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Transplantation of Microbenthic Algal Assemblages to Assess Structural and Functional Recovery After Diuron Exposure

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Abstract

The potential of microbenthic algal assemblages to recover after diuron exposure was investigated. Microbenthic algal assemblages (periphyton) were grown on glass slides in correspondence to a diuron-polluted and a diuron-free sampling site of a river. After 5 weeks of colonization, the impacted periphyton was transferred by translocating the colonized glass slides to the unpolluted site. To monitor the changes in functional and structural parameters and to assess recovery, both the transferred and the local reference periphyton were sampled at the day of transfer (t0) and 1, 3, and 5 weeks after the transfer (t1, t3, t5). Structural transitions of eukaryotic communities were characterized by denaturing gradient gel electrophoresis (DGGE) and functional ones were characterized by short-term incubation toxicity tests with diuron. As shown by Bray–Curtis similarity values based on DGGE band patterns, almost total structural recovery of the transferred periphyton took place 5 weeks after transfer. For the transferred periphyton, previous diuron exposure at the contaminated site induced the development of diuron tolerance, which decreased after 1 week and became very similar to the tolerance of the nonimpacted community after 5 weeks. It is concluded that 5-week-old eukaryotic periphyton communities are capable of restoring their structural and functional attributes after 5 weeks within a noncontaminated environment.

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References

  • AFNOR (1982) Essais des eaux: Norme Française. NFT 90-023. AFNOR

  • Barranguet C, Plans M, van der Grinten E, Sinke JJ, Admiraal W (2002) Development of photosynthetic biofilms affected by dissolved and sorbed copper in a eutrophic river. Environ Toxicol Chem 21:1955–1965

    Article  CAS  Google Scholar 

  • Bérard A, Pelte T, Druart JC (1999) Seasonal variations in the sensitivity of Lake Geneva phytoplankton community structure to atrazine. Arch Hydrobiol 145:277–295

    Google Scholar 

  • Biggs BJF (2000) New Zealand periphyton guideline: detecting, monitoring and managing enrichment of streams. Ministry for the Environment, New Zealand

  • Biondini ME, Bonham CD, Redente EF (1985) Secondary successional patterns in a sagebrush (Artemisia tridentata) community as they relate to soil disturbance and soil biological activity. Vegetatio 60:25–36

    Article  Google Scholar 

  • Blanck H (2002) A critical review of procedures and approaches used for assessing pollution-induced community tolerance (PICT) in biotic communities. Hum Ecol Risk Assess 8:1003–1034

    Article  Google Scholar 

  • Blanck H, Dahl B (1998) Recovery of marine periphyton communities around a Swedish marina after the ban of TBT use in antifouling paint. Marine Pollut Bull 36:437–442

    Article  CAS  Google Scholar 

  • Blanck H, Wänkberg SÅ, Molander S (1988) Pollution-induced community tolerance: a new ecotoxicological tool. In: Cairs J Jr, Pratt JR (eds) Functional testing of aquatic biota for estimating hazards of chemicals. STP 988. ASTM, Philadelphia, pp 219–230

    Chapter  Google Scholar 

  • Boivin ME, Massieux B, Breure AM, Greve GD, Rutgers M, Admiraal W (2006) Functional recovery of biofilm bacterial communities after copper exposure. Environ Pollut 140:239–246

    Article  CAS  Google Scholar 

  • Casamayor EO, Schäfer H, Bañeras L, Pedrós-Alió C, Muyzer G (2000) Identification of spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis. Appl Environ Microbiol 66:499–508

    Article  CAS  Google Scholar 

  • Cattaneo A, Kerimian T, Roberge M, Marty J (1997) Periphyton distribution and abundance on substrata of different size along a gradient of stream trophy. Hydrobiologia 354:101–110

    Article  CAS  Google Scholar 

  • Dorigo U, Leboulanger C (2001) A pulse-amplitude modulated fluorescence-based method for assessing the effects of photosystem II herbicides on freshwater periphyton. J Appl Phycol 13:509–515

    Article  CAS  Google Scholar 

  • Dorigo U, Fontvieille D, Humbert JF (2006) Spatial variability in the dynamic and composition of the free-living bacterioplankton community in the pelagic zone of Lake Bourget (France). FEMS Microbiol Ecol 58:109–119

    Article  CAS  Google Scholar 

  • Dorigo U, Leboulanger C, Bérard A, Bouchez A, Humbert JF, Montuelle B (2007) Lotic biofilm community structure and pesticide tolerance along a contamination gradient in a vineyard area. Aquat Microb Ecol 50:91–102

    Article  Google Scholar 

  • Dorigo U, Lefranc M, Leboulanger C, Montuelle B, Humbert JF (2009) Spatial heterogeneity of periphytic microbial communities in a small pesticide-polluted river. FEMS Microbiol Ecol 67:491–501

