Abstract
UV-B acclimation effects and UV-B damage repair induced by a 632.8-nm He-Ne laser were investigated in Arabidopsis thaliana plants in response to supplementary UV-B stress. There was an increasing trend in growth parameters in the combination-treated plants with He-Ne laser and UV-B light compared to those stressed with enhanced UV-B light alone during different developmental stages of plants. The photosynthetic efficiency (Pn) and survival rates of seedlings were significantly higher in the combination treatments than UV-B stress alone. The expression of UVR8, phytochrome B (PhyB), and their mediated signal responsive genes such as COP1, HY5, and CHS were also significantly upregulated in plants with the laser irradiation compared with other groups without the laser. Levels of flavonol accumulation in leaves and capsule yield of He-Ne laser-treated plants were increased. The phyB-9 mutants were more sensitive to enhanced UV-B stress and had no obvious improvements in plant phenotypic development and physiological damage caused by enhanced UV-B stress after He-Ne laser irradiation. Our results suggested that UVR8 and its mediated signaling pathway via interaction with COP1 can be induced by He-Ne laser, and these processes were dependent on cytoplasmic PhyB levels in plant cells, which might be one of the most important mechanisms of He-Ne laser on UV-B protection and UV-B damage repair. These current data have also elucidated that the biostimulatory effects of He-Ne laser on Arabidopsis thaliana plants would happen not only during the early growth stage but also during the entire late developmental stage.
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References
Alonso R, Berli FJ, Fontana A, Piccoli P, Bottini R (2016) Malbec grape (Vitis vinifera L.) responses to the environment: berry phenolics as influenced by solar UV-B, water deficit and sprayed abscisic acid. Plant Physiol Biochem 109:84–90. https://doi.org/10.1016/j.plaphy.2016.09.007
Björn LO (2015) On the history of phyto-photo UV science (not to be left in skoto toto and silence). Plant Physiol Biochem 93:3–8. https://doi.org/10.1016/j.plaphy.2014.09.015
Brown BA, Headland LR, Jenkins GI (2009) UV-B action spectrum for UVR8-mediated HY5 transcript accumulation in Arabidopsis. Photochem Photobiol 85(5):1147–1155. https://doi.org/10.1111/j.1751-1097.2009.00579.x
Chen YP (2008) Isatis indigotica seedlings derived from laser stimulated seeds showed improved resistance to elevated UV-B. Plant Growth Regul 55(1):73–79. https://doi.org/10.1007/s10725-008-9258-7
Chen PX, Chen HZ, Han R (2016) Effects of enhanced ultraviolet-B radiation on chromosomes and microtubule arrays in wheat roots. Int J Agric Biol 18(03):649–652. 10.17957/IJAB/15.0148
Darré M, Valerga L, Araque LCO, Lemoine ML, Demkura PV, Vicente AR, Concellón A (2017) Role of UV-B irradiation dose and intensity on color retention and antioxidant elicitation in broccoli florets (Brassica oleracea var. Italica). Postharvest Biol Technol 128:76–82. https://doi.org/10.1016/j.postharvbio.2017.02.003
Fasano R, Gonzalez N, Tosco A, Dal Piaz F, Docimo T, Serrano R, Grillo S, Leone A, Inzé D (2014) Role of Arabidopsis UV resistance locus 8 in plant growth reduction under osmotic stress and low levels of UV-B. Mol Plant 7(5):773–791. https://doi.org/10.1093/mp/ssu002
Favory JJ, Stec A, Gruber H, Rizzini L, Oravecz A, Funk M, Albert A, Cloix C, Jenkins GI, Oakeley EJ, Seidlitz HK, Nagy F, Ulm R (2009) Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. EMBO J 28(5):591–601. https://doi.org/10.1038/emboj.2009.4
Formica-Oliveira AC, Martínez-Hernández GB, Díaz-López V, Artés F, Artés-Hernández F (2017) Effects of UV-B and UV-C combination on phenolic compounds biosynthesis in fresh-cut carrots. Postharvest Biol Technol 127:99–104. https://doi.org/10.1016/j.postharvbio.2016.12.010
