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A combination of He-Ne laser irradiation and exogenous NO application efficiently protect wheat seedling from oxidative stress caused by elevated UV-B stress

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Abstract

The elevated ultraviolet-B (UV-B) stress induces the accumulation of a variety of intracellular reactive oxygen species (ROS), which seems to cause oxidative stress for plants. To date, very little work has been done to evaluate the biological effects of a combined treatment with He-Ne laser irradiation and exogenous nitric oxide (NO) application on oxidative stress resulting from UV-B radiation. Thus, our study investigated the effects of a combination with He-Ne laser irradiation and exogenous NO treatment on oxidative damages in wheat seedlings under elevated UV-B stress. Our data showed that the reductions in ROS levels, membrane damage parameters, while the increments in antioxidant contents and antioxidant enzyme activity caused by a combination with He-Ne laser and exogenous NO treatment were greater than those of each individual treatment. Furthermore, these treatments had a similar effect on transcriptional activities of plant antioxidant enzymes. This implied that the protective effects of a combination with He-Ne laser irradiation and exogenous NO treatment on oxidative stress resulting from UV-B radiation was more efficient than each individual treatment with He-Ne laser or NO molecule. Our findings might provide beneficial theoretical references for identifying some effective new pathways for plant UV-B protection.

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Acknowledgments

This research was supported by the Shanxi Province Nature Science Foundation (Grant No. 2014011028-5) and the Nature Science Foundation of Shanxi Normal University (Grant No. ZR1414). The authors are grateful to editors and reviewers for helpful comments on earlier versions of the manuscript.

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Authors and Affiliations

  1. Analysis and Testing Center, Shanxi Normal University, Linfen, 041004, China

    Yongfeng Li

  2. Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041004, China

    Yongfeng Li, Limei Gao & Rong Han

  3. College of Life Science, Shanxi Normal University, Linfen, 041004, China

    Limei Gao & Rong Han

Authors
  1. Yongfeng Li
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  2. Limei Gao
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  3. Rong Han
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Correspondence to Limei Gao or Rong Han.

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Responsible editor: Philippe Garrigues

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Li, Y., Gao, L. & Han, R. A combination of He-Ne laser irradiation and exogenous NO application efficiently protect wheat seedling from oxidative stress caused by elevated UV-B stress. Environ Sci Pollut Res 23, 23675–23682 (2016). https://doi.org/10.1007/s11356-016-7567-3

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  • DOI: https://doi.org/10.1007/s11356-016-7567-3

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