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Differential Patterns of Accumulation and Retention of Dietary Trace Elements Associated With Coal Ash During Larval Development and Metamorphosis of an Amphibian

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Abstract

We performed an experiment in which larval gray tree frogs (Hyla chrysoscelis) were raised through metamorphosis on diets increased with a suite of elements associated with coal combustion residues (silver [Ag], arsenic [As], cadmium [Cd], chromium [Cr], copper [Cu], mercury [Hg], lead [Pb], selenium [Se], vanadium [V], and zinc [Zn]) at “low” and “high” concentrations. We quantified accumulation of metals at three life stages (mid-larval development, initiation of metamorphosis, and completion of metamorphosis) as well as effects on survival, metabolic rate, size at metamorphosis, and duration and loss of weight during metamorphosis. Most elements were accumulated in a dose-dependent pattern by some or all life stages, although this was not the case for Hg. For most elements, larval body burdens exceeded those of later life stages in some or all treatments (control, low, or high). However for Se, As, and Hg, body burdens in control and low concentrations were increased in later compared with earlier life stages. A lack of dose-dependent accumulation of Hg suggests that the presence of high concentrations of other elements (possibly Se) either inhibited accumulation or increased depuration of Hg. The duration of metamorphosis (forelimb emergence through tail resorption) was lengthened in individuals exposed to the highest concentrations of elements, but there were no other statistically significant biological effects. This study shows that patterns of accumulation and possibly depuration of metals and trace elements are complex in animals possessing complex life cycles. Further study is required to determine specific interactions affecting these patterns, in particular which elements may be responsible for affecting accumulation or retention of Hg when organisms are exposed to complex mixtures of elements.

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Acknowledgments

P. Conrad was supported by the National Science Foundation Research Experience for Undergraduates Program 0754609 administered by Maryland Sea Grant. This is contribution No. 4810 of the University of Maryland Center for Environmental Science.

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Authors and Affiliations

  1. Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science, Solomons, MD, 20688, USA

    Andrew Heyes, Christopher L. Rowe & Phillip Conrad

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  1. Andrew Heyes
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  2. Christopher L. Rowe
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  3. Phillip Conrad
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Correspondence to Andrew Heyes.

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Heyes, A., Rowe, C.L. & Conrad, P. Differential Patterns of Accumulation and Retention of Dietary Trace Elements Associated With Coal Ash During Larval Development and Metamorphosis of an Amphibian. Arch Environ Contam Toxicol 66, 78–85 (2014). https://doi.org/10.1007/s00244-013-9957-6

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  • DOI: https://doi.org/10.1007/s00244-013-9957-6

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