Gao LM, Li YF, Han R (2015) He-Ne laser preillumination improves the resistance of tall fescue (Festuca arundinacea Schreb.) seedlings to high saline conditions. Protoplasma 252(4):1135–1148. https://doi.org/10.1007/s00709-014-0748-3
Gao LM, Li YF, Han R (2016) Cell wall reconstruction and DNA damage repair play a key role in the improved salt tolerance effects of He-Ne laser irradiation in tall fescue seedlings. Biosci Biotechnol Biochem 80(4):682–693. https://doi.org/10.1080/09168451.2015.1101335
Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot 60(12):3531–3544. https://doi.org/10.1093/jxb/erp194
Hayes S, Sharma A, Fraser DP, Trevisan M, Cragg-Barber CK, Tavridou E, Fankhauser C, Jenkins GI, Franklin KA (2017) UV-B perceived by the UVR8 photoreceptor inhibits plant thermomorphogenesis. Curr Biol 27(1):120–127. https://doi.org/10.1016/j.cub.2016.11.004
Heijde M, Ulm R (2012) UV-B photoreceptor-mediated signaling in plants. Trends Plant Sci 17(4):230–237. https://doi.org/10.1016/j.tplants.2012.01.007
Huang X, Ouyang X, Yang P, Lau OS, Li G, Li J, Chen H, Deng XW (2012) Arabidopsis FHY3 and HY5 positively mediate induction of COP1 transcription in response to photomorphogenic UV-B light. Plant Cell 24(11):4590–4606. https://doi.org/10.1105/tpc.112.103994
Huang X, Ouyang X, Yang P, Lau OS, Chen L, Wei N, Deng XW (2013) Conversion from CUL4-based COP1–SPA E3 apparatus to UVR8–COP1–SPA complexes underlies a distinct biochemical function of COP1 under UV-B. Proc Natl Acad Sci U S A 110(41):16669–16674. https://doi.org/10.1073/pnas.1316622110
Jenkins GI (2009) Signal transduction in responses to UV-B radiation. Annu Rev Plant Biol 60(1):407–431. https://doi.org/10.1146/annurev.arplant.59.032607.092953
Jenkins GI (2014) Structure and function of the UV-B photoreceptor UVR8. Curr Opin Struct Biol 29:52–57. https://doi.org/10.1016/j.sbi.2014.09.004
Kataria S, Jajoo A, Guruprasad KN (2014) Impact of increasing ultraviolet-B (UV-B) radiation on photosynthetic processes. J Photochem Photobiol B Biol 137:55–66
Khudyakova AY, Kreslavski VD, Shirshikova GN, Zharmukhamedov SK, Kosobryukhov AA, Allakhverdiev SI (2017) Resistance of Arabidopsis thaliana L. photosynthetic apparatus to UV-B is reduced by deficit of phytochromes B and A. J Photochem Photobiol B Biol 169:41–46. https://doi.org/10.1016/j.jphotobiol.2017.02.024
Kim BC, Tennessen DJ, Last RL (1998) UV-B-induced photomorphogenesis in Arabidopsis thaliana. Plant J 15(5):667–674. https://doi.org/10.1046/j.1365-313x.1998.00246.x
Kim JH, Lee HJ, Jung JH, Lee S, Park CM (2017) HOS1 facilitates the phytochrome B-mediated inhibition of PIF4 function during hypocotyls growth in Arabidopsis. Mol Plant 10(2):274–284. https://doi.org/10.1016/j.molp.2016.11.009
Kreslavski VD, Shirshikova GN, Lyubimov VY, Shmarev AN, Boutanaev AM, Kosobryukhov AA, Schmitt FJ, Friedrich T, Allakhverdiev SI (2013) Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A. J Photochem Photobiol B Biol 127:229–236. https://doi.org/10.1016/j.jphotobiol.2013.08.008
Li YF, Gao LM, Han R (2016) A combination of He-Ne laser irradiation and exogenous NO application efficiently protect wheat seedling from oxidative stress caused by elevated UV-B stress. Environ Sci Pollut Res 23(23):23675–23682. https://doi.org/10.1007/s11356-016-7567-3
Li YF, Gao LM, Han R (2017) He-Ne laser illumination ameliorates photochemical impairment in ultraviolet-B stressed wheat seedlings via detoxifying ROS cytotoxicity. Russ J Plant Physiol 64(5):766–775. https://doi.org/10.1134/S1021443717050041
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262
Morales LO, Brosché M, Vainonen J, Jenkins GI, Wargent JJ, Sipari N, Strid Å, Lindfors AV, Tegelberg R, Aphalo PJ (2013) Multiple roles for UV RESISTANCE LOCUS 8 in regulating gene expression and metabolite accumulation in Arabidopsis under solar UV radiation. Plant Physiol 161(2):744–759. https://doi.org/10.1104/pp.112.211375
