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Genetic damage in Rhinella marina populations in habitats affected by agriculture in the middle region of the Sinú River, Colombia

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Abstract

Contamination with pesticide residues affects the environmental health of agroecosystems, especially the amphibian fauna that lives in these environments. The objective of the present study was to determine pesticides concentrations in sediments of agroecosystems and to evaluate genetic damage in Rhinella marina populations living in these zones. A total of 91 individuals were collected, 51 in the group exposed in different areas of the middle region of the Sinú River (Irrigation District of Mocari 16, Irrigation District of Aguas Negras 21, Irrigation District of Cerete 14) and 40 in a control group; at the same time, 36 subsamples of sediments were taken at each sampled station to determine pesticides organochlorine by means of chromatography coupled with ISQ Thermo Scientific mass spectrometer. The micronucleus test was applied in erythrocytes of the individuals collected. Results showed the presence of persistent organochlorine pesticides (POPs) in the sediment samples (p,p′-DDT, p,p′-DDE, and p,p′-DDD) of agricultural soils. Two individuals were registered with abnormalities in their limbs at the Mocari station, representing 12.5% of the morphological malformations to this sector. Micronucleus analysis revealed statistically significant genetic damage in exposed individuals (Mocari 9.87 ± 5.1, Cerete 7.7 ± 1.7, Aguas Negras 5.6 ± 3.6) with respect to the control group (2.4 ± 1.9) (p < 0.05). Spearman correlation analysis revealed a positive association between genetic damage and POP concentrations (p < 0.05). In addition, cellular alterations such as nuclear buds, and pyknosis (cell death), were statistically significant in the exposed group compared to the control group (p < 0.05). This study suggests that there is evidence for morphological and genotoxic effects in R. marina populations inhabiting areas influenced by agriculture, possibly associated with the presence of p,p′-DDT, p,p′-DDD, and p,p′-DDE.

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Acknowledgements

The authors wish to thank the University of Cordoba, Montería-Colombia, as well as Water, Applied, and Environmental Chemistry Group, Laboratory of Toxicology and Environmental Management, the University of Cordoba, Montería-Colombia, and biologist Mauricio Begambre for their continuous dedication and support during this study.

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Authors and Affiliations

  1. Laboratory Toxicology and Environmental Management, University of Córdoba, Cra 6 no. 76-103, Monteria, 354, Colombia

    Ángel Cruz-Esquivel

  2. Biodiversity Group, University of Córdoba, Cra 6 no. 76-103, Monteria, 354, Colombia

    Jose Viloria-Rivas

  3. Water, Applied, and Environmental Chemistry Group, University of Córdoba, Monteria, Colombia

    Jose Marrugo-Negrete

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  1. Ángel Cruz-Esquivel
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  2. Jose Viloria-Rivas
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  3. Jose Marrugo-Negrete
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Correspondence to Ángel Cruz-Esquivel.

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Cruz-Esquivel, Á., Viloria-Rivas, J. & Marrugo-Negrete, J. Genetic damage in Rhinella marina populations in habitats affected by agriculture in the middle region of the Sinú River, Colombia. Environ Sci Pollut Res 24, 27392–27401 (2017). https://doi.org/10.1007/s11356-017-0134-8

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