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Arbuscular mycorrhizal-like fungi and glomalin-related soil protein drive the distributions of carbon and nitrogen in a large scale

  • Soils, Sec 5 • Soil and Landscape Ecology • Research Article
  • Published:
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Abstract

Purpose

Arbuscular mycorrhizal-like fungi (AM-like fungi) are crucial for ecosystem functioning and soil organic matter (SOM) is an indicator of soil quality. However, the spatial distribution of arbuscular mycorrhizal-like fungi, glomalin-related soil protein (GRSP) and SOM in a large scale is still unclear. The objectives of this study were to investigate the spatial distribution of SOM, arbuscular mycorrhizal-like fungi and GRSP, and reveal the potential relationship among them in a large scale across China.

Materials and methods

Soil samples (different in vegetation type, climate, and soil variables) were collected from 26 sites in a large scale across China. The soil properties including pH, total carbon (TC), total nitrogen (TN), and SOM were determined. Quantitative PCR amplification of the 18S rRNA gene was conducted to evaluate the abundance of arbuscular mycorrhizal-like fungi. The contents of easily extractable GRSP (EE-GRSP), difficultly extractable GRSP (DE-GRSP), and total GRSP (T-GRSP) were measured.

Results and discussion

Arbuscular mycorrhizal-like fungi abundance was significantly affected by the vegetation type and dramatically correlated with the soil TN and mean annual precipitation (MAP). EE-GRSP and DE-GRSP were more associated with the TC and TN content, respectively. The abundance of arbuscular mycorrhizal-like fungi significantly but weakly correlated with the T-GRSP and EE-GRSP. The SOM content positively correlated with the DE-GRSP and T-GRSP. Those results suggested that the arbuscular mycorrhizal-like fungi are a larger contributor to regulating the content of GRSP, which is an important indicator of the soil organic carbon pool.

Conclusions

Our results indicated that arbuscular mycorrhizal-like fungi abundance has a greater contribution to driving the distribution of soil C and N in a large scale by affecting the content of glomalin-related soil protein.

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References

  • Alguacil MM, Diaz G, Torres P, Rodriguez-Caballero G, Roldan A (2019) Host identity and functional traits determine the community composition of the arbuscular mycorrhizal fungi in facultative epiphytic plant species. Fungal Ecol 39:307–315

    Google Scholar 

  • Chen Z, He X, Guo H, Yao X, Chen C (2012) Diversity of arbuscular mycorrhizal fungi in the rhizosphere of three host plants in the farming-pastoral zone, North China. Symbiosis 57:149–160

    Google Scholar 

  • Chen YL, Xu ZW, Xu TL, Veresoglou SD, Yang GW, Chen BD (2017) Nitrogen deposition and precipitation induced phylogenetic clustering of arbuscular mycorrhizal fungal communities. Soil Biol Biochem 115:233–242

    CAS  Google Scholar 

  • Dai J, Hu JL, Li XG, Yang AN, Wang R, Zhang JB, Wong MH (2013) Arbuscular mycorrhizal fungal diversity, external mycelium length, and glomalin-related soil protein content in response to long-term fertilizer management. J Soils Sediments 13:1–11

    CAS  Google Scholar 

  • Espinoza-Victoria D, Quintero-Ramos M, Ferrera-Cerrato R, Bethlenfalvay GJ (1993) Fitting plants to soil through mycorrhizal fungi: plant nutrition in host-endophyte combinations evaluated by the diagnosis and recommendation integrated system. Biol Fert Soils 15:96–102

    CAS  Google Scholar 

  • Fokom R, Adamou S, Teugwa MC, Boyogueno ADB, Nana W (2012) Glomalin related soil protein, carbon, nitrogen and soil aggregate stability as affected by land use variation in the humid forest zone of South Cameroon. Soil Tillage Res 120:69–75

    Google Scholar 

  • Gao C, Kim YC, Zheng Y, Yang W, Chen L, Ji NN, Wan SQ, Guo LD (2015) Increased precipitation, rather than warming exerts strong influence on arbuscular mycorrhizal fungal community in a semiarid steppe ecosystem. Botany 94:459–469

    Google Scholar 

  • Greiner JT, McGlathery KJ, Gunnell J, McKee BA (2013) Seagrass restoration enhances “blue carbon” sequestration in coastal waters. PLoS One 8:e72469

    CAS  Google Scholar 

  • Han S, Zeng LY, Luo XS, Xiong X, Wen SL, Wang BR, Chen WL, Huang QY (2018a) Shifts in Nitrobacter- and Nitrospira-like nitrite-oxidizing bacterial communities under long-term fertilization practices. Soil Biol Biochem 124:118–125

