Abstract
Biogas production in the cold regions of China is hindered by low temperatures, which led to slow lignocellulose biotransformation. Cold-adapted lignocellulose degrading microbial complex community LTF-27 was used to investigate the influence of hydrolysis on biogas production. After 5 days of hydrolysis at 15 ± 1 °C, the hydrolysis conversion rate of the corn straw went up to 22.64%, and the concentration of acetic acid increased to 2596.56 mg/L. The methane production rates of total solids (TS) inoculated by LTF-27 reached 204.72 mL/g, which was higher than the biogas (161.34 mL/g), and the control group (CK) inoculated with cultural solution (121.19 mL/g), the methane production rate of volatile solids (VS) increased by 26.88% and 68.92%, respectively. Parabacteroides, Lysinibacillus, and Citrobacter were the main organisms that were responsible for hydrolysis. While numerous other bacteria genera in the gas-producing phase, Macellibacteroides were the most commonly occurring one. Methanosarcina and Methanobacteriaceae contributed 86.25% and 11.80% of the total Archaea abundance during this phase. This study proves the psychrotrophic LTF-27’s applicability in hydrolysis and biomass gas production in low temperatures.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors would like to thank the financial research support received from the National Key R&D Program of China (2019YFD1100603), National Key Research and Development Program Strategic Key Special Subject of International Science and Technology Innovation Cooperation (2018YFE026602) and National Natural Science Foundation of China (52076034) is gratefully acknowledged.
Funding
National Key R&D Program of China (2019YFD1100603).
National Key Research and Development Program Strategic Key Special Subject of International Science and Technology Innovation Cooperation (2018YFE026602).
National Natural Science Foundation of China (52,076,034).
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Stopira Yannick Benz Boboua: writing (original draft), formal analysis, validation, investigation, and visualization. Chenyang Zhou: conceptualization, methodology, and project administration. Jiachen Li: writing (review and editing), visualization, conceptualization, and methodology. Weishuai Bi: writing (review and editing), visualization, conceptualization, and methodology. Conceptualization, Methodology. Ruxian Wang: conceptualization and methodology. Shengnan Chen: data curation. Guoxiang Zheng: conceptualization, methodology, writing (review and editing), and supervision.
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Boboua, S.Y.B., Zhou, C., Li, J. et al. Augmentation characteristics and microbial community dynamics of low temperature resistant composite strains LTF-27. Environ Sci Pollut Res 29, 35338–35349 (2022). https://doi.org/10.1007/s11356-022-18677-2
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DOI: https://doi.org/10.1007/s11356-022-18677-2