb D (2015) Free radicals, natural antioxidants, and their reaction mechanisms. Royal Soc Chem 5:27986–28006
Yamamoto T, Kinoshita M, Shinomiya N, Hiroi S, Sugasawa H, Matsushita Y, Majima T, Saitoh D, Seki S (2010) Pretreatment with ascorbic acid prevents lethal gastrointestinal syndrome in mice receiving a massive amount of radiation. J Radiat Res
Yanagisawa A, Michie Iwata F, Akiyama S (2011) Effect of Vitamin C and anti- oxidative nutrition on radiation induced gene expression in Fukushima Nuclear Plant workers—a pilot study. Japanese College of Intravenous Therapy (JCIT)
Bindhumol V, Chitra KC, Mathur PP (2003) Bisphenol A induces reactive oxygen species generation in the liver of male rats. Toxicology 188:117–124
NCBI homepage, https://pubchem.ncbi.nlm.nih.gov/. Last accessed 27 Dec 2018
Verma RS, F., Mhta A, Srivastava T (2007) In vivo chlorpyrifos induced oxidative stress: attenuation by antioxidant vitamins. Pesticide Biochem Physiol 88:191196
Cai L, Koropatnick J, Cherian MG (2001) Roles of vitamin C in radiation-induced DNA damage in presence and absence of copper. Chem Biol Interact 137:75–88
Smith TA, Kirkpatrick DR, Smith S, Smith TK, Pearson T, Kailasam A, Herrmann KZ, Schubert J, Agrawal DK (2017) Radioprotective agents to prevent cellular damage due to ionizing radiation. J Transl 15:232
Yi J, Chen CC, Liu X, Kang Q, Hao L, Huang J, Lu J (2020) Radioprotection of EGCG based on immunoregulatory effect and antioxidant activity against 60-Co γ radiation-induced injury in mice. Food Chem Toxicol 161:175–186
Matuo Y, Nishijima S, Ikeda T, Shimizu K (2008) Radical scavenging effect of naturallyoccuring epigallocatechin gallate against oxidative damage caused by gamma-ray radiation. Radioisotopes
Gudkov SV, Popova NR, Bruskov VI (2015) Radioprotective substances: history, trends and prospects. Biophysics. Pleiades Publishing, Inc.
Xie LW, Cai S, Zhao TS, Li M, Tian Y (2020) Green tea derivative (−)-epigallocatechin3-gallate (EGCG) confers protection against ionizing radiation-induced intestinal epithelial cell death both in vitro and in vivo. Free Radical Biol Med 161:175–186
Brenner DJ (2008) The linear-quadratic model is an appropriate methodology for determining iso-effective doses at large doses per fraction. NIH Public Access
De Mattos JCP, Dantas FJS, Caldeira-de-Araújo A, Moraes MO (2004) Agarose gel electrophoresis system in the classroom. Biochem Mol Biol Educ 32(4):254–257
Rashid RA, Ariffin NA, Dollah N, Rahman WN (2019) Application of linear quadratic ans multi-target model to single and fractionated radiotherapy irradiation. Jurnal Sains Nuklear Malaysia 31(2):23–31
Flickinger JC, Kondziolka D, Lunsford LD (2003) Radiobiological analysis of tissue responses following radiosurgery. Technol Cancer Res Treat 2(2). ISSN 1533-0346
Nickoloff JA, Sharma N, Taylor L (2020) Clustered DNA double-strand breaks: biological effects and relevance to cancer radiotherapy. Genes (Basel) 11(1):99. https://doi.org/10.3390/genes11010099
Ighodaro OM, Akinloye OA (2017) First line defence antioxidants-superoxide dismutase (Sod), catalase (Cat) and glutathione peroxidase (Gpx): their fundamental role in the entire antioxidant defence grid. Alexandria J Med
Narra VR, Howell RW, Sastry KS, Rao DV (1992) Vitamin C as a radioprotector against iodine-131 in vivo. J Nucl Med 34:637–640
Dabbagh HA, Azami F, Farrokhpour H, Chermahini AN (2014) UV-VIS, NMR and FTIR spectra of tautomers of vitamin C. Experimental and DFT calculations, J Chil Chem Soc 59(3):2248–2254
Prescianotto-Baschong C, Riezman F (1998) Morphology of the yeast endocytic pathway. Mol Biol Cell 9:173–189
Hawkins RB (2017) Biophysical models, microdosimetry and the linear quadratic survival relation. Ann Radiation Therapy Oncol
Ballarini F (2010) From DNA radiation damage to cell death: theoretical approaches. J Nucl Acids
Acknowledgements
The authors would like to thank the institute of Scientific and Industrial Research of Osaka University and the facilities of Radioisotope Research Center at the Suita campus of Osaka University.
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Nhan, T.T., Matuo, Y., Abdillah, M., Wicaksono, L.W., Izumi, Y. (2022). Ascorbic Acid as a Radiation-Protective Agent Against Ionizing Radiation. In: Van Toi, V., Nguyen, TH., Long, V.B., Huong, H.T.T. (eds) 8th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2020. IFMBE Proceedings, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-030-75506-5_69
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