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Influence of Spirulina platensis and ascorbic acid on amikacin-induced nephrotoxicity in rabbits

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Abstract

The current study was performed to investigate the nephroprotective efficacy of Spirulina platensis (SP) and the possible benefits of combining SP and ascorbic acid (AA) in protecting against amikacin (AMK)-induced nephrotoxicity in rabbits. Forty-two male New Zealand rabbits were allocated to seven equal groups, receiving (I) normal saline as negative controls, (II) oral SP (500 mg/kg body weight), (III) oral AA (20 mg/kg bw), (IV) intramuscular AMK injection (100 mg/kg bw), (V) AMK plus SP, (VI) AMK plus AA, or (VII) AMK plus SP and AA at the aforementioned doses. The treatments were given once/day for 7 days. Data analysis showed that in comparison to the control group, AMK-intoxicated rabbits showed significant increases (p ≤ 0.05) in serum concentrations of creatinine, uric acid, and urea, as well as renal tissue concentrations of tumor necrosis factor-α [TNF-α], malondialdehyde [MDA], and nitric oxide [NO]. Moreover, significant (p ≤ 0.05) reductions in renal glutathione concentration, antioxidant enzymatic activities (catalase, glutathione peroxidase, and superoxide dismutase), and total antioxidant capacity were noted following AMK intoxication. Treatment by SP ameliorated most of the aforementioned AMK-induced alterations. Although treatment with AA significantly reduced the renal tissue MDA, NO, and TNF-α concentrations, it was not associated with significant ameliorations of AMK-induced changes in the serum concentrations of renal function markers or renal tissue antioxidant parameters. The nephroprotective effects of SP-AA combination were more potent than SP alone in several parameters. In conclusion, SP alone or in combination with AA minimized the nephrotoxic effects of AMK through their antioxidant and anti-inflammatory activities.

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Abbreviations

AA:

ascorbic acid

AMK:

amikacin

CAT:

catalase

GPx:

glutathione peroxidase

GSH:

reduced glutathione

MDA:

malondialdehyde

NO:

nitric oxide

SP :

Spirulina platensis

SOD:

superoxide dismutase

TAC:

total antioxidant capacity

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Authors and Affiliations

  1. Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

    Mohamed M. Abdel-Daim

  2. Department of Poultry and Rabbit Medicine, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

    Amira Ahmed

  3. Department of Pharmacy, University of Agriculture, Faisalabad, Pakistan

    Hira Ijaz

  4. Faculty of Medicine, Ain Shams University, Ramsis St, 11566, Abbasia, Cairo, Egypt

    Abdelrahman Ibrahim Abushouk

  5. Faculty of Medicine, Zagazig University, Zagazig, El-Sharkia, Egypt

    Hussien Ahmed & Ahmed Negida

  6. Chrono-Environnement Laboratory, UMR CNRS 6249, Bourgogne Franche-Comté University, 25030, Besançon Cedex, France

    Lotfi Aleya

  7. Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, Oradea, Romania

    Simona G. Bungau

Authors
  1. Mohamed M. Abdel-Daim
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  2. Amira Ahmed
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  3. Hira Ijaz
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  4. Abdelrahman Ibrahim Abushouk
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  5. Hussien Ahmed
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  6. Ahmed Negida
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  7. Lotfi Aleya
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  8. Simona G. Bungau
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Corresponding authors

Correspondence to Mohamed M. Abdel-Daim or Abdelrahman Ibrahim Abushouk.

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All animal care procedures were performed following the guidelines of the European Communities Council Directive (86/609/EEC) and were approved by the local ethical research committee at Suez Canal University (Ismailia, Egypt).

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Abdel-Daim, M.M., Ahmed, A., Ijaz, H. et al. Influence of Spirulina platensis and ascorbic acid on amikacin-induced nephrotoxicity in rabbits. Environ Sci Pollut Res 26, 8080–8086 (2019). https://doi.org/10.1007/s11356-019-04249-4

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