Summary
The appearance of surface active compounds in the culture broths grown on hydrocarbons can be easily monitored by measuring the surface tension of the cell free broth. Using three bacterial strains it was found that yields of the biosurfactants were maximum at the time when lowest surface tension values were recorded. Thus reduction of surface tension of the medium is a rapid method for assay of maximum biosurfactant formation prior to their actual isolation.
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Pruthi, V., Singh Cameotra, S. Rapid method for monitoring maximum biosurfactant production obtained by acetone precipitation. Biotechnol Tech 9, 271–276 (1995). https://doi.org/10.1007/BF00151574
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DOI: https://doi.org/10.1007/BF00151574