    CAS  Google Scholar 

  • Han S, Li X, Luo X, Shi LW, Chen W, Huang Q (2018b) Nitrite-oxidizing bacteria community composition and diversity are influenced by fertilizer regimes, but are independent of the soil aggregate in acidic subtropical red soil. Front Microbiol 9:885

    CAS  Google Scholar 

  • Hart MM, Reader RJ (2002) Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol 153:335–344

    Google Scholar 

  • Horn S, Hempel S, Verbruggen E, Rillig MC, Caruso T (2017) Linking the community structure of arbuscular mycorrhizal fungi and plants: a story of interdependence? ISME J 11:1400–1411

    Google Scholar 

  • Kumar S, Singh KA, Ghosh P (2018) Distribution of soil organic carbon and glomalin related soil protein in reclaimed coal mine-land chronosequence under tropical condition. Sci Total Environ 625:1341–1350

    CAS  Google Scholar 

  • Lekberg Y, Koide RT, Rohr JR (2007) Role of niche restrictions and dispersal in the composition of arbuscular mycorrhizal fungal communities. J Ecol 95:95–105

    Google Scholar 

  • Lovelock CE, Wright SF, Clark DA, Ruess RW (2004) Soil stocks of glomalin produced by arbuscular mycorrhizal fungi across a tropical rain forest landscape. J Chem Ecol 92:278–287

    CAS  Google Scholar 

  • Lumini E, Orgiazzi A, Borriello R, Bonfante P, Bianciotto V (2010) Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach. Environ Microbiol 12:2165–2179

    CAS  Google Scholar 

  • Paul EA, Clark FE (1989) Soil biology and biochemistry. Academic Press, San Diego, CA

    Google Scholar 

  • Powell JR, Parrent JL, Hart MM, Klironomos JN, Rillig MC, Maherali H (2009) Phylogenetic trait conservation and the evolution of functional trade-offs in arbuscular mycorrhizal fungi. Proc Roy Soc Lond B Biol 276:4237–4245

    Google Scholar 

  • Qiang W, He XL, Wang JJ, Zhao LL (2019) Temporal and spatial variation of arbuscular mycorrhizal fungi under the canopy of Hedysarum scoparium in the northern desert, China. Appl Soil Ecol 136:139–147

    Google Scholar 

  • Rillig MC (2004a) Arbuscular mycorrhizae and terrestrial ecosystem processes. Ecol Lett 7:740–754

    Google Scholar 

  • Rillig MC (2004b) Arbuscular mycorrhizae, glomalin, and soil aggregation. Can J Soil Sci 84:355–363

    Google Scholar 

  • Rillig MC, Wright SF, Nichols KA, Schmidt WF, Torn MS (2001) Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils. Plant Soil 233:167–177

    CAS  Google Scholar 

  • Rillig MC, Maestre FT, Lamit LJ (2003) Microsite differences in fungal hyphal length, glomalin, and soil aggregate stability in semiarid Mediterranean steppes. Soil Biol Biochem 35:1257–1260

    CAS  Google Scholar 

  • Sato K, Suyama Y, Saito M, Sugawara K (2005) A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction–denature gradient gel electrophoresis. Grassl Sci 51:179–181

    CAS  Google Scholar 

  • Selvakumar G, Yi PH, Lee SE, Shagol CC, Han SG, Sa T, Chuang BN (2018) Effects of long-term subcultured arbuscular mycorrhizal fungi on red pepper plant growth and soil glomalin content. Mycobiology 46:122–128

    Google Scholar 

  • Singh PK, Singh M, Tripathi BN (2013) Glomalin: an arbuscular mycorrhizal fungal soil protein. Protoplasma 250:663–669

    CAS  Google Scholar 

  • Singh AK, Rai A, Singh N (2016) Effect of long-term land use systems on fractions of glomalin and soil organic carbon in the indo-Gangetic plain. Geoderma 277:41–50

    CAS  Google Scholar 

  • Singh AK, Rai A, Pandey V, Singh N (2017a) Contribution of glomalin to dissolve organic carbon under different land uses and seasonality in dry tropics. J Environ Manag 192:142–149

    CAS  Google Scholar 

  • Singh AK, Kushwaha M, Rai A, Singh N (2017b) Changes in soil microbial response across year following a wildfire in tropical dry forest. For Ecol Manag 391:458–468

    Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal Symbiosis. Academic press, Cambridge, pp 13–15

    Google Scholar 

  • Smith SE, Barker SJ, Zhu YG (2006) Fast moves in arbuscular mycorrhizal symbiotic signaling. Trends Plant Sci 11:369–371

    CAS  Google Scholar 

  • Staddon PL, Ramsey CB, Ostle N, Ineson P, Fitter AH (2003) Rapid turnover of hyphae of mycorrhizal fungi determined by AMS microanalysis of 14C. Science 5622:1138–1140

