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Biodegradation of bisphenols with immobilized laccase or tyrosinase on polyacrylonitrile beads

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Abstract

The biodegradation of waters polluted by some bisphenols, endowed with endocrine activity, has been studied by means of laccase or tyrosinase immobilized on polyacrylonitrile (PAN) beads. Bisphenol A (BPA), Bisphenol B (BPB), Bisphenol F (BPF) and Tetrachlorobisphenol A (TCBPA) have been used. The laccase-PAN beads system has been characterized as a function of pH, temperature and substrate concentration. The biochemical parameters so obtained have been compared with those of the free enzyme to evidence the modification induced by the immobilization process. Once characterized, the laccase-PAN beads have been employed in a fluidized bed reactor to determine for each of the four bisphenols the degradation rate constant (k); the τ50, i.e., the time to obtain the 50% of degradation, and the removal efficiency (RE90) after 90 min of enzyme treatment. The same parameters have been measured for each of the four pollutants with the same fluidized bed bioreactor loaded with tyrosinase-PAN beads. The internal comparison, i.e., in each of the two catalytic systems, has shown that both enzymes exhibit a removal efficiency in the following order BPF>BPA>BPB>TCBPA. The external comparison, i.e., the comparison between the two catalytic system, has shown that the catalytic power of laccase were higher than that of tyrosinase. The operational stability of both catalytic systems resulted excellent, since they maintained more than 80% of the initial activity after 30 days of work.

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Acknowledgments

This work has been carried out under the cooperation agreement between the University “Prof. Dr. Assen Zlatarov” of Burgas (Bulgaria) and the Italian Interuniversity Consortium “National Institute for Biostructures and Biosystems” (INBB). This work was also supported by scientific research sector of University “Prof. Dr Assen Zlatarov” of Burgas, by the Italian Ministry of Health/ISPESL under the National Strategic Project “Salute della donna”, by the Italian Ministry of Health/ISZM (Portici-Italy) and by the MIUR through a PRIN project (Funds 2008—Diano).

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Authors and Affiliations

  1. Institute of Genetic and Biophysics “ABT” of CNR, Via P. Castellino, 111, 80131, Naples, Italy

    Carla Nicolucci, Sergio Rossi, Umberto Bencivenga, Damiano G. Mita & Nadia Diano

  2. National Institute of Biosystems and Biostructures (INBB), Viale Medaglie d’Oro 305, 00136, Rome, Italy

    Carla Nicolucci, Ciro Menale, Mariangela Bianco, Luigi Mita, Damiano G. Mita & Nadia Diano

  3. Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138, Naples, Italy

    Ciro Menale, Mariangela Bianco, Luigi Mita, Damiano G. Mita & Nadia Diano

  4. Department of Biotechnology, University “Prof. Dr. A. Zlatarov”, Prof. Y. Yakimov Str. 1, 8010, Bourgas, Bulgaria

    Tzonka Godjevargova & Yavor Ivanov

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  1. Carla Nicolucci
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  2. Sergio Rossi
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  3. Ciro Menale
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  9. Damiano G. Mita
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Correspondence to Nadia Diano.

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Nicolucci, C., Rossi, S., Menale, C. et al. Biodegradation of bisphenols with immobilized laccase or tyrosinase on polyacrylonitrile beads. Biodegradation 22, 673–683 (2011). https://doi.org/10.1007/s10532-010-9440-2

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  • DOI: https://doi.org/10.1007/s10532-010-9440-2

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