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An aptly industrialized bioprocess for lactic acid production from corn stover using thermotolerant microbial consortia

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Abstract

Chemical pretreatment of lignocellulosic biomass is a critical step in the conversion of lignocellulose to biofuels and biochemical. The main drawback of this pretreatment process is the formation of inhibitors which exhibit combined toxicity to microorganisms and result to low product concentrations and yields. In this study, the selection of microbial consortia by enrichment on hydrolysate of H2SO4-pretreated corn stover (pre-CS) without detoxification has been investigated as an efficient way to develop new strategies for lignocellulose utilization. The analysis of cattle stomach-dervied microbial consortia domesticated to degrade hydrolysate of pre-CS to produce lactic acid (LA) at different temperatures was investigated. Bacterial 16S rRNA gene amplicon sequencing analyses indicated that the three microbial consortia were taxonomically distinct and Enterococcus became dominant at high temperature. The highest glucose consumption rate was observed at 45 °C, while the three microbial consortia showed similar consumption rates of xylose and arabinose. The selected microbial consortia DUT37, DUT45 and DUT47 showed preferable resistances to inhibitors in hydrolysate of pre-CS and abilities of xylose utilization. A batch simultaneous saccharification and fermentation (SSF) process was developed by microbial consortium DUT47 at 47 °C to produce LA from pre-CS under non-detoxified and non-sterile conditions. The LA concentration and yield were 43.73 g/L and 0.50 g/g-corn stover (CS), respectively. Microbial consortium DUT47 has been shown to be suitable for LA production from H2SO4-pretreated corn stover without detoxification due to its thermophilic growth characteristics, robust tolerance of inhibitors, and the simultaneous utilization of glucose and xylose.

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References

  1. Juturu V, Wu JC (2016) Microbial production of lactic acid: the latest development. Crit Rev Biotechnol 36(6):967–977. https://doi.org/10.3109/07388551.2015.1066305

    Article  CAS  PubMed  Google Scholar 

  2. Yuan SF, Hsu TC, Wang CA, Jang MF, Kuo YC, Alper HS, Guo GL, Hwang WS (2018) Production of optically pure l(+)-lactic acid from waste plywood chips using an isolated thermotolerant Enterococcus faecalis SI at a pilot scale. J Ind Microbiol Biotechnol 45(11):961–970. https://doi.org/10.1007/s10295-018-2078-5

    Article  CAS  PubMed  Google Scholar 

  3. Isikgor FH, Becer CR (2015) Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers. Polym Chem 6(25):4497–4559. https://doi.org/10.1039/c5py00263j

    Article  CAS  Google Scholar 

  4. Ahmad A, Banat F, Taher H (2020) A review on the lactic acid fermentation from low-cost renewable materials: recent developments and challenges. Environ Technol Innov 20:101138. https://doi.org/10.1016/j.eti.2020.101138

    Article  CAS  Google Scholar 

  5. Zhang Z, Li Y, Zhang J, Peng N, Liang Y, Zhao S (2020) High-titer lactic acid production by Pediococcus acidilactici PA204 from corn stover through fed-batch simultaneous saccharification and fermentation. Microorganisms. https://doi.org/10.3390/microorganisms8101491

    Article  PubMed  PubMed Central  Google Scholar 

  6. Chen H, Su ZH, Wang Y, Wang BX, Si ZH, Lu J, Su CS, Ren WQ, Chen HD, Cai D, Qin PY (2020) Lactic acid production from pretreated corn stover with recycled streams. Process Biochem 91:132–140. https://doi.org/10.1016/j.procbio.2019.12.004

    Article  CAS  Google Scholar 

  7. Zhao K, Qiao QG, Chu DQ, Gu HQ, Dao TH, Zhang J, Bao J (2013) Simultaneous saccharification and high titer lactic acid fermentation of corn stover using a newly isolated lactic acid bacterium Pediococcus acidilactici DQ2. Bioresour Technol 135:481–489. https://doi.org/10.1016/j.biortech.2012.09.063

    Article  CAS  PubMed  Google Scholar 

  8. Ouyang J, Ma R, Zheng ZJ, Cal C, Zhang M, Jiang T (2013) Open fermentative production of l-lactic acid by Bacillus sp strain NLO1 using lignocellulosic hydrolyzates as low-cost raw material. Bioresour Technol 135:475–480. https://doi.org/10.1016/j.biortech.2012.09.096