    Google Scholar 

  • Treseder KK, Allen MF (2000) Mycorrhizal fungi have a potential role in soil carbon storage under elevated CO2 and nitrogen deposition. New Phytol 147:189–200

    CAS  Google Scholar 

  • Treseder KK, Turner KM (2007) Glomalin in ecosystems. Soil Sci Soc Am J 71:1257–1266

    CAS  Google Scholar 

  • Wang W, Zhong Z, Wang Q, Wang H, Fu Y, He X (2017) Glomalin contributed more to carbon, nutrients in deeper soils, and differently associated with climates and soil properties in vertical profiles. Sci Rep-UK 7:13003

    Google Scholar 

  • Wang Q, Li J, Chen J, Hong H, Lu H, Liu J, Dong Y, Yan C (2018a) Glomalin-related soil protein deposition and carbon sequestration in the old Yellow River delta. Sci Total Environ 625:619–626

    CAS  Google Scholar 

  • Wang Q, Lu H, Chen J, Hong H, Liu J, Li J, Yan C (2018b) Spatial distribution of glomalin-related soil protein and its relationship with sediment carbon sequestration across a mangrove forest. Sci Total Environ 613-614:548–556

    CAS  Google Scholar 

  • Wright SF, Anderson RL (2000) Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Biol Fertil Soils 31:249–253

    CAS  Google Scholar 

  • Wright SF, Upadhyaya A (1996) Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Sci 161:575–586

    CAS  Google Scholar 

  • Wright SF, Upadhyaya A (1998) A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 198:97–107

    CAS  Google Scholar 

  • Wu QS, He XH, Zhou YN, He KP, Sun YH, Cao MQ (2012) Spatial distribution of glomalin-related soil protein and its relationships with root mycorrhizal, soil aggregates, carbohydrates, activity of protease and glucosidase in the rhizosphere of Citrus unshiu. Soil Biol Biochem 45:181–183

    CAS  Google Scholar 

  • Wu QS, Cao MQ, Zou YN, He XH (2014) Direct and indirect effects of glomalin, mycorrhizal hyphae, and roots on aggregate stability in rhizosphere of trifoliate orange. Sci Rep-UK 4:5823

    CAS  Google Scholar 

  • Yang HX, Guo SX, Liu RJ (2015) Characteristics of arbuscular mycorrhizal fungal diversity and functions in saline-alkali land. Chin J Appl Ecol 26:311–320

    CAS  Google Scholar 

  • Zhang Z, Wang Q, Wang H, Nie S, Liang Z (2017) Effects of soil salinity on the content, composition, and ion binding capacity of glomalin-related soil protein (GRSP). Sci Total Environ 581:657–665

    Google Scholar 

  • Zhang J, Cui S, Feng Z (2018) Climatic factors and mulching affect soil salinity dynamics in coastal areas. Chin J Eco-Agric 26:294–302

    Google Scholar 

  • Zhang WW, Wang C, Liu ML, Yu YC (2019) Integrated reclamation of saline soil nitrogen transformation in the hyphosphere by earthworms and arbuscular mycorrhizal fungus. Appl Soil Ecol 135:137–146

    Google Scholar 

  • Zhou J, Xue K, Xie J, Deng Y, Wu L, Cheng X, Fei S, Deng S, He Z, Van Nostrand JD, Luo Y (2012) Microbial mediation of carbon-cycle feedbacks to climate warming. Nat Clim Chang 2:106–110

    CAS  Google Scholar 

  • Zhu C, Ling N, Guo JJ, Wang M, Guo SW, Shen QR (2016) Impacts of fertilization regimes on arbuscular mycorrhizal fungal (AMF) community composition were correlated with organic matter composition in maize rhizosphere soil. Front Microbiol 7:1840

    Google Scholar 

  • Zoble M, Öpik M (2014) Plant and arbuscular mycorrhizal fungal (AMF) communities – which drives which? J Veg Sci 25:1134–1140

    Google Scholar 

Download references

Funding

This study was supported by the National Basic Research Program of China (973, Grant No. 2015CB150504) and the Fundamental Research Funds for the Central Universities (Project Nos.2662015PY016 and 2662015PY116).

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Author notes

  1. Xiang Li and Shun Han contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430010, China

    Xiang Li, Shun Han, Xuesong Luo, Wenli Chen & Qiaoyun Huang

  2. Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430010, China

    Xuesong Luo & Qiaoyun Huang

Authors
  1. Xiang Li
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  2. Shun Han
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Correspondence to Wenli Chen or Qiaoyun Huang.

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Responsible editor: Yuan Ge

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Li, X., Han, S., Luo, X. et al. Arbuscular mycorrhizal-like fungi and glomalin-related soil protein drive the distributions of carbon and nitrogen in a large scale. J Soils Sediments 20, 963–972 (2020). https://doi.org/10.1007/s11368-019-02421-4

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