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Influence of sampling sufficiency on biodiversity analysis of microperiphyton communities for marine bioassessment

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Abstract

Introduction

With quick responses to environmental changes, easy sampling, relative immobility, increasing availability of easily used taxonomic references, and allowing standardization for temporal and spatial comparisons, the biodiversity measures of microperiphyton communities have widely been accepted as useful indicators to evaluate environmental stress and anthropogenic impact.

Materials and methods

The influence of sampling sufficiency for biodiversity analysis of microperiphyton communities was studied using a range of statistical methods in coastal waters of the Yellow Sea, northern China, from May to June 2010. Samples were collected from two depths using an artificial substrate.

Results

Sampling sizes represented a significant influence on biodiversity analysis of microperiphyton communities, e.g., 20 slide replicates (350 cm2) were sufficient for the microperiphyton communities at both depths, while 10 slide replicates (175 cm2) could meet the sampling strategy only for the samples with colonization times of 10 days or more at a depth of 1 m for recovering 90% species during the study period. Otherwise, more slide replicates were required with the increase of water depths and shortening colonization times for recovering microperiphyton species, e.g., for recovering 90% species of a “mature” microperiphyton community (>10 days), ∼10 slide replicates (∼175 cm2) were sufficient at a depth of 1 m, while for the “young” samples (>10 days) much more (15–30) slide replicates were required at both depths in this study. Furthermore, to achieve <10% standard errors, six (105 cm2) and nine (∼160 cm2) slide replicates were required for biodiversity analysis of the microperiphyton communities with various colonization times at depths of 1 and 3 m during the summer season, respectively.

Conclusion

These results suggest that sampling sizes represented a significant influence on biodiversity analysis of microperiphyton communities for monitoring programs and ecological conservation researches in marine ecosystems.

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Acknowledgments

This work was supported by “The Natural Science Foundation of China” (project no. 41076089), and funded by Research Group (project no. RGP-VPP-083), King Saud University Deanship of Scientific Research. Special thanks are due to Prof. Weibo Song, Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, for helpful discussions in the preparation of the manuscript.

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Authors and Affiliations

  1. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Henglong Xu, Wei Zhang, Yong Jiang & Mingzhuang Zhu

  2. Zoology Department, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia

    Khaled A. S. Al-Rasheid

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  1. Henglong Xu
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  2. Wei Zhang
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  3. Yong Jiang
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  4. Mingzhuang Zhu
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  5. Khaled A. S. Al-Rasheid
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Correspondence to Henglong Xu.

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Responsible editor: Philippe Garrigues

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Xu, H., Zhang, W., Jiang, Y. et al. Influence of sampling sufficiency on biodiversity analysis of microperiphyton communities for marine bioassessment. Environ Sci Pollut Res 19, 540–549 (2012). https://doi.org/10.1007/s11356-011-0578-1

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  • DOI: https://doi.org/10.1007/s11356-011-0578-1

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