    Article  CAS  PubMed  Google Scholar 

  9. Ben Said S, Or D (2017) Synthetic microbial ecology: engineering habitats for modular consortia. Front Microbiol 8:1125. https://doi.org/10.3389/fmicb.2017.01125

    Article  PubMed  PubMed Central  Google Scholar 

  10. Cui FJ, Li YB, Wan CX (2011) Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis. Bioresource Technol 102(2):1831–1836. https://doi.org/10.1016/j.biortech.2010.09.063

    Article  CAS  Google Scholar 

  11. Zhang YX, Vadlani PV (2015) Lactic acid production from biomass-derived sugars via co-fermentation of Lactobacillus brevis and Lactobacillus plantarum. J Biosci Bioeng 119(6):694–699. https://doi.org/10.1016/j.jbiosc.2014.10.027

    Article  CAS  PubMed  Google Scholar 

  12. Sun YQ, Xu ZZ, Zheng YF, Zhou JJ, Xiu ZL (2019) Efficient production of lactic acid from sugarcane molasses by a newly microbial consortium CEE-DL15. Process Biochem 81:132–138. https://doi.org/10.1016/j.procbio.2019.03.022

    Article  CAS  Google Scholar 

  13. Malmierca S, Diez-Antolinez R, Paniagua AI, Martin M (2017) Technoeconomic study of biobutanol AB production. 1. Biomass pretreatment and hydrolysis. Ind Eng Chem Res 56(6):1518–1524. https://doi.org/10.1021/acs.iecr.6b02943

    Article  CAS  Google Scholar 

  14. Sun YQ, Yang Y, Liu HH, Wei CX, Qi WB, Xiu ZL (2020) Simultaneous liquification, saccharification and fermentation of l-lactic acid using aging paddy rice with hull by an isolated high-thermotolerant Enterococcus faecalis DUT1805. Bioprocess Biosyst Eng 43(9):1717–1727. https://doi.org/10.1007/s00449-020-02364-y

    Article  CAS  PubMed  Google Scholar 

  15. Xue C, Zhang X, Wang J, Xiao M, Chen L, Bai F (2017) The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation. Biotechnol Biofuels 10:148. https://doi.org/10.1186/s13068-017-0836-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Baral NR, Slutzky L, Shah A, Ezeji TC, Cornish K, Christy A (2016) Acetone–butanol–ethanol fermentation of corn stover: current production methods, economic viability and commercial use. Fems Microbiol Lett 363(6):fnw033. https://doi.org/10.1093/femsle/fnw033

    Article  CAS  PubMed  Google Scholar 

  17. Gorke B, Stulke J (2008) Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol 6(8):613–624. https://doi.org/10.1038/nrmicro1932

    Article  CAS  PubMed  Google Scholar 

  18. Abdel-Rahman MA, Sonomoto K (2016) Opportunities to overcome the current limitations and challenges for efficient microbial production of optically pure lactic acid. J Biotechnol 236:176–192. https://doi.org/10.1016/j.jbiotec.2016.08.008

    Article  CAS  PubMed  Google Scholar 

  19. Hu JL, Zhang ZT, Lin YX, Zhao SM, Mei YX, Liang YX, Peng N (2015) High-titer lactic acid production from NaOH-pretreated corn stover by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile condition. Bioresour Technol 182:251–257. https://doi.org/10.1016/j.biortech.2015.02.008

    Article  CAS  PubMed  Google Scholar 

  20. Zhang YX, Vadlani PV (2013) d-Lactic acid biosynthesis from biomass-derived sugars via Lactobacillus delbrueckii fermentation. Bioprocess Biosyst Eng 36(12):1897–1904. https://doi.org/10.1007/s00449-013-0965-8

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China [grant numbers 22078043, 21476042].

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Authors and Affiliations

  1. School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, Liaoning, People’s Republic of China

    Yaqin Sun, Xiaoying Li, Chuanxiang Wei, Wenbin Qi & Zhilong Xiu

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  1. Yaqin Sun
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  2. Xiaoying Li
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  3. Chuanxiang Wei
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  4. Wenbin Qi
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  5. Zhilong Xiu
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Correspondence to Zhilong Xiu.

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Sun, Y., Li, X., Wei, C. et al. An aptly industrialized bioprocess for lactic acid production from corn stover using thermotolerant microbial consortia. Bioprocess Biosyst Eng 44, 2445–2454 (2021). https://doi.org/10.1007/s00449-021-02616-5

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