    Article  CAS  Google Scholar 

  • Eulin A, Le Cohu R (1998) Epilithic diatom communities during the colonization of artificial substrates in the River Garonne (France). Comparison with natural communities. Arch Hybrobiol 143:76–106

    Google Scholar 

  • Francoeur SN, Biggs BJF (2006) Short-term effects of elevated velocity and sediment abrasion on benthic algal communities. Hydrobiologia 561:59–69

    Article  Google Scholar 

  • Gouy V, Nivon C (2006) Protection des eaux en Beaujolais viticole: caractérisation et suivi de la qualité des eaux sur le bassin versant de la Morcille sur la période 2001–2005. Cemagref, Rapport final d’étude. Cemagref, Lyon

  • Guasch H, Ivorra N, Paulsson M, Montserrat R, Sabater S (1998) Community composition and sensitivity of periphyton to atrazine in flowing waters: the role of environmental factors. J Appl Phycol 10:203–213

    Article  Google Scholar 

  • Headley JV, Gandrass J, Kuballa J, Peru KM, Gong Y (1998) Rates of sorption and partitioning of contaminants in river biofilm. Environ Sci Technol 32:3968–3973

    Article  CAS  Google Scholar 

  • Hoagland KD, Roemer SC, Rosowski JR (1982) Colonization and community structure of two periphyton assemblages, with emphasis on the diatoms (Bacillariophyceae). Am J Bot 69:188–213

    Article  Google Scholar 

  • Iserentant R, Blancke D (1984) A transplantation experiment in running water to measure the response rate of diatoms to changes in water quality. In: Ricard M (ed) Proceedings of the 18th international diatom symposium, Paris. Koeltz Scientific Books, Koenigstein

    Google Scholar 

  • Ivorra N, Hettelaar J, Tubbing GM, Kraak MH, Sabater S, Admiraal W (1999) Translocation of microbenthic algal assemblages used for in situ analysis of metal pollution in rivers. Arch Environ Contam Toxicol 37:19–28

    Article  CAS  Google Scholar 

  • Jurgensen TA, Hoagland KD (1990) Effects of short-term pulses of atrazine on attached algal communities in a small stream. Arch Environ Contam Toxicol 19:620–623

    Article  Google Scholar 

  • Knauer K, Leimgruber A, Hommen U, Knauert S (2010) Co-tolerance of phytoplankton communities to photosynthesis II inhibitors. Aquat Toxicol 96(4):256–263

    Article  CAS  Google Scholar 

  • McCune B, Mefford J (2006) PC-ORD. Multivariate analysis of ecological data, version 5.18. MjM Software, Gleneden Beach, OR

  • Molander S (1991) Detection, validity and specificity of pollution-induced community tolerance. PhD thesis, Department of Plant Physiology, Göteburg University, Sweden

  • Montuelle B, Dorigo U, Bérard A, Volat B, Bouchez A, Tlili A, Gouy V, Pesce S (2010) The periphyton as a multimetric bioindicator for assessing the impact of land use on rivers: an overview on the Ardières–Morcille experimental watershed (France). Hydrobiologia. doi:10.1007/s10750-010-0105-2

  • Morin S, Duong TT, Herlory O, Feurtet-Mazel A, Coste M (2008) Cadmium toxicity and bioaccumulation in freshwater biofilms. Arch Environ Contam Toxicol 54:173–186

    Article  CAS  Google Scholar 

  • Niemi GJ, Devore P, Detenbeck N, Taylor D, Lima A, Pastor J, Yount JD, Naiman RJ (1990) Overview of case studies on recovery of aquatic systems from disturbance. Environ Manag 14:571–587

    Article  Google Scholar 

  • Patil JS, Anil AC (2005) Quantification of diatoms in biofilms: standardisation of methods. Biofouling 21:181–188

    Article  CAS  Google Scholar 

  • Paule A, Lyautey E, Garabetian F, Rols JL (2009) Autogenic versus environmental control during development of river biofilm. Ann Limnol 45:1–10

    Article  Google Scholar 

  • Pesce S, Margoum C, Montuelle B (2010). In situ relationship between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river. Water Res. doi:10.1016/j.waters.2009.11.053

  • Porsbring T (2009) On toxicant-induced succession in periphyton communities: effects of single chemicals and chemical mixtures. Dissertation, University of Gothenburg

  • Rimet F (2009) Benthic diatom assemblages and their correspondence with ecoregional classifications: case study of rivers in north-eastern France. Hydrobiologia 636:137–151

    Article  Google Scholar 

  • Rimet F, Cauchie HM, Hoffmann L, Ector L (2005) Response of diatom indices to simulated water quality improvements in a river. J Appl Physicol 17:119–128

    Article  Google Scholar 

  • Sabater S, Gregory S, Sedell J (1998) Community dynamics and metabolism of benthic algae colonizing wood and rock substrata in a forest stream. J Phycol 34:561–567