Perveen R, Jamil Y, Ashraf M, Ali Q, Iabal M, Ahmad MR (2011) He-Ne laser induced improvement in biochemical, physiological, growth and yield characteristics in sunflower (Helianthus annuus L.) Photochem Photobiol 87(6):1453–1463. https://doi.org/10.1111/j.1751-1097.2011.00974.x
Qi Z, Yue M, Wang XL (2000) Laser pretreatment protects cells of broad bean from UV-B radiation damage. J Photochem Photobiol B Biol 59:33–37
Qi Z, Yue M, Wang XL (2002) The damage repair of He-Ne laser on plants exposed to different intensities of ultraviolet-B radiation. Photochem Photobiol 75(6):680–686. https://doi.org/10.1562/0031-8655(2002)075<0680:TDRROH>2.0.CO;2
Tripathi DK, Singh S, Singh VP, Prasad SM, Dubey NK, Chauhan DK (2017) Silicon nanoparticles more effectively alleviated UV-B stress than silicon in wheat (Triticum aestivum) seedlings. Plant Physiol Biochem 110:70–81. https://doi.org/10.1016/j.plaphy.2016.06.026
Verdaguer D, Jansen MAK, Llorens L, Morales LO, Neugart S (2017) UV-A radiation effects on higher plants: exploring the known unknown. Plant Sci 255:72–81. https://doi.org/10.1016/j.plantsci.2016.11.014
Wobbe L, Bassi R, Kruse O (2016) Multi-level light capture control in plants and green algae. Trends Plant Sci 21(1):55–68. https://doi.org/10.1016/j.tplants.2015.10.004
Wu D, Hu Q, Yan Z, Chen W, Yan GY, Huang X, Zhang J, Yang PY, Deng HT, Wang JW, Deng XW, Shi YG (2012) Structural basis of ultraviolet-B perception by UVR8. Nature 484:214–219
Xie YJ, Zhang W, Duan XL, Dai C, Zhang YH, Cui WT, Wang R, Shen WB (2015) Hydrogen-rich water-alleviated ultraviolet-B-triggered oxidative damage is partially associated with the manipulation of the metabolism of (iso) flavonoids and antioxidant defence in Medicago sativa. Funct Plant Biol 42:1141–1157
Yang LY, Han R, Sun Y (2012) Damage repair effect of He-Ne laser on wheat exposed to enhanced ultraviolet-B radiation. Plant Physiol Biochem 57:218–221. https://doi.org/10.1016/j.plaphy.2012.06.003
Yin RH (2017) Cooling down thermomorphogenesis by UV-B signaling. Trends Plant Sci 22(6):447–449. https://doi.org/10.1016/j.tplants.2017.04.003
Yin RH, Ulm R (2017) How plants cope with UV-B: from perception to response. Curr Opin Plant Biol 37:42–48. https://doi.org/10.1016/j.pbi.2017.03.013
Yokawa K, Baluška F (2015) Pectins, ROS homeostasis and UV-B responses in plant roots. Phytochemistry 112:80–83. https://doi.org/10.1016/j.phytochem.2014.08.016
Zhao C, Mao K, You CX, Zhao XY, Wang SH, Li YY, Hao YJ (2016) Molecular cloning and functional analysis of a UV-B photoreceptor gene, MdUVR8 from apple. Plant Sci 247:115–126. https://doi.org/10.1016/j.plantsci.2016.03.006
Zhu Y, Ge XM, Wu MM, Li X, He JM (2014) The role and interactions of cytosolic alkalization and hydrogen peroxide in ultraviolet B-induced stomatal closure in Arabidopsis. Plant Sci 215-216:84–90. https://doi.org/10.1016/j.plantsci.2013.11.010
Acknowledgements
We thank Dr. Shaoying Zhang (Oregon State University, USA) for critical reading and editing the manuscript, and we acknowledge the foundation of Higher School Science and Technology Innovation Project of Shanxi Province (Grant no. 20161106) and Cultivation Project of Youth Backbone Teacher of Shanxi Normal University.
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Gao, L., Li, Y., Shen, Z. et al. Responses of He-Ne laser on agronomic traits and the crosstalk between UVR8 signaling and phytochrome B signaling pathway in Arabidopsis thaliana subjected to supplementary ultraviolet-B (UV-B) stress. Protoplasma 255, 761–771 (2018). https://doi.org/10.1007/s00709-017-1184-y
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DOI: https://doi.org/10.1007/s00709-017-1184-y