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Revealing the correlation of biomethane generation, DOM fluorescence, and microbial community in the mesophilic co-digestion of chicken manure and sheep manure at different mixture ratio

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Abstract

Batch co-digestion tests of chicken manure (CM) and sheep manure (SM) at different ratio (Rs/c) were conducted under mesophilic condition (35 °C). Batch kinetic analysis of bioCH4 production, excitation-emission matrix (EEM) fluorescence of dissolved organic matter (DOM), and microbial community were investigated. The well-fitted modified Gompertz model (R2, 0.98–0.99) resulted that the co-digestion markedly improved the methane production rate and shortened the lag phase time. The highest bioCH4 yield of 219.67 mL/gVSadd and maximum production rate of 0.378 mL/gVSadd/h were obtained at an optimum Rs/c of 0.4. Additionally, a significant variation of DOM was detected at the Rs/c of 0.4 with a consistent degradation of soluble microbial byproduct-like and protein-like organics. The positive synergy effects of co-digestion conspicuously enhanced the bioCH4 production efficiency. FI370 and NADH were significantly correlated to Rs/c (p < 0.05). Moreover, the correlations among process indicator, EEM-peaks and different environmental parameters were evaluated by Pearson correlation analysis. The high diversity of acetoclastic methanogens and hydrogenotrophic methanogens in the co-digestion improved the stability of process.

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Abbreviations

CM:

Chicken manure

SM:

Sheep manure

Rs/c :

Volatile solid ratio of sheep manure to chicken manure

SCOD:

Dissolved chemical oxygen demand

TCOD:

Total chemical oxygen demand

DOM:

Dissolved organic matter

EEM:

Three-dimensional excitation-emission matrix

Em:

Emission

Ex:

Excitation

TS:

Total solid

VS:

Volatile solid

SMP:

Soluble microbial byproduct-like materials

BIX:

The biological index

HIX:

The humification index

NADH:

Nicotinamide adenine dinucleotide (the reduced form)

NAD+ :

Nicotinamide adenine dinucleotide (the oxidation form)

TAN:

Total ammonia nitrogen

FA:

Free ammonia

FRI:

Fluorescence regional integration

TP:

Total phosphorus

Ss:

The seed sludge

Cs:

The co-digestion sludge

Svs:

The sludge setting in starvation

AS:

Anaerobic sludge

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Funding

The authors’ research is supported by the National Natural Science Foundation of China (Grant No. 51608304 and Grant No. U1806216) and Young Scholars Program of Shandong University (2018WLJH53). Research Fund of Tianjin Key Laboratory of Aquatic Science and Technology (TJKLAST-ZD-2017-04) and Research Fund of Jiangsu Key Laboratory of Anaerobic Biotechnology (JKLAB201702) were also partly supported this work. “The Fundamental Research Funds of Shandong University” and China Postdoctoral Science Foundation (2017M622209). also supported this study.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, China–America CRC for Environment & Health of Shandong Province, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, People’s Republic of China

    Liuying Song, Dunjie Li, Hongli Fang, Xiangyunong Cao, Rutao Liu & Qigui Niu

  2. Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Qigui Niu

  3. Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai, 980-8579, Japan

    Yu-You Li

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  1. Liuying Song
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  2. Dunjie Li
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  3. Hongli Fang
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  4. Xiangyunong Cao
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  5. Rutao Liu
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Correspondence to Qigui Niu.

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Highlights

• Co-digestion of different ratio of CM and SM (Rs/c) were conducted in batch tests.

• Kinetic analysis of co-digestion of CM and SM were conducted under different Rs/c.

• Maximum bioCH4 production rate of 0.378 mL/gVSadd/h was achieved at Rs/c of 0.4.

• Co-digestion of Rs/c of 0.4 obtained a significant DOM variation following time by EEM.

• Microbial community structure varied greatly after feeding with co-substrate.

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Song, L., Li, D., Fang, H. et al. Revealing the correlation of biomethane generation, DOM fluorescence, and microbial community in the mesophilic co-digestion of chicken manure and sheep manure at different mixture ratio. Environ Sci Pollut Res 26, 19411–19424 (2019). https://doi.org/10.1007/s11356-019-05175-1

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