    Article  Google Scholar 

  • Sabater S, Guasch H, Ricart M, Romani A, Vidal G, Klünder C, Schmitt-Jansen M (2007) Monitoring the effect of chemicals on biological communities. The biofilm as an interface. Anal Bioanal Chem 387:1425–1434

    Article  CAS  Google Scholar 

  • Schmitt-Jansen M, Altenburger R (2005a) Predicting and observing responses of algal communities to photosystem II-herbicide exposure using pollution-induced community tolerance and species-sensitivity distributions. Environ Toxicol Chem 24:304–312

    Article  CAS  Google Scholar 

  • Schmitt-Jansen M, Altenburger R (2005b) Toxic effects of isoproturon on periphyton communities: a microcosm study. Estuar Coast Shelf 62:539–545

    Article  CAS  Google Scholar 

  • Seguin F, Leboulanger C, Rimet F, Druart JC, Bérard A (2001) Effects of atrazine and nicosulfuron on phytoplankton in systems of increasing complexity. Arch Environ Contam Toxicol 40:198–208

    Article  CAS  Google Scholar 

  • Soininen J (2004) Assessing the current related heterogeneity and diversity patterns of benthic diatom communities in a turbid and clean water river. Aquat Ecol 38:495–501

    Article  Google Scholar 

  • Stevenson RJ (1990) Benthic algal dynamics in a stream during and after a spate. J N Am Benthol Soc 9:277–288

    Article  Google Scholar 

  • Stevenson RJ, Peterson CG, Kirschtel DB, King CC, Tuchman NC (1991) Density-dependent growth, ecological strategies, and effects of nutrients and shading on benthic diatom succession in streams. J Physicol 27:59–69

    Google Scholar 

  • Tlili A, Dorigo U, Montuelle B, Margoum C, Carluer N, Gouy V, Bouchez A, Bérard A (2008) Responses of chronically contaminated biofilms to short pulses of diuron. An experimental study simulating flooding events in a small river running through a vineyard watershed. Aquat Toxicol 87:252–263

    Article  CAS  Google Scholar 

  • Tlili A, Bérard A, Roullier JL, Volat B, Montuelle B (2010) PO43− dependence of the tolerance of autotrophic and heterotrophic biofilm communities to copper and diuron. Aquat Toxicol. doi:10.1016/j.aquatox.2010.02.008

  • Tolcach ER, Gomez N (2002) The effect of translocation of microbenthic communities in a polluted lowland stream. Verh Int Ver Theor Limnol 28:254–258

    Google Scholar 

  • Villeneuve A, Montuelle B, Bouchez A (2010) Influence of slight differences in environmental conditions (light, hydrodynamics) on the structure and function of the periphyton. Aquat Sci 72:33–44

    Article  Google Scholar 

  • Wellnitz T, Rader RB (2003) Mechanisms influencing community composition and succession in mountain stream periphyton: interactions between scouring history, grazing and irradiance. J N Am Benthol Soc 22:528–541

    Article  Google Scholar 

  • Xu FL, Jorgensen SE, Tao S (1999) Ecological indicators for assessing freshwater ecosystem health. Ecol Model 116:77–106

    Article  CAS  Google Scholar 

  • Zimba PV, Tucker CS, Mischke CC, Grimm CC (2002) Short-term effects of diuron on catfish pond ecology. N Am J Aquacult 64:16–23

    Article  Google Scholar 

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Acknowledgments

The authors acknowledge B. Motte, and B. Volat for their support for field sampling and for their help in the laboratory. We also thank the Water Chemistry Laboratory of the CEMAGREF station in Lyon, which carried out nutrient and pesticide analyses in water. The project received funding from the national program ECOGER-PAPIER (AIP INRA) and was also supported by the LTER Zone Atelier du Bassin du Rhône (ZABR).

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Authors and Affiliations

  1. Cemagref, UR MALY, 3 Quai Chauveau, CP220, 69336, Lyon Cedex, France

    Ursula Dorigo, Annette Bérard & Bernard Montuelle

  2. INRA, UMR CARRTEL, 75 av. de Corzent, BP 511, 74203, Thonon Cedex, France

    Ursula Dorigo, Agnès Bouchez, Frédéric Rimet & Bernard Montuelle

  3. INRA, UMR INRA/UAPV Climat Sol Environnement, Site Agroparc, 84914, Avignon Cedex, France

    Annette Bérard

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  1. Ursula Dorigo
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Correspondence to Agnès Bouchez.

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Dorigo, U., Bérard, A., Bouchez, A. et al. Transplantation of Microbenthic Algal Assemblages to Assess Structural and Functional Recovery After Diuron Exposure. Arch Environ Contam Toxicol 59, 555–563 (2010). https://doi.org/10.1007/s00244-010-9511-8

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  • DOI: https://doi.org/10.1007/s00244-010-9